Summary of Study ST000476

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000320. The data can be accessed directly via it's Project DOI: 10.21228/M8MP4W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000476
Study TitleMetabolomic analysis of oxytocin effects on social deficits in mice (part IV)
Study SummaryThis metabolomics pilot study evaluated serum from mice treated with oxytocin and vehicle to understand how these factors influence the metabotype.
Institute
University of North Carolina
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2016-08-31
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-10-03
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8MP4W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000320
Project DOI:doi: 10.21228/M8MP4W
Project Title:Metabolomic analysis of oxytocin effects on social deficits in mice
Project Summary:The goal of this study was to determine the effects of the neuropeptide oxytocin (OT) on metabolomic profiles, using a mouse model of autism-like behavior, the BALB/cByJ inbred strain. We have previously reported that subchronic treatment with OT can lead to persistent reversal of social deficits in BALB/cByJ and other models of autism spectrum disorder (ASD). In this study, mice were given a subchronic regimen with either vehicle or OT, and then evaluated for social approach. At the end of the study, brain and blood were collected for metabolomic analysis. In addition, fecal samples were taken at different time points during the treatment and testing regimen. The results from this project could elucidate mechanisms underlying the prosocial effects of oxytocin, and identify new targets for the development of highly specific oxytocin-related drugs.
Institute:University of North Carolina at Chapel Hill
Department:Department of Psychiatry
Last Name:Moy
First Name:Sheryl
Address:CB# 7146, Chapel Hill, NC 277599
Email:ssmoy@med.unc.edu
Phone:919-966-3082

Subject:

Subject ID:SU000497
Subject Type:Murine
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Murine; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA024348S_8Oxytocin
SA024349S_3Oxytocin
SA024350S_1Oxytocin
SA024351S_19Oxytocin
SA024352S_5Oxytocin
SA024353S_17Oxytocin
SA024354S_15Oxytocin
SA024355S_6Oxytocin
SA024356S_10Oxytocin
SA024357S_14Oxytocin
SA024358S_12Oxytocin
SA024359S_2Oxytocin
SA024360PS_1Pool
SA024361PS_2Pool
SA024362S_13Veh
SA024363S_7Veh
SA024364S_18Veh
SA024365S_9Veh
SA024366S_4Veh
SA024367S_16Veh
SA024368S_11Veh
SA024369S_20Veh
Showing results 1 to 22 of 22

Collection:

Collection ID:CO000491
Collection Summary:Trunk blood was collected into centrifuge tubes and centrifuged for 15 min at ~1,000 x g at 4o C. Supernatant (serum) was extracted, frozen on dry ice, and stored at -80o C.
Sample Type:Blood

Treatment:

Treatment ID:TR000511
Treatment Summary:BALB/cByJ male mice (4-5 weeks in age; Jackson Laboratory, Bar Harbor, ME) were given four treatments of either vehicle or OT (1.0 mg/kg; IP) across 8 days, with at least 48 hours between each injection. Mice were evaluated in a 3-chamber choice test for social preference 24 hr after the final injection.

Sample Preparation:

Sampleprep ID:SP000504
Sampleprep Summary:30 µL of each serum sample was transferred to a new, pre-labeled 1.5 mL LoBind eppendorf tube to make the individual study samples, and 12 µL was transferred to a new, pre-labeled 1.5 mL LoBind Eppendorf to make the total pool. The total pool was aliquoted into 3 Total Pool QC Samples and 4 Column Equilibrium samples with volumes of 30 µL in 1.5 mL LoBind Eppendorf tubes. 600 uL of Lipid Extraction Solvent (PC(17:0/17:0) 50 µg/mL in 2:1 dichloromethane:methanol) was added to all samples, and samples were vortexed at 4,000 rpm for 2mins on multiple-tube vortexer. 120 µL of H2O was added to each tube and the samples were vortexed at 4,000 rpm for 1min. Samples sat at room temperature for 10 minutes, and were centrifuged at 16,000 rcf for 10 min at 10°C. 240 µL of the lower lipid-rich DCM layer was transferred into new pre-labeled 1.5 mL LoBind Eppendorf tubes, and rubber stoppers were inserted. Samples were placed at -80˚C for 60 mins, and then lyophilized for 19.5 hrs. 240 µL of ACN/IPA/H2O (65:30:5 v/v/v) was added to each sample, and the samples were vortexed at 4,000 rpm for 2mins and centrifuged at 16,000 rcf for 4 min. 225 µL of the supernatant was transferred to pre-labeled autosampler vials and 10 µL was injected into OrbiTrap Velos.

Combined analysis:

Analysis ID AN000741
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Orbitrap
Ion Mode POSITIVE
Units MS

Chromatography:

Chromatography ID:CH000533
Instrument Name:Waters Acquity
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000658
Analysis ID:AN000741
Instrument Name:Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
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