Summary of Study ST000467

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000359. The data can be accessed directly via it's Project DOI: 10.21228/M8NC8M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST000467
Study Title	Metabolomics of Saliva Samples Obtained from Subjects with Diabetes
Study Summary	In this research, were are investigating the metabolic profile changes associated with well- and poorly-controlled type 1 and 2 diabetes and if there are distinct metabolite compounds that may be associated with glycemic control. The PI of the study collected whole unstimulated saliva samples were from subjects with well- and poorly-controlled type 1 and type 2 diabetes. Subjects were selected based on the level of A1C (<7= well-controlled and >7 = poorly controlled). Saliva samples were shipped to RTI RCMRC for a broad spectrum reverse phase metabolomics analysis.
Institute	University of North Carolina
Department	Discovery-Sciences-Technology (DST)
Laboratory	Sumner Lab
Last Name	Sumner
First Name	Susan
Address	Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Email	susan_sumner @unc.edu
Phone	704-250-5066
Submit Date	2016-09-02
Num Groups	4
Total Subjects	40
Raw Data Available	Yes
Raw Data File Type(s)	raw(Waters)
Analysis Type Detail	LC-MS
Release Date	2017-10-03
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000359
Project DOI:	doi: 10.21228/M8NC8M
Project Title:	Metabolomics of Saliva Samples Obtained from Subjects with Diabetes
Project Summary:	Over 20 millions of people suffer from diabetes and a quarter of them have no knowledge of their diabetic condition. Even though, monitoring blood sugar is commonly used to diagnose and monitor the progression of diabetes, it is not the most reliable tool. Measuring serum hemoglobin A1C is the most reliable tool in diagnosing and monitoring diabetes but requires laboratory support and professional interpretation. In this research, were are investigating the metabolic profile changes associated with well- and poorly-controlled type 1 and 2 diabetes and if there are distinct metabolite compounds that may be associated with glycemic control.
Institute:	University of North Carolina at Chapel Hill
Department:	Department of Prosthodontics
Last Name:	Bencharit
First Name:	Sompop
Address:	342 Brauer Hall, Department of Prosthodontics, Chapel Hill, NC 27599-7450
Email:	Sompop_Bencharit@unc.edu
Phone:	919-537-3861

Subject:

Subject ID:	SU000488
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	DM Type	A1C Category
SA023614	S_140	1	Poorly Controlled
SA023615	S_5	1	Poorly Controlled
SA023616	S_95	1	Poorly Controlled
SA023617	S_103	1	Poorly Controlled
SA023618	S_85	1	Poorly Controlled
SA023619	S_137	1	Poorly Controlled
SA023620	S_67	1	Poorly Controlled
SA023621	S_87	1	Poorly Controlled
SA023622	S_47	1	Poorly Controlled
SA023623	S_75	1	Poorly Controlled
SA023624	S_9	1	Well Controlled
SA023625	S_1	1	Well Controlled
SA023626	S_62	1	Well Controlled
SA023627	S_109	1	Well Controlled
SA023628	S_110	1	Well Controlled
SA023629	S_22	1	Well Controlled
SA023630	S_94	1	Well Controlled
SA023631	S_46	1	Well Controlled
SA023632	S_44	1	Well Controlled
SA023633	S_51	1	Well Controlled
SA023634	S_45	2	Poorly Controlled
SA023635	S_32	2	Poorly Controlled
SA023636	S_151	2	Poorly Controlled
SA023637	S_96	2	Poorly Controlled
SA023638	S_21	2	Poorly Controlled
SA023639	S_126	2	Poorly Controlled
SA023640	S_65	2	Poorly Controlled
SA023641	S_27	2	Poorly Controlled
SA023642	S_76	2	Poorly Controlled
SA023643	S_107	2	Poorly Controlled
SA023644	S_114	2	Well Controlled
SA023645	S_73	2	Well Controlled
SA023646	S_92	2	Well Controlled
SA023647	S_153	2	Well Controlled
SA023648	S_133	2	Well Controlled
SA023649	S_54	2	Well Controlled
SA023650	S_108	2	Well Controlled
SA023651	S_130	2	Well Controlled
SA023652	S_93	2	Well Controlled
SA023653	S_106	2	Well Controlled
SA023604	TP_1	-	Total Pool_1
SA023605	TP_10	-	Total Pool_10
SA023606	TP_2	-	Total Pool_2
SA023607	TP_3	-	Total Pool_3
SA023608	TP_4	-	Total Pool_4
SA023609	TP_5	-	Total Pool_5
SA023610	TP_6	-	Total Pool_6
SA023611	TP_7	-	Total Pool_7
SA023612	TP_8	-	Total Pool_8
SA023613	TP_9	-	Total Pool_9

