Summary of Study ST000636

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000457. The data can be accessed directly via it's Project DOI: 10.21228/M8060Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000636
Study TitleNeuromodulator Metabolites of Dietary Salt Effects on Blood Pressure in Human Urine from DASH2 Clinical Trial (part IX)
Study SummaryThe objective of the study is to identify changes of urinary metabolite profiles associated with different responses to blood pressure to salt. Subjects are derived from The Dietary approaches to stop hypertension (DASH) diet, Sodium Intake and Blood Pressure Trial (Sacks FM et al PMID: 11136953,N Engl J Med. 2001).We choose two groups subjects who meet the following conditions(the two groups are separately named A and B). We chose subjects on the Control diet .These subjects meet the blood pressure criteria described below:Group A subjects conditions: 1) On Control diet. 2) Normotensive subjects: systolic blood pressure from the low sodium visit is less than 140 and the diastolic blood pressure from low sodium visit is less than 90; 3) For group A: Either the systolic blood pressure from the high sodium visit was greater than 10 mmHg higher than the systolic blood pressure from the low sodium visit, or the diastolic blood pressure from the high sodium visit was greater than 10 mmHg higher than the diastolic blood pressure from the low sodium visit; 3) For group B: The systolic blood pressure from the high sodium visit is within 5 mmHg (i.e. +/- 5) from the systolic blood pressure from the low sodium visit, and the diastolic blood pressure from the high sodium visit is within 5 mmHg from the diastolic blood pressure from the low sodium visit. Use gas chromatography/mass spectrometry (GC/MS) analysis, and liquid chromatography/mass spectrometry (LC/MS)analysis to find the differences of metabolic profiles between the high sodium level and the low sodium level, and compare the metabolic profiles of A with the metabolic profiles of B at the low and high sodium level.
Institute
Mayo Clinic
Last NameLiang
First NameMingyu
AddressMedical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226
Emailmliang@mcw.edu
Phone414-955-8539
Submit Date2017-06-23
Analysis Type DetailLC-MS
Release Date2019-07-17
Release Version1
Mingyu Liang Mingyu Liang
https://dx.doi.org/10.21228/M8060Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000457
Project DOI:doi: 10.21228/M8060Z
Project Title:Metabolomic Mechanisms of Dietary Salt Effects on Blood Pressure
Project Summary:Enhanced sensitivity of blood pressure to salt intake is observed in approximately 50% of hypertensive patients, reaching 75% in African American hypertensive patients. We recently discovered a novel role of abnormal cellular intermediary metabolism in hypertension in the Dahl salt-sensitive (SS) rat, the most commonly used polygenic, hereditary model of human salt-sensitive hypertension. We propose to test the hypothesis that blood pressure sensitivity to dietary salt intake in human is associated with metabolite changes in the urine. Leveraging the expertise and resources at the Mayo Clinic Metabolomics Resources Core, we propose to perform targeted LC/MS analysis and NMR spectra generation in urine samples obtained from a subset of subjects from the Dietary Approaches to Stop Hypertension – Sodium (DASH2) clinical trial and kidney tissue extract and urine samples from SS rats and a newly generated transgenic rat that overexpresses fumarase (SS.Fh1+). The study will be the first to systematically characterize urinary metabolite profiles associated with blood pressure response to salt in humans. The study is anticipated to generate new insight into the mechanisms (particularly renal mechanisms) underlying salt-sensitive hypertension. Findings of the proposed study could lead to an expanded clinical study as well as mechanistic studies in animal models.
Institute:Mayo Clinic
Last Name:Liang
First Name:Mingyu
Address:Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226
Email:mliang@mcw.edu
Phone:414-955-8539

Subject:

