Summary of Study ST000364

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000289. The data can be accessed directly via it's Project DOI: 10.21228/M8PK67 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis | Show all samples | Download binned data
Download mwTab file (text) | Download mwTab file(JSON) | Download data files (Contains raw data)

Study ID	ST000364
Study Title	Metabolomics Profiling of NEST Cord Blood Plasma
Study Type	Metabolomics
Study Summary	This metabolomics pilot study evaluated cord blood plasma from these children to understand how these factors influence the metabotype.
Institute	University of North Carolina
Laboratory	Sumner Lab
Last Name	Sumner
First Name	Susan
Address	Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Email	susan_sumner @unc.edu
Phone	704-250-5066
Submit Date	2016-03-09
Raw Data Available	Yes
Raw Data File Type(s)	1r
Analysis Type Detail	NMR
Release Date	2017-10-03
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000289
Project DOI:	doi: 10.21228/M8PK67
Project Title:	The Newborn Epigenetic STudy (NEST)
Project Type:	Epigenetic
Project Summary:	The overall goals of the Newborn Epigenetic STudy (NEST) research study are to identify epigenetic features in the genome that affect gene regulation and that can be altered by endogenous and exogenous influences. We hypothesize that such differences in the global epigenetic profile may be due to differences in race, maternal behaviors (e.g., nutritional status, cigarette smoking, etc.) before and throughout pregnancy, or result from conception via assisted reproductive technologies.
Institute:	North Carolina State University
Last Name:	Hoyo
First Name:	Cathrine
Address:	"Box 7614 NCSU Campus Raleigh, NC 27695"
Email:	choyo@ncsu.edu
Phone:	919-515-0540

Subject:

Subject ID:	SU000385
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Pool	Metal Exposure	Gender	Prenatal Smoking	Race	Asthma
SA016336	S_79	-	Control	Female	-	Black	-
SA016337	S_49	-	Control	Female	-	Hispanic	-
SA016338	S_51	-	Control	Female	-	Other	-
SA016339	S_32	-	Control	Female	-	White	-
SA016340	S_69	-	Control	Female	-	White	-
SA016341	S_67	-	Control	Female	-	White	-
SA016342	S_42	-	Control	Female	-	White	-
SA016343	S_63	-	Control	Male	-	Black	-
SA016344	S_3	-	Control	Male	-	Black	-
SA016345	S_83	-	Control	Male	-	Black	-
SA016346	S_75	-	Control	Male	-	Hispanic	-
SA016347	S_60	-	Control	Male	-	Hispanic	-
SA016348	S_57	-	Control	Male	-	Hispanic	-
SA016349	S_38	-	Control	Male	-	Hispanic	-
SA016325	S_11	-	-	Female	1	Black	-
SA016320	S_68	-	-	Female	-	Black	-
SA016321	S_23	-	-	Female	-	Black	1
SA016322	S_98	-	-	Female	-	Black	1
SA016323	S_61	-	-	Female	-	Black	1
SA016324	S_86	-	-	Female	-	Hispanic	1
SA016334	S_93	-	-	Male	1	Black	-
SA016335	S_80	-	-	Male	1	White	-
SA016326	S_41	-	-	Male	-	Black	-
SA016327	S_40	-	-	Male	-	Black	-
SA016328	S_24	-	-	Male	-	Black	1
SA016329	S_6	-	-	Male	-	Black	1
SA016330	S_81	-	-	Male	-	Black	1
SA016331	S_77	-	-	Male	-	Black	1
SA016332	S_44	-	-	Male	-	Black	1
SA016333	S_94	-	-	Male	-	White	-
SA016358	S_91	-	high Cd, low Pb = Cd 75th pct+, Pb 5-25th pct	Female	1	Black	-
SA016359	S_101	-	high Cd, low Pb = Cd 75th pct+, Pb 5-25th pct	Female	1	White	-
SA016360	S_34	-	high Cd, low Pb = Cd 75th pct+, Pb 5-25th pct	Male	1	White	-
SA016353	S_76	-	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Female	1	Hispanic	-
SA016354	S_20	-	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Female	1	White	-
SA016350	S_58	-	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Female	-	Black	-
SA016351	S_1	-	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Female	-	Hispanic	-
SA016352	S_39	-	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Female	-	Hispanic	1
SA016355	S_52	-	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Male	-	Hispanic	-
SA016356	S_33	-	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Male	-	Hispanic	-
SA016357	S_108	-	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Male	-	Hispanic	1
SA016361	S_64	-	high Pb, low Cd = Pb 75th pct+, Cd 5-25th	Female	-	Hispanic	-
SA016362	S_82	-	high Pb, low Cd = Pb 75th pct+, Cd 5-25th	Female	-	Other	1
SA016363	S_78	-	high Pb, low Cd = Pb 75th pct+, Cd 5-25th	Female	-	White	1
SA016364	S_97	-	high Pb, low Cd = Pb 75th pct+, Cd 5-25th	Female	-	White	1
SA016365	S_90	-	high Pb, low Cd = Pb 75th pct+, Cd 5-25th	Male	-	Hispanic	-
SA016366	S_48	-	high Pb, low Cd = Pb 75th pct+, Cd 5-25th	Male	-	White	-
SA016367	S_104	-	high Pb, low Cd = Pb 75th pct+, Cd 5-25th	Male	-	White	-
SA016368	S_10	-	high Pb, low Cd = Pb 75th pct+, Cd 5-25th	Male	-	White	-
SA016369	S_85	-	high Pb, no Cd = Pb 75th pct+, Cd 5th pct or less	Male	-	Hispanic	-
SA016400	P_9	Pool 1	-	-	-	-	-
SA016401	P_6	Pool 1	-	-	-	-	-
SA016402	P_3	Pool 1	-	-	-	-	-
SA016403	P_1	Pool 1	-	-	-	-	-
SA016404	P_8	Pool 1	-	-	-	-	-
SA016389	S_30	Pool 1 Sample	Control	Female	-	Hispanic	-
SA016390	S_25	Pool 1 Sample	Control	Male	-	Black	-
SA016375	S_47	Pool 1 Sample	-	Female	1	Black	1
SA016376	S_31	Pool 1 Sample	-	Female	1	White	-
SA016370	S_12	Pool 1 Sample	-	Female	-	Black	1
SA016371	S_29	Pool 1 Sample	-	Female	-	Black	1
SA016372	S_4	Pool 1 Sample	-	Female	-	Black	1
SA016373	S_9	Pool 1 Sample	-	Female	-	Hispanic	-
SA016374	S_26	Pool 1 Sample	-	Female	-	White	-
SA016388	S_37	Pool 1 Sample	-	Male	1	White	-
SA016377	S_14	Pool 1 Sample	-	Male	-	Black	-
SA016378	S_2	Pool 1 Sample	-	Male	-	Black	-
SA016379	S_43	Pool 1 Sample	-	Male	-	Black	1
SA016380	S_5	Pool 1 Sample	-	Male	-	Hispanic	-
SA016381	S_28	Pool 1 Sample	-	Male	-	Hispanic	1
SA016382	S_46	Pool 1 Sample	-	Male	-	Hispanic	1
SA016383	S_19	Pool 1 Sample	-	Male	-	Hispanic	1
SA016384	S_8	Pool 1 Sample	-	Male	-	White	-
SA016385	S_21	Pool 1 Sample	-	Male	-	White	-
SA016386	S_22	Pool 1 Sample	-	Male	-	White	-
SA016387	S_18	Pool 1 Sample	-	Male	-	White	-
SA016396	S_45	Pool 1 Sample	high Cd, low Pb = Cd 75th pct+, Pb 5-25th pct	Female	-	Hispanic	-
SA016397	S_7	Pool 1 Sample	high Cd, low Pb = Cd 75th pct+, Pb 5-25th pct	Female	-	White	-
SA016393	S_35	Pool 1 Sample	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Female	1	Black	-
SA016391	S_50	Pool 1 Sample	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Female	-	Black	-
SA016392	S_16	Pool 1 Sample	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Female	-	Hispanic	-
SA016394	S_17	Pool 1 Sample	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Male	-	Black	1
SA016395	S_36	Pool 1 Sample	high Cd & Pb = Cd 80th pct+, Pb 80th pct+	Male	-	Hispanic	-
SA016398	S_27	Pool 1 Sample	high Pb, low Cd = Pb 75th pct+, Cd 5-25th	Female	-	Hispanic	-
SA016399	S_15	Pool 1 Sample	high Pb, low Cd = Pb 75th pct+, Cd 5-25th	Male	-	Hispanic	1
SA016437	P_2	Pool 2	-	-	-	-	-
SA016438	P_10	Pool 2	-	-	-	-	-
SA016439	P_5	Pool 2	-	-	-	-	-
SA016440	P_7	Pool 2	-	-	-	-	-
SA016441	P_4	Pool 2	-	-	-	-	-
SA016419	S_105	Pool 2 Sample	Control	Female	-	Hispanic	-
SA016420	S_88	Pool 2 Sample	Control	Female	-	White	-
SA016421	S_113	Pool 2 Sample	Control	Male	-	Black	-
SA016422	S_62	Pool 2 Sample	Control	Male	-	White	-
SA016409	S_103	Pool 2 Sample	-	Female	1	Black	-
SA016405	S_107	Pool 2 Sample	-	Female	-	Black	1
SA016406	S_106	Pool 2 Sample	-	Female	-	White	-
SA016407	S_66	Pool 2 Sample	-	Female	-	White	-
SA016408	S_84	Pool 2 Sample	-	Female	-	White	-
SA016410	S_74	Pool 2 Sample	-	Male	-	Black	-

