Summary of Study ST000841
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000599. The data can be accessed directly via it's Project DOI: 10.21228/M87Q3F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000841 |
| Study Title | Metabolomics of muscle in insulin sensitive and resistant obese indivduals. |
| Study Type | MS analysis |
| Study Summary | OC samples: muscle biopsy samples collected from insulin sensitive / insulin resistant obese indivduals before undergoing a hyperinsulinemic-euglycemic clamp. Subjects were previously categorized for the rate of fatty acid release from adipose tissue into the bloodstream as a measure of insuline resistance. Low-FA, Med-FA, and High-FA stand for low, medium, and high rate of release. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-08-02 |
| Num Groups | 4 |
| Total Subjects | 28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000599 |
| Project DOI: | doi: 10.21228/M87Q3F |
| Project Title: | Prelim data for NIH exercise consortium |
| Project Type: | MS analysis |
| Project Summary: | Plasma samples before and after exercise training in obese adults |
| Institute: | University of Michigan |
| Department: | Kinesiology |
| Laboratory: | Horowitz Lab |
| Last Name: | Horowitz |
| First Name: | Jeff |
| Address: | Ann Arbor, MI |
| Email: | jeffhoro@umich.edu |
| Phone: | 734-647-3354 |
Subject:
| Subject ID: | SU000868 |
| Subject Type: | HUMAN |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Subject group |
|---|---|---|
| SA046693 | S00020440 | HIGH-FA |
| SA046694 | S00020450 | HIGH-FA |
| SA046695 | S00020451 | HIGH-FA |
| SA046696 | S00020449 | HIGH-FA |
| SA046697 | S00020452 | HIGH-FA |
| SA046698 | S00020447 | HIGH-FA |
| SA046699 | S00020445 | HIGH-FA |
| SA046700 | S00020448 | HIGH-FA |
| SA046701 | S00020439 | HIGH-FA |
| SA046702 | S00020427 | LOW-FA |
| SA046703 | S00020442 | LOW-FA |
| SA046704 | S00020436 | LOW-FA |
| SA046705 | S00020430 | LOW-FA |
| SA046706 | S00020428 | LOW-FA |
| SA046707 | S00020431 | LOW-FA |
| SA046708 | S00020433 | LOW-FA |
| SA046709 | S00020434 | LOW-FA |
| SA046710 | S00020446 | MID-FA |
| SA046711 | S00020441 | MID-FA |
| SA046712 | S00020432 | MID-FA |
| SA046713 | S00020429 | MID-FA |
| SA046714 | S00020444 | MID-FA |
| SA046715 | S00020435 | MID-FA |
| SA046716 | S00020437 | MID-FA |
| SA046717 | S00020438 | MID-FA |
| SA046718 | S00020443 | MID-FA |
| SA046719 | S00020453 | N/A |
| SA046720 | S00020454 | N/A |
| Showing results 1 to 28 of 28 |
Collection:
| Collection ID: | CO000862 |
| Collection Summary: | - |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR000882 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000875 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Chromatography:
| Chromatography ID: | CH000944 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000945 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000946 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Rate: | 0.250 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000947 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Flow Rate: | 0.075 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
Analysis:
| Analysis ID: | AN001349 |
| Analysis Type: | MS |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| Instrument Name: | 6530 Q-TOF LC/MS (Agilent) |
| Chromatography ID: | CH000944 |
| Num Factors: | 4 |
| Num Metabolites: | 81 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST000841_AN001349_Results.txt |
| Units: | counts |
| Analysis ID: | AN001350 |
| Analysis Type: | MS |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| Instrument Name: | 6530 Q-TOF LC/MS (Agilent) |
| Chromatography ID: | CH000944 |
| Num Factors: | 4 |
| Num Metabolites: | 77 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST000841_AN001350_Results.txt |
| Units: | Counts |
| Analysis ID: | AN001351 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000945 |
| Num Factors: | 4 |
| Num Metabolites: | 156 |
| Rt Units: | Minutes |
| Units: | counts norm |
| Analysis ID: | AN001352 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000945 |
| Num Factors: | 4 |
| Num Metabolites: | 231 |
| Rt Units: | Minutes |
| Units: | counts norm |
| Analysis ID: | AN001353 |
| Analysis Type: | MS |
| Analysis Protocol File: | A008_-_Acyl_Carnitines.pdf |
| Instrument Name: | 6490A QQQ LC/MS (Agilent) |
| Chromatography ID: | CH000946 |
| Num Factors: | 4 |
| Num Metabolites: | 27 |
| Units: | pmol/mg |
| Analysis ID: | AN001354 |
| Analysis Type: | MS |
| Analysis Protocol File: | A011_-_Glycolysis-TCA-nucleotides.pdf |
| Instrument Name: | 6520B Q-TOF LC/MS (Agilent) |
| Chromatography ID: | CH000947 |
| Num Factors: | 4 |
| Num Metabolites: | 7 |
| Units: | counts |
| Analysis ID: | AN001355 |
| Analysis Type: | MS |
| Analysis Protocol File: | A011_-_Glycolysis-TCA-nucleotides.pdf |
| Instrument Name: | 6520B Q-TOF LC/MS (Agilent) |
| Chromatography ID: | CH000947 |
| Num Factors: | 4 |
| Num Metabolites: | 23 |
| Units: | ug/100 mg |
