Summary of Study ST000287
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000229. The data can be accessed directly via it's Project DOI: 10.21228/M8259N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000287 |
| Study Title | Energetics in dystrophic muscle |
| Study Type | Cross-sectional |
| Study Summary | This project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion. |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | Muscle Physiology Laboratory |
| Last Name | Forbes |
| First Name | Sean |
| Address | University of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC |
| scforbes@ufl.edu | |
| Phone | 352-273-6111 |
| Submit Date | 2015-09-30 |
| Num Groups | 4 |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000229 |
| Project DOI: | doi: 10.21228/M8259N |
| Project Title: | Energetics in dystrophic muscle |
| Project Type: | Cross-sectional |
| Project Summary: | This project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion. |
| Institute: | University of Florida |
| Department: | Physical Therapy |
| Laboratory: | Muscle Physiology Laboratory |
| Last Name: | Forbes |
| First Name: | Sean |
| Address: | University of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC |
| Email: | scforbes@ufl.edu |
| Phone: | 352-273-6111 |
| Funding Source: | Start-up funds |
Subject:
| Subject ID: | SU000307 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Lab |
| Animal Housing: | McKnight Brain Institute, UF |
| Animal Light Cycle: | 12 hr light/12 hr dark |
| Animal Feed: | ad libtum |
| Animal Water: | ad libtum |
| Animal Inclusion Criteria: | males only |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Strain |
|---|---|---|
| SA013114 | scf_04-M1 | C57Bl/10 |
| SA013115 | scf_04-M2 | C57Bl/10 |
| SA013116 | scf_04-M3 | C57Bl/10 |
| SA013117 | scf_04-M4 | C57Bl/10 |
| SA013118 | scf_04-M5 | C57Bl/10 |
| SA013119 | scf_03-M3 | mdx |
| SA013120 | scf_03-M4 | mdx |
| SA013121 | scf_03-M5 | mdx |
| SA013122 | scf_03-M2 | mdx |
| SA013123 | scf_03-M1 | mdx |
| SA013124 | scf_02-M1 | mdx-nNOS-/- |
| SA013125 | scf_02-M3 | mdx-nNOS-/- |
| SA013126 | scf_02-M2 | mdx-nNOS-/- |
| SA013127 | scf_02-M4 | mdx-nNOS-/- |
| SA013128 | scf_02-M5 | mdx-nNOS-/- |
| SA013129 | scf_01-M2 | nNOS-/- |
| SA013130 | scf_01-M3 | nNOS-/- |
| SA013131 | scf_01-M5 | nNOS-/- |
| SA013132 | scf_01-M1 | nNOS-/- |
| SA013133 | scf_01-M4 | nNOS-/- |
| Showing results 1 to 20 of 20 |
Collection:
| Collection ID: | CO000301 |
| Collection Summary: | Muscles extracted under isoflurane, snap frozen with liquid nitrogen, stored at -80C |
| Collection Protocol Comments: | L3-190 |
| Sample Type: | Muscle |
| Collection Method: | muscle extraction, frozen |
| Collection Location: | UF, musle physiology laboratory |
| Collection Frequency: | once/mouse |
| Collection Duration: | Total ~5 min |
| Collection Time: | no dose |
| Volumeoramount Collected: | entire muscles extracted |
| Storage Conditions: | -80C freezer |
| Collection Vials: | 2 ml vials |
| Storage Vials: | 2 ml vials |
| Collection Tube Temp: | -80C |
| Additives: | none |
| Blood Serum Or Plasma: | none |
| Tissue Cell Identification: | none |
| Tissue Cell Quantity Taken: | none |
Treatment:
| Treatment ID: | TR000321 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP000315 |
| Sampleprep Summary: | NAD/ATP or NADH |
| Sampleprep Protocol Filename: | SB_NAD-ATP_Assay.pdf, SB_NADH_Assay_Agilent.pdf |
Combined analysis:
| Analysis ID | AN000456 | AN000457 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Thermo Hypercarb (50 x 2.1mm,3um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ |
| Ion Mode | POSITIVE | POSITIVE |
| Units | pmol/mg dry powder | pmol/mg dry powder |
Chromatography:
| Chromatography ID: | CH000325 |
| Chromatography Summary: | NAD-ATP method |
| Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000326 |
| Chromatography Summary: | NAD-ATP method |
| Methods Filename: | SB_NADH_Assay_Agilent.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Rate: | 0.54 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 6 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000396 |
| Analysis ID: | AN000456 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | NAD/ATP |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | SB_NAD-ATP_Assay_Agilent.pdf |
| MS ID: | MS000397 |
| Analysis ID: | AN000457 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | NADH |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | SB_NADH_Assay_Agilent.pdf |
