Summary of Study ST000753
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000543. The data can be accessed directly via it's Project DOI: 10.21228/M8GH3J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000753 |
| Study Title | Comparison of the metabolome and lipidome of wild type and mdx/mTR mice |
| Study Type | MS analysis |
| Study Summary | There are two groups of mice in the study, wild type mice (WT) and mdx/mTR mice (mdx or mdx/mTR) that are a model of musclar dystrophy. We collected the tibialis anterior muscles from these mice (N=6 in each group), and would like to perform TCA-plus analysis and shotgun lipidomics analysis on the mice, and compare across the two groups. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 2 |
| Total Subjects | 12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000543 |
| Project DOI: | doi: 10.21228/M8GH3J |
| Project Title: | Comparison of the metabolome and lipidome of wild type and mdx/mTR mice |
| Project Type: | MS analysis |
| Project Summary: | A comparison of the metabolome and lipidome of wild type and mdx/mTR mice |
| Institute: | University of Michigan |
| Department: | Orthopaedic Surgery |
| Laboratory: | Mendias Lab |
| Last Name: | Mendias |
| First Name: | Christopher |
| Address: | Ann Arbor, MI |
| Email: | cmendias@umich.edu |
| Phone: | 734-764-3250 |
Subject:
| Subject ID: | SU000776 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA041490 | S00025567 | mdx/mTR |
| SA041491 | S00025566 | mdx/mTR |
| SA041492 | S00025562 | mdx/mTR |
| SA041493 | S00025563 | mdx/mTR |
| SA041494 | S00025564 | mdx/mTR |
| SA041495 | S00025565 | mdx/mTR |
| SA041484 | S00025556 | WT |
| SA041485 | S00025561 | WT |
| SA041486 | S00025560 | WT |
| SA041487 | S00025557 | WT |
| SA041488 | S00025558 | WT |
| SA041489 | S00025559 | WT |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO000770 |
| Collection Summary: | - |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR000790 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000783 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN001181 | AN001182 | AN001183 |
|---|---|---|---|
| Chromatography ID | CH000829 | CH000829 | CH000830 |
| MS ID | MS001076 | MS001077 | MS001078 |
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC |
| Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF | QTOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF | Agilent 6520B QTOF |
| Ion Mode | NEGATIVE | POSITIVE | NEGATIVE |
| Units | peak area normalized | peak area normalized | counts |
Chromatography:
| Chromatography ID: | CH000829 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000830 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Flow Rate: | 0.075 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001076 |
| Analysis ID: | AN001181 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS001077 |
| Analysis ID: | AN001182 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS001078 |
| Analysis ID: | AN001183 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A011_-_Glycolysis-TCA-nucleotides.pdf |