Showing results 1 to 50 of 50

Showing results 1 to 50 of 50

Collection:

Collection ID:	CO000482
Collection Summary:	Saliva samples were collected via standard operating procedures.
Sample Type:	Saliva

Treatment:

Treatment ID:	TR000502
Treatment Summary:	Saliva samples are from subjects with Type I and II diabetes. No treatment was given.

Sample Preparation:

Sampleprep ID:	SP000495
Sampleprep Summary:	Study Samples and Whole Study Pooled QC Aliquots Preparation: Thawed aqueous saliva samples on ice. Samples were then vortexed and centrifuged at room temperature and at 16,000 rcf for four minutes. A 100 µL aliquot of each experimental sample was transferred to a labeled 2.0 mL Lo-Bind Eppendorf tube to make the study sample aliquots. Whole study pooled QC samples were created by combining 100 µL aliquot from each of the study samples into a 2 mL Lo-Bind eppendorf tube. This QC pooled sample was then vortexed for 30 seconds. Then, ten whole study pooled QC samples of 100 µL each were created. Sample Extraction To all samples (study and whole study pooled QC), 500 µL of Protein Precipitation Buffer with internal standard (0.0125 mg/mL of Tryptophan-d5 in methanol) was added to each tube and vortexed for 4 min at room temperature and at 5,000 rpm. The samples were then centrifuged at room temperature and at 16,000 rcf for 4 min. A 450 µL aliquot of the supernatant from each sample was transferred into pre-labeled 2.0 mL LoBind eppendorf tube and stored at -80 °C for one hour followed by a drying step on a lyophilizer. The residue was reconstituted in 100 µL of reconstitution buffer (95:5 Water:Methanol, v/v) and mixed on a multiple tube vortexer for 10 min at 5,000 rpm. Then, the samples were centrifuged at room temperature for 4 min at 16,000 rcf, and the supernatants were transferred to autosampler vials for data acquisition.

Sampleprep ID:

SP000495

Sampleprep Summary:

Study Samples and Whole Study Pooled QC Aliquots Preparation: Thawed aqueous saliva samples on ice. Samples were then vortexed and centrifuged at room temperature and at 16,000 rcf for four minutes. A 100 µL aliquot of each experimental sample was transferred to a labeled 2.0 mL Lo-Bind Eppendorf tube to make the study sample aliquots. Whole study pooled QC samples were created by combining 100 µL aliquot from each of the study samples into a 2 mL Lo-Bind eppendorf tube. This QC pooled sample was then vortexed for 30 seconds. Then, ten whole study pooled QC samples of 100 µL each were created. Sample Extraction To all samples (study and whole study pooled QC), 500 µL of Protein Precipitation Buffer with internal standard (0.0125 mg/mL of Tryptophan-d5 in methanol) was added to each tube and vortexed for 4 min at room temperature and at 5,000 rpm. The samples were then centrifuged at room temperature and at 16,000 rcf for 4 min. A 450 µL aliquot of the supernatant from each sample was transferred into pre-labeled 2.0 mL LoBind eppendorf tube and stored at -80 °C for one hour followed by a drying step on a lyophilizer. The residue was reconstituted in 100 µL of reconstitution buffer (95:5 Water:Methanol, v/v) and mixed on a multiple tube vortexer for 10 min at 5,000 rpm. Then, the samples were centrifuged at room temperature for 4 min at 16,000 rcf, and the supernatants were transferred to autosampler vials for data acquisition.

Combined analysis:

Analysis ID	AN000730
Analysis type	MS
Chromatography type	Reversed phase
Chromatography system	Waters Acquity
Column	Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type	ESI
MS instrument type	QTOF
MS instrument name	Waters
Ion Mode	POSITIVE
Units	m/z

Chromatography:

Chromatography ID:	CH000523
Instrument Name:	Waters Acquity
Column Name:	Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Chromatography Type:	Reversed phase

MS:

MS ID:	MS000647
Analysis ID:	AN000730
Instrument Name:	Waters
Instrument Type:	QTOF
MS Type:	ESI
Ion Mode:	POSITIVE