Summary of Study ST000588
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000430. The data can be accessed directly via it's Project DOI: 10.21228/M8GC82 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000588 |
| Study Title | Metabolomics of Saliva in Decompensated Heart Failure |
| Study Summary | The aim of the study was to test weather characteristic differences or changes in metabolic profile exist between exhaled breath condensate (EBC) and saliva of healthy individuals and heart failure patients. Salival NMR profiling was performed. |
| Institute | Mayo Clinic |
| Department | Cardiovascular Diseases |
| Laboratory | Mayo Metabolomics Core |
| Last Name | Johnson |
| First Name | Bruce |
| Address | 200 First Street SW, Rochester, MN 55905 |
| johnson.bruce@mayo.edu | |
| Phone | 507-284-4441 |
| Submit Date | 2017-04-07 |
| Analysis Type Detail | NMR |
| Release Date | 2019-05-15 |
| Release Version | 1 |
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Project:
| Project ID: | PR000430 |
| Project DOI: | doi: 10.21228/M8GC82 |
| Project Title: | Mayo Metabolomics Pilot and Feasibility Award: Metabolomics of Exhaled Breath Condensate in Decompensated Heart Failure |
| Project Summary: | Metabolomics is a large-scale approach to monitoring as many as possible of the compounds involved in cellular processes in a single assay to derive metabolic profiles. Compared with genomics or proteomics, metabolomics reflects changes in phenotype and therefore function. Nearly all the blood volume-cardiac output in humans flows through the lungs at a rate of 5L/min and traverses through capillaries with a very thin blood-gas barrier (approximately 2μm, 600–800 nm; in some places <200 nm) to allow sufficient gas diffusion. However, there is evidence that many other biochemicals from the blood can be found in the exhaled air. Collection of exhaled breath condensate (EBC) is a relatively new, noninvasive method that allows assessment of biochemical profiles that may not only link to airway physiology, but also to other changing processes in the body. The aims of this study are: (1) Working with the Mayo Clinic Metabolomics Resource Core Laboratory to develop the optimal method for performing metabolomics of EBC, (2) Collecting and metabolomic profiling of the EBCs from patients with heart failure; (2) Comparing the profiles of these patients obtained during stable and acute exacerbation periods as well as during recovery from a decompensation; (3) Comparing the profiles of these patients with age and gender matched controls; (4) Correlation of EBC metabolomic profiles with clinical phenotypes. The proposed metabolomics profiles would be obtained from patients involved in IRB approved, ongoing, primarily NIH funded studies focused on understanding pulmonary congestion in heart failure patients. This includes a recently started project capturing patients as they present in the emergency room with decompensated heart failure. Phenotyping for this study includes novel measures of pulmonary capillary recruitment, alveolar-capillary conductance, pulmonary capillary blood volume, thoracic CT imaging combined with in-hospital clinical data, including blood biomarkers such as BNP and catecholamines. Measurements are performed upon admission (within 24hours) and then repeated just prior to dismissal. In addition, we would collect EBC on stable HF patients and in age and gender matched controls healthy controls from our other ongoing studies. The overall aim will be to identify novel patterns or biomarkers for developing a point-of-care test (POC) for tracking disease status in HF patients. |
| Institute: | Mayo Clinic |
| Department: | Cardiovascular Diseases |
| Laboratory: | Mayo Metabolomics Core |
| Last Name: | Johnson |
| First Name: | Bruce |
| Address: | 200 First St. SW, Rochester, Minnesota, 55905, USA |
| Email: | johnson.bruce@mayo.edu |
| Phone: | 507-284-4441 |
Subject:
| Subject ID: | SU000611 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Group |
|---|---|---|
| SA031841 | C22 | Control |
| SA031842 | C23 | Control |
| SA031843 | C10 | Control |
| SA031844 | C20 | Control |
| SA031845 | C24 | Control |
| SA031846 | C21 | Control |
| SA031847 | C19 | Control |
| SA031848 | C11 | Control |
| SA031849 | C15 | Control |
| SA031850 | C14 | Control |
| SA031851 | C16 | Control |
| SA031852 | C18 | Control |
| SA031853 | HF12 | Heart failure |
| SA031854 | HF09 | Heart failure |
| SA031855 | HF17 | Heart failure |
| SA031856 | HF08 | Heart failure |
| SA031857 | HF13 | Heart failure |
| SA031858 | HF01 | Heart failure |
| SA031859 | HF03 | Heart failure |
| SA031860 | HF02 | Heart failure |
| SA031861 | HF04 | Heart failure |
| SA031862 | HF05 | Heart failure |
| SA031863 | HF06 | Heart failure |
| SA031864 | HF07 | Heart failure |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO000605 |
| Collection Summary: | The samples were collected in Dr. Bruce Johnson lab, at the Department of Cardiovascular Diseases, Mayo Clinic. EBC samples were collected using Jaeger EcoScreen cooling unit (-20°C). Patients were breathing through mouth piece while wearing a nose clip during 30 minutes. Saliva was collected from the same patients during last 15 minutes. The samples were frozen immediately after collection. |
| Sample Type: | Saliva |
Treatment:
| Treatment ID: | TR000625 |
| Treatment Summary: | We will focus initially on patients with classic systolic HF who are admitted to St Mary’s Hospital with decompensated disease. These patients are currently being studied to quantify changes in lung water during the “recompensation” diuresis in the hospital and the related changes in alveolar gas conductance and breathing pattern to develop a predictive algorithm for likelihood of readmission, but also to obtain a better understanding of fluid regulation in the lungs. As these subjects are recruited, we will recruit age and sex matched stable HF patients going through similar testing in parallel running studies as well as age and sex matched control subjects. We will target 12 subjects per group and in addition to the EBC collection we will obtain saliva samples and blood at the same time points. |
Sample Preparation:
| Sampleprep ID: | SP000618 |
| Sampleprep Summary: | Saliva: Allow samples to thaw on ice at 4 °C for 30–60 min. Transfer 250 μl aliquots of saliva samples into 1.5ml EP tubes, centrifuge each sample at 13,300g for 10 min. Transfer 200μl of supernatant of saliva samples into 1.5 ml EP tubes, add 150 μl of phosphate buffer and 150 μl of 4mM TSP-d4 solution in D2O. Vortex for 20 s. Transfer the solution to 5 mm NMR tube. |
Analysis:
| Analysis ID: | AN000903 |
| Analysis Type: | NMR |
| Num Factors: | 2 |
| Num Metabolites: | 48 |
| Units: | uM |
NMR:
| NMR ID: | NM000101 |
| Analysis ID: | AN000903 |
| Instrument Name: | Bruker 600 MHz Avance III HD spectrometer |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 600 MHz |