Subject ID:SU000659
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sodium level grouping
SA035785ms5957-135H A
SA035786ms5957-156H A
SA035787ms5957-116H A
SA035788ms5957-56H A
SA035789ms5957-61H A
SA035790ms5957-44H A
SA035791ms5957-115H A
SA035792ms5957-157H A
SA035793ms5957-32H A
SA035794ms5957-31H A
SA035795ms5957-159H A
SA035796ms5957-37H A
SA035797ms5957-158H A
SA035798ms5957-62H A
SA035799ms5957-63H A
SA035800ms5957-86H A
SA035801ms5957-104H A
SA035802ms5957-90H A
SA035803ms5957-92H A
SA035804ms5957-98H A
SA035805ms5957-79H A
SA035806ms5957-107H A
SA035807ms5957-67H A
SA035808ms5957-113H A
SA035809ms5957-70H A
SA035810ms5957-71H A
SA035811ms5957-73H A
SA035812ms5957-160H A
SA035813ms5957-121H A
SA035814ms5957-11H A
SA035815ms5957-129H A
SA035816ms5957-18H A
SA035817ms5957-9H A
SA035818ms5957-8H A
SA035819ms5957-4H A
SA035820ms5957-131H A
SA035821ms5957-20H A
SA035822ms5957-15H A
SA035823ms5957-26H A
SA035824ms5957-25H A
SA035825ms5957-161H A
SA035826ms5957-164H B
SA035827ms5957-108H B
SA035828ms5957-74H B
SA035829ms5957-138H B
SA035830ms5957-163H B
SA035831ms5957-133H B
SA035832ms5957-40H B
SA035833ms5957-68H B
SA035834ms5957-123H B
SA035835ms5957-36H B
SA035836ms5957-124H B
SA035837ms5957-88H B
SA035838ms5957-166H B
SA035839ms5957-93H B
SA035840ms5957-134H B
SA035841ms5957-165H B
SA035842ms5957-103H B
SA035843ms5957-81H B
SA035844ms5957-27H B
SA035845ms5957-83H B
SA035846ms5957-144H B
SA035847ms5957-130H B
SA035848ms5957-13H B
SA035849ms5957-64H B
SA035850ms5957-118H B
SA035851ms5957-43H B
SA035852ms5957-117H B
SA035853ms5957-136H B
SA035854ms5957-50H B
SA035855ms5957-162H B
SA035856ms5957-127H B
SA035857ms5957-128H B
SA035858ms5957-45H B
SA035859ms5957-126H B
SA035860ms5957-41H B
SA035861ms5957-16H B
SA035862ms5957-125H B
SA035863ms5957-42H B
SA035864ms5957-60H B
SA035865ms5957-59H B
SA035866ms5957-58H B
SA035867ms5957-19H B
SA035868ms5957101L A
SA035869ms5957-100L A
SA035870ms5957-99L A
SA035871ms5957-105L A
SA035872ms5957-114L A
SA035873ms5957-112L A
SA035874ms5957-139L A
SA035875ms5957-106L A
SA035876ms5957-122L A
SA035877ms5957-97L A
SA035878ms5957-140L A
SA035879ms5957-66L A
SA035880ms5957-21L A
SA035881ms5957-52L A
SA035882ms5957-17L A
SA035883ms5957-14L A
SA035884ms5957-150L A
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Collection:

Collection ID:CO000653
Collection Summary:The urine samples we have were 24-hour collections occurring in the fourth week on either the low- or the high-sodium intake level. In total, 120 human samples (60 subjects, a low-sodium sample and a high-sodium sample for each subject) will be analyzed.
Sample Type:Urine

Treatment:

Treatment ID:TR000673
Treatment Summary:We will analyze urine specimens collected during the Dietary Approaches to Stop Hypertension – Sodium Study (DASH-Sodium or DASH2) clinical trial. More than 400 participants in the study were divided into two groups. One followed a common Western diet, while the other group followed the “healthier” DASH diet. Then each group had three 30 day periods, in random orders, that corresponded to high, intermediate, or low sodium intakes while eating their designated diet. The participants had all of their meals prepared and provided for them during the entire study. It was found that reducing sodium intake significantly reduced blood pressure especially when on the common Western diet. We have obtained urine samples and phenotypic data for a subset of the participants of the DASH2 trial form the NHLBI biospecimen and data repository BioLINCC. We selected two sub-groups of participants on the Western diet. Group A (n=31) exhibited large (>12 mmHg) differences in systolic or diastolic pressure between low- and high-sodium intakes (“salt-sensitive” group). Group B (n=29) showed similar blood pressure (differences <5 mmHg for both systolic and diastolic pressures) between low- and high-sodium intakes (“saltinsensitive” group).

Sample Preparation:

Sampleprep ID:SP000666
Sampleprep Summary:2 drops of 6 N HCl was added in every 8 ml urine samples when the 24 hour urine was collected. All urine samples were stored in -80℃ freezer.

Combined analysis:

Analysis ID AN000968
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantum Ultra
Ion Mode POSITIVE
Units uM

Chromatography:

Chromatography ID:CH000693
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000863
Analysis ID:AN000968
Instrument Name:Thermo Quantum Ultra
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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