Summary of Study ST000670

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000473. The data can be accessed directly via it's Project DOI: 10.21228/M8X61Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000670
Study TitleExposure to high fat diets and bisphenol
Study TypeMS analysis
Study SummaryWild type (a/a) agouti mouse dams were randomized to one of three diets (control, mediterranean, western) two weeks prior to pairing with an agouti (Avy/a) sire. Diet exposure continued through pregnancy and lactation. All pups were weaned onto the control diet and then followed for metabolic phenotyping measures to 10 months of age. Comprehensive phenotyping (body composition, CLAMS, blood draw) was completed at 2, 4, and 8 months, with an OGTT at 8 months. Weekly weights were also recorded. The study examines whether prenatal dietary exposure to high fat diets (HFD) and bisphenol a (BPA, those groups will not be tested in this pilot) impacts metabolic programming in offspring as measured by hepatic steatosis, serum hormone levels, and epigenetic changes in hepatic lipid metabolism genes.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups8
Total Subjects6
Study CommentsShotgun lipidomics will add insight into the potentially changing lipid composition of membranes in offspring following different prenatal diet exposures as a means of assessing risk of steatosis and metabolic changes.
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8X61Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000473
Project DOI:doi: 10.21228/M8X61Q
Project Title:Prenatal exposure to BPA and HFDs
Project Type:MS analysis
Project Summary:Prenatal exposure to BPA and HFDs are studied to determine impact on metabolic syndrome and non-alcoholic fatty liver disease outcomes in mouse pups from birth through 10 months
Institute:University of Michigan
Department:Environmental Health Sciences
Laboratory:Dolinoy Lab
Last Name:Dolinoy
First Name:Dana
Address:Ann Arbor, MI
Email:ddolinoy@umich.edu
Phone:734-647-3155

Subject:

Subject ID:SU000693
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Diet Sex
SA038501S00019954Control + BPA Chow F
SA038502S00019958Control + BPA Chow F
SA038503S00019962Control + BPA Chow F
SA038504S00019952Control + BPA Chow F
SA038505S00019960Control + BPA Chow F
SA038506S00019956Control + BPA Chow F
SA038507S00019957Control + BPA Chow M
SA038508S00019955Control + BPA Chow M
SA038509S00019959Control + BPA Chow M
SA038510S00019953Control + BPA Chow M
SA038511S00019963Control + BPA Chow M
SA038512S00019961Control + BPA Chow M
SA038513S00019930Control Chow F
SA038514S00019932Control Chow F
SA038515S00019928Control Chow F
SA038516S00019927Control Chow M
SA038517S00019931Control Chow M
SA038518S00019929Control Chow M
SA038519S00019925Control Chow M
SA038520S00019926Control Chow M
SA038521S00019933Control Chow M
SA038522S00019968Mediterranean + BPA Chow F
SA038523S00019974Mediterranean + BPA Chow F
SA038524S00019972Mediterranean + BPA Chow F
SA038525S00019970Mediterranean + BPA Chow F
SA038526S00019966Mediterranean + BPA Chow F
SA038527S00019964Mediterranean + BPA Chow F
SA038528S00019969Mediterranean + BPA Chow M
SA038529S00019971Mediterranean + BPA Chow M
SA038530S00019973Mediterranean + BPA Chow M
SA038531S00019975Mediterranean + BPA Chow M
SA038532S00019965Mediterranean + BPA Chow M
SA038533S00019967Mediterranean + BPA Chow M
SA038534S00019941Mediterranean HFD Chow F
SA038535S00019937Mediterranean HFD Chow F
SA038536S00019939Mediterranean HFD Chow F
SA038537S00019936Mediterranean HFD Chow M
SA038538S00019938Mediterranean HFD Chow M
SA038539S00019942Mediterranean HFD Chow M
SA038540S00019940Mediterranean HFD Chow M
SA038541S00019934Mediterranean HFD Chow M
SA038542S00019935Mediterranean HFD Chow M
SA038543S00019986Western + BPA Chow F
SA038544S00019976Western + BPA Chow F
SA038545S00019978Western + BPA Chow F
SA038546S00019982Western + BPA Chow F
SA038547S00019980Western + BPA Chow F
SA038548S00019984Western + BPA Chow F
SA038549S00019985Western + BPA Chow M
SA038550S00019979Western + BPA Chow M
SA038551S00019977Western + BPA Chow M
SA038552S00019983Western + BPA Chow M
SA038553S00019987Western + BPA Chow M
SA038554S00019981Western + BPA Chow M
SA038555S00019950Western HFD Chow F
SA038556S00019948Western HFD Chow F
SA038557S00019946Western HFD Chow F
SA038558S00019945Western HFD Chow M
SA038559S00019951Western HFD Chow M
SA038560S00019949Western HFD Chow M
SA038561S00019947Western HFD Chow M
SA038562S00019944Western HFD Chow M
SA038563S00019943Western HFD Chow M
Showing results 1 to 63 of 63

Collection:

Collection ID:CO000687
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000707
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000700
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001026 AN001027
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000733
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS000921
Analysis ID:AN001026
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS000922
Analysis ID:AN001027
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
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