Summary of Study ST000372
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000134. The data can be accessed directly via it's Project DOI: 10.21228/M8HP4Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000372 |
Study Title | Measure change in metabolites based on diet and feeding status (part I) |
Study Type | Regular |
Study Summary | Flies were fed a normal diet or high-sugar (30% sucrose) diet for 10 days. Flies of each diet were flash frozen in liquid nitrogen as sated (refed for 3hrs on 400mM D-glucose) or after fasting for 24hrs. Heads and bodies were separated using a sieve-system and placed immediately on dry ice (total time app. 1min). We collected 50 heads per condition (4 conditions total) and 5 biological replicates |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2016-04-18 |
Num Groups | 4 |
Total Subjects | 18 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000134 |
Project DOI: | doi: 10.21228/M8HP4Z |
Project Title: | Fly Brain Studies |
Project Summary: | Measure change in metabolites according to diet and feeding state |
Institute: | University of Michigan |
Department: | MCDB LS&A |
Laboratory: | Dus Lab |
Last Name: | Dus |
First Name: | Monica |
Address: | Ann Arbor, MI |
Email: | mdus@umich.edu |
Phone: | 347-891-2287 |
Subject:
Subject ID: | SU000393 |
Subject Type: | Animal |
Subject Species: | Drosophila melanogaster |
Taxonomy ID: | 7227 |
Species Group: | Insects |
Factors:
Subject type: Animal; Subject species: Drosophila melanogaster (Factor headings shown in green)
mb_sample_id | local_sample_id | State | Diet |
---|---|---|---|
SA016950 | S00018443 | sated | high sugar |
SA016951 | S00018447 | sated | high sugar |
SA016952 | S00018446 | sated | high sugar |
SA016953 | S00018444 | sated | high sugar |
SA016954 | S00018445 | sated | high sugar |
SA016955 | S00018451 | sated | normal |
SA016956 | S00018449 | sated | normal |
SA016957 | S00018450 | sated | normal |
SA016958 | S00018448 | sated | normal |
SA016959 | S00018458 | starved | high sugar |
SA016960 | S00018459 | starved | high sugar |
SA016961 | S00018460 | starved | high sugar |
SA016962 | S00018457 | starved | high sugar |
SA016963 | S00018452 | starved | normal |
SA016964 | S00018453 | starved | normal |
SA016965 | S00018454 | starved | normal |
SA016966 | S00018455 | starved | normal |
SA016967 | S00018456 | starved | normal |
Showing results 1 to 18 of 18 |
Collection:
Collection ID: | CO000387 |
Collection Summary: | none |
Sample Type: | Tissues |
Treatment:
Treatment ID: | TR000407 |
Treatment Summary: | none |
Treatment Protocol Filename: | Acyl-CoA_-20150305.docx |
Sample Preparation:
Sampleprep ID: | SP000400 |
Sampleprep Summary: | none |
Sampleprep Protocol Filename: | Acyl-CoA_-20150305.docx |
Combined analysis:
Analysis ID | AN000606 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | None |
Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6410 QQQ |
Ion Mode | POSITIVE |
Units | pmol/µg tissue protein |
Chromatography:
Chromatography ID: | CH000432 |
Methods ID: | DQM0127 |
Methods Filename: | QM-005-Xbrdg2mm-CoA-6490.m.zip |
Instrument Name: | None |
Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000539 |
Analysis ID: | AN000606 |
Instrument Name: | Agilent 6410 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | QM-005-Xbrdg2mm-CoA-6490.m.zip |