Showing page 1 of 2 Results: 1 2 Next Showing results 1 to 100 of 122

Collection:

Collection ID:	CO000379
Collection Summary:	Cord Blood Plasma
Sample Type:	Blood

Treatment:

Treatment ID:	TR000399
Treatment Summary:	NA

Sample Preparation:

Sampleprep ID:	SP000392
Sampleprep Summary:	An aliquot (125 μl) of thawed plasma from each study sample was mixed with 375 μl of methanol, vortexed, and centrifuged. 400 uL of the supernatant was dried in vacuum overnight. Each sample was reconstituted with 250 μL of 0.2 mM phosphate buffer (pH 7.5) containing 10% Chenomx Internal Standard - 0.5 mM DSS-d6. Two random study pools were created by mixing 30 μl of plasma from a subset of study samples. Five replicates were created of each pool for a total of ten pooled samples, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 10 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000392

Sampleprep Summary:

An aliquot (125 μl) of thawed plasma from each study sample was mixed with 375 μl of methanol, vortexed, and centrifuged. 400 uL of the supernatant was dried in vacuum overnight. Each sample was reconstituted with 250 μL of 0.2 mM phosphate buffer (pH 7.5) containing 10% Chenomx Internal Standard - 0.5 mM DSS-d6. Two random study pools were created by mixing 30 μl of plasma from a subset of study samples. Five replicates were created of each pool for a total of ten pooled samples, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 10 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Analysis:

Analysis ID:	AN000597
Analysis Type:	NMR
Num Factors:	78

NMR:

NMR ID:	NM000064
Analysis ID:	AN000597
Instrument Name:	Bruker Avance III
Instrument Type:	FT-NMR
NMR Experiment Type:	1D 1H
Spectrometer Frequency:	700 MHz