Summary of Study ST000753
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000543. The data can be accessed directly via it's Project DOI: 10.21228/M8GH3J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000753 |
Study Title | Comparison of the metabolome and lipidome of wild type and mdx/mTR mice |
Study Type | MS analysis |
Study Summary | There are two groups of mice in the study, wild type mice (WT) and mdx/mTR mice (mdx or mdx/mTR) that are a model of musclar dystrophy. We collected the tibialis anterior muscles from these mice (N=6 in each group), and would like to perform TCA-plus analysis and shotgun lipidomics analysis on the mice, and compare across the two groups. |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2017-06-19 |
Num Groups | 2 |
Total Subjects | 12 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2017-12-06 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000543 |
Project DOI: | doi: 10.21228/M8GH3J |
Project Title: | Comparison of the metabolome and lipidome of wild type and mdx/mTR mice |
Project Type: | MS analysis |
Project Summary: | A comparison of the metabolome and lipidome of wild type and mdx/mTR mice |
Institute: | University of Michigan |
Department: | Orthopaedic Surgery |
Laboratory: | Mendias Lab |
Last Name: | Mendias |
First Name: | Christopher |
Address: | Ann Arbor, MI |
Email: | cmendias@umich.edu |
Phone: | 734-764-3250 |
Subject:
Subject ID: | SU000776 |
Subject Type: | Mouse |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Genotype |
---|---|---|
SA041490 | S00025567 | mdx/mTR |
SA041491 | S00025566 | mdx/mTR |
SA041492 | S00025562 | mdx/mTR |
SA041493 | S00025563 | mdx/mTR |
SA041494 | S00025564 | mdx/mTR |
SA041495 | S00025565 | mdx/mTR |
SA041484 | S00025556 | WT |
SA041485 | S00025561 | WT |
SA041486 | S00025560 | WT |
SA041487 | S00025557 | WT |
SA041488 | S00025558 | WT |
SA041489 | S00025559 | WT |
Showing results 1 to 12 of 12 |
Collection:
Collection ID: | CO000770 |
Collection Summary: | - |
Sample Type: | Muscle |
Treatment:
Treatment ID: | TR000790 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP000783 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
Analysis ID | AN001181 | AN001182 | AN001183 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | HILIC |
Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 | Agilent |
Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Phenomenex Luna NH2 (150 x 1mm,3um) |
MS Type | ESI | ESI | ESI |
MS instrument type | Triple TOF | Triple TOF | QTOF |
MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF | Agilent 6520B QTOF |
Ion Mode | NEGATIVE | POSITIVE | NEGATIVE |
Units | peak area normalized | peak area normalized | counts |
Chromatography:
Chromatography ID: | CH000829 |
Instrument Name: | Shimadzu CTO-20A Nexera X2 |
Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
Column Temperature: | 55 |
Flow Rate: | 0.400uL/min |
Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000830 |
Instrument Name: | Agilent |
Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
Flow Rate: | 0.075 mL/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |
MS:
MS ID: | MS001076 |
Analysis ID: | AN001181 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
MS ID: | MS001077 |
Analysis ID: | AN001182 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
MS ID: | MS001078 |
Analysis ID: | AN001183 |
Instrument Name: | Agilent 6520B QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | A011_-_Glycolysis-TCA-nucleotides.pdf |