Summary of Study ST000921

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000637. The data can be accessed directly via it's Project DOI: 10.21228/M8B96C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000921
Study TitleKarenia brevis allelopathy compromises the lipidome, membrane integrity, and photosynthetic efficiency of competitors
Study TypeUntargeted lipidomics
Study SummaryAllelopathy, or the release of compounds that inhibit competitors, is a form of interference competition that is common among bloom-forming phytoplankton. Allelopathy is hypothesized to play a role in bloom propagation and maintenance and is well established in the red tide dinoflagellate Karenia brevis. K. brevis typically suppresses competitor growth through unknown mechanisms over the course of many days. When we investigated the effects of allelopathy on the lipidomes of two competing phytoplankton, Asterionellopsis glacialis and Thalassiosira pseudonana using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS)- based metabolomics, we found that the lipidomes of both species were significantly altered, however A. glacialis maintained a more robust response whereas T. pseudonana saw significant alterations in fatty acid synthesis, cell membrane integrity, and a decrease in photosynthetic efficiency. Membrane- associated lipids were significantly suppressed for T. pseudonana exposed to allelopathy to the point of permeabilizing the cell membrane of living cells. The dominant mechanisms of K. brevis allelopathy appear to target lipid biosynthesis affecting multiple physiological pathways suggesting that exuded compounds have the ability to significantly alter competitor physiology and give K. brevis a competitive edge over sensitive species.
Institute
Georgia Institute of Technology
DepartmentChemistry
LaboratoryFernández
Last NameHogan
First NameScott
Address901 Atlantic Drive, Atlanta, GA, 30332, USA
Emailsrjhogan@gatech.edu
Phone2156924657
Submit Date2018-01-19
Num Groups4
Total Subjects51
Raw Data AvailableNo
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2018-08-27
Release Version1
Scott Hogan Scott Hogan
https://dx.doi.org/10.21228/M8B96C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000637
Project DOI:doi: 10.21228/M8B96C
Project Title:Karenia brevis allelopathy compromises the lipidome, membrane integrity, and photosynthetic efficiency of competitors
Project Type:Untargeted Lipidomics
Project Summary:Comparing effects on lipidome of phytoplankton competitors based on exposure to K. Brevis
Institute:Georgia Institute of Technology
Department:Chemistry
Laboratory:Fernández
Last Name:Hogan
First Name:Scott
Address:901 Atlantic Drive, Atlanta, GA, 30332, USA
Email:srjhogan@gatech.edu
Phone:2156924657

Subject:

Subject ID:SU000959
Subject Type:Plankton
Subject Species:Thalassiosira pseudonana;Asterionellopsis glacialis
Taxonomy ID:35128;33640
Species Group:Plankton

Factors:

Subject type: Plankton; Subject species: Thalassiosira pseudonana;Asterionellopsis glacialis (Factor headings shown in green)

mb_sample_id local_sample_id Class
SA054646TpC6Control
SA054647TpC11Control
SA054648TpC4Control
SA054649TpC12Control
SA054650TpC9Control
SA054651TpC15Control
SA054652TpC3Control
SA054653AgC2Control
SA054654TpC2Control
SA054655TpC5Control
SA054656TpC10Control
SA054657TpC8Control
SA054658TpC13Control
SA054659TpC7Control
SA054660AgC3Control
SA054661AgC5Control
SA054662AgC1Control
SA054663AgC6Control
SA054664AgC11Control
SA054665AgC4Control
SA054666AgC8Control
SA054667AgC12Control
SA054668AgC7Control
SA054669AgC9Control
SA054670AgC15Control
SA054671AgC10Control
SA054672TpT4treatment
SA054673TpT3treatment
SA054674TpT6treatment
SA054675TpT15treatment
SA054676TpT11treatment
SA054677TpT7treatment
SA054678TpT8treatment
SA054679TpT13treatment
SA054680TpT10treatment
SA054681TpT2treatment
SA054682TpT9treatment
SA054683TpT5treatment
SA054684AgT1treatment
SA054685AgT7treatment
SA054686AgT10treatment
SA054687AgT8treatment
SA054688AgT15treatment
SA054689AgT2treatment
SA054690AgT9treatment
SA054691AgT5treatment
SA054692AgT3treatment
SA054693AgT4treatment
SA054694AgT6treatment
SA054695AgT12treatment
SA054696AgT11treatment
Showing results 1 to 51 of 51

Collection:

Collection ID:CO000953
Collection Summary:Briefly, diatoms Thalassiosira pseudonana strain CCMP 1335 and Asterionellopsis glacialis strain CCMP 137 were grown in silicate-amended L1 media in artificial seawater (Instant Ocean, 35 ppt). Karenia brevis strain CCMP 2228 was cultured in similar conditions above with L1 media-amended artificial seawater. All cultures were maintained at 21 ˚C with a 12:12 light/dark cycle and an irradiance of 100-145 µmol/m2s in a Percival incubator (Biospherical Instrument QSL2100).
Sample Type:Cell extract

Treatment:

Treatment ID:TR000973
Treatment Summary:To expose diatoms to competition with allelopathic K. brevis, K. brevis was co-cultured with each of the two diatom species (n=14 per species). K. brevis was grown inside a permeable dialysis membrane to allow for exchange of exuded allelopathic compounds without direct interaction of K. brevis and diatom cells, which were grown in flasks in which the dialysis tubes were placed. Control cultures consisted of dialysis membranes (molecular weight cutoff, 50 kDa) filled with L1 media diluted to conditions similar to that of exponential growth phase K. brevis (n = 15 per diatom species) in place of diatom species. This co-culture experiment was halted once competitor cultures reached exponential growth stage, which was 6 d for T. pseudonana and 8 d for A. glacialis, after which diatom cells were filtered onto GF/C filters (Whatman #1922-110, muffled at 450 ˚C for 3 h) and dipped into liquid nitrogen to quench intracellular metabolism.

Sample Preparation:

Sampleprep ID:SP000966
Sampleprep Summary:To separate polar and lipid intracellular metabolites, dried extracts were dissolved in a biphasic mixture of 9:10:15 water/methanol/chloroform. The more lipophilic layer was removed and washed twice with 9:10 water/methanol. Lipid extracts were reconstituted in 200 μL 2-propanol. Quantitative metabolomics data were acquired using a Waters Xevo G2 QTOF mass spectrometer.

Combined analysis:

Analysis ID AN001510
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity H-Class
Column Waters Acquity BEH C18 (50 x 2.1mm,1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Waters Synapt G2
Ion Mode NEGATIVE
Units Normalized Abundance

Chromatography:

Chromatography ID:CH001064
Instrument Name:Waters Acquity H-Class
Column Name:Waters Acquity BEH C18 (50 x 2.1mm,1.7um)
Column Temperature:60
Flow Gradient:0-1 min, 70% B; 1-3 min, 75% B; 3-6 min, 80% B; 6-10 min, 90% B; 10-14 min, 100% B.
Flow Rate:.3 mL/min
Solvent A:60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate
Solvent B:10% acetonitrile/90% isopropanol; 0.1% formic acid;; 10 mM ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS001393
Analysis ID:AN001510
Instrument Name:Waters Synapt G2
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Voltage:2.0
Source Temperature:90°C
Desolvation Gas Flow:600 L/h
Desolvation Temperature:250 °C
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