Summary of Study ST000287

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000229. The data can be accessed directly via it's Project DOI: 10.21228/M8259N This work is supported by NIH grant, U2C- DK119886.

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Study IDST000287
Study TitleEnergetics in dystrophic muscle
Study TypeCross-sectional
Study SummaryThis project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion.
Institute
University of Florida
DepartmentSECIM
LaboratoryMuscle Physiology Laboratory
Last NameForbes
First NameSean
AddressUniversity of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC
Emailscforbes@ufl.edu
Phone352-273-6111
Submit Date2015-09-30
Num Groups4
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Sean Forbes Sean Forbes
https://dx.doi.org/10.21228/M8259N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000229
Project DOI:doi: 10.21228/M8259N
Project Title:Energetics in dystrophic muscle
Project Type:Cross-sectional
Project Summary:This project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion.
Institute:University of Florida
Department:Physical Therapy
Laboratory:Muscle Physiology Laboratory
Last Name:Forbes
First Name:Sean
Address:University of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC
Email:scforbes@ufl.edu
Phone:352-273-6111
Funding Source:Start-up funds

Subject:

Subject ID:SU000307
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male
Animal Animal Supplier:Jackson Lab
Animal Housing:McKnight Brain Institute, UF
Animal Light Cycle:12 hr light/12 hr dark
Animal Feed:ad libtum
Animal Water:ad libtum
Animal Inclusion Criteria:males only
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Strain
SA013114scf_04-M1C57Bl/10
SA013115scf_04-M2C57Bl/10
SA013116scf_04-M3C57Bl/10
SA013117scf_04-M4C57Bl/10
SA013118scf_04-M5C57Bl/10
SA013119scf_03-M3mdx
SA013120scf_03-M4mdx
SA013121scf_03-M5mdx
SA013122scf_03-M2mdx
SA013123scf_03-M1mdx
SA013124scf_02-M1mdx-nNOS-/-
SA013125scf_02-M3mdx-nNOS-/-
SA013126scf_02-M2mdx-nNOS-/-
SA013127scf_02-M4mdx-nNOS-/-
SA013128scf_02-M5mdx-nNOS-/-
SA013129scf_01-M2nNOS-/-
SA013130scf_01-M3nNOS-/-
SA013131scf_01-M5nNOS-/-
SA013132scf_01-M1nNOS-/-
SA013133scf_01-M4nNOS-/-
Showing results 1 to 20 of 20

Collection:

Collection ID:CO000301
Collection Summary:Muscles extracted under isoflurane, snap frozen with liquid nitrogen, stored at -80C
Collection Protocol Comments:L3-190
Sample Type:Muscle
Collection Method:muscle extraction, frozen
Collection Location:UF, musle physiology laboratory
Collection Frequency:once/mouse
Collection Duration:Total ~5 min
Collection Time:no dose
Volumeoramount Collected:entire muscles extracted
Storage Conditions:-80C freezer
Collection Vials:2 ml vials
Storage Vials:2 ml vials
Collection Tube Temp:-80C
Additives:none
Blood Serum Or Plasma:none
Tissue Cell Identification:none
Tissue Cell Quantity Taken:none

Treatment:

Treatment ID:TR000321
Treatment Summary:No treatment

Sample Preparation:

Sampleprep ID:SP000315
Sampleprep Summary:NAD/ATP or NADH
Sampleprep Protocol Filename:SB_NAD-ATP_Assay.pdf, SB_NADH_Assay_Agilent.pdf

Combined analysis:

Analysis ID AN000456 AN000457
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Thermo Hypercarb (50 x 2.1mm, 3um) Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name Agilent 6490 QQQ Agilent 6490 QQQ
Ion Mode POSITIVE POSITIVE
Units pmol/mg dry powder pmol/mg dry powder

Chromatography:

Chromatography ID:CH000325
Chromatography Summary:NAD-ATP method
Methods Filename:SB_NAD-ATP_Assay_Agilent.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Thermo Hypercarb (50 x 2.1mm, 3um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000326
Chromatography Summary:NAD-ATP method
Methods Filename:SB_NADH_Assay_Agilent.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000396
Analysis ID:AN000456
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:NAD/ATP
Ion Mode:POSITIVE
Analysis Protocol File:SB_NAD-ATP_Assay_Agilent.pdf
  
MS ID:MS000397
Analysis ID:AN000457
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:NADH
Ion Mode:POSITIVE
Analysis Protocol File:SB_NADH_Assay_Agilent.pdf
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