Summary of Study ST000287

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000229. The data can be accessed directly via it's Project DOI: 10.21228/M8259N This work is supported by NIH grant, U2C- DK119886.

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Study IDST000287
Study TitleEnergetics in dystrophic muscle
Study TypeCross-sectional
Study SummaryThis project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion.
Institute
University of Florida
DepartmentSECIM
LaboratoryMuscle Physiology Laboratory
Last NameForbes
First NameSean
AddressUniversity of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC
Emailscforbes@ufl.edu
Phone352-273-6111
Submit Date2015-09-30
Num Groups4
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Sean Forbes Sean Forbes
https://dx.doi.org/10.21228/M8259N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000229
Project DOI:doi: 10.21228/M8259N
Project Title:Energetics in dystrophic muscle
Project Type:Cross-sectional
Project Summary:This project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion.
Institute:University of Florida
Department:Physical Therapy
Laboratory:Muscle Physiology Laboratory
Last Name:Forbes
First Name:Sean
Address:University of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC
Email:scforbes@ufl.edu
Phone:352-273-6111
Funding Source:Start-up funds

Subject:

Subject ID:SU000307
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male
Animal Animal Supplier:Jackson Lab
Animal Housing:McKnight Brain Institute, UF
Animal Light Cycle:12 hr light/12 hr dark
Animal Feed:ad libtum
Animal Water:ad libtum
Animal Inclusion Criteria:males only
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Strain
SA013114scf_04-M1C57Bl/10
SA013115scf_04-M2C57Bl/10
SA013116scf_04-M3C57Bl/10
SA013117scf_04-M4C57Bl/10
SA013118scf_04-M5C57Bl/10
SA013119scf_03-M3mdx
SA013120scf_03-M4mdx
SA013121scf_03-M5mdx
SA013122scf_03-M2mdx
SA013123scf_03-M1mdx
SA013124scf_02-M1mdx-nNOS-/-
SA013125scf_02-M3mdx-nNOS-/-
SA013126scf_02-M2mdx-nNOS-/-
SA013127scf_02-M4mdx-nNOS-/-
SA013128scf_02-M5mdx-nNOS-/-
SA013129scf_01-M2nNOS-/-
SA013130scf_01-M3nNOS-/-
SA013131scf_01-M5nNOS-/-
SA013132scf_01-M1nNOS-/-
SA013133scf_01-M4nNOS-/-
Showing results 1 to 20 of 20

Collection:

Collection ID:CO000301
Collection Summary:Muscles extracted under isoflurane, snap frozen with liquid nitrogen, stored at -80C
Collection Protocol Comments:L3-190
Sample Type:Muscle
Collection Method:muscle extraction, frozen
Collection Location:UF, musle physiology laboratory
Collection Frequency:once/mouse
Collection Duration:Total ~5 min
Collection Time:no dose
Volumeoramount Collected:entire muscles extracted
Storage Conditions:-80C freezer
Collection Vials:2 ml vials
Storage Vials:2 ml vials
Collection Tube Temp:-80C
Additives:none
Blood Serum Or Plasma:none
Tissue Cell Identification:none
Tissue Cell Quantity Taken:none

Treatment:

Treatment ID:TR000321
Treatment Summary:No treatment

Sample Preparation:

Sampleprep ID:SP000315
Sampleprep Summary:NAD/ATP or NADH
Sampleprep Protocol Filename:SB_NAD-ATP_Assay.pdf, SB_NADH_Assay_Agilent.pdf

Combined analysis:

Analysis ID AN000456 AN000457
Chromatography ID CH000325 CH000326
MS ID MS000396 MS000397
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Thermo Hypercarb (50 x 2.1mm,3um) Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name Agilent 6490 QQQ Agilent 6490 QQQ
Ion Mode POSITIVE POSITIVE
Units pmol/mg dry powder pmol/mg dry powder

Chromatography:

Chromatography ID:CH000325
Chromatography Summary:NAD-ATP method
Methods Filename:SB_NAD-ATP_Assay_Agilent.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Thermo Hypercarb (50 x 2.1mm,3um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000326
Chromatography Summary:NAD-ATP method
Methods Filename:SB_NADH_Assay_Agilent.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:30
Flow Rate:0.54 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 6
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000396
Analysis ID:AN000456
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:NAD/ATP
Ion Mode:POSITIVE
Analysis Protocol File:SB_NAD-ATP_Assay_Agilent.pdf
  
MS ID:MS000397
Analysis ID:AN000457
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:NADH
Ion Mode:POSITIVE
Analysis Protocol File:SB_NADH_Assay_Agilent.pdf
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