Summary of Study ST001107

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000740. The data can be accessed directly via it's Project DOI: 10.21228/M81D57 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001107
Study TitleNMR Metabolomics of Newborn Heart Tissue Exposed to Excess Maternal Cortisol in Late Gestation (part -II)
Study TypeComparison
Study SummaryCardiac tissue from newborn hearts from animals exposed to excess maternal cortisol in late gestation and untreated was compared via NMR metabolomic analysis
Institute
University of Florida
DepartmentBiochemsitry & Molecular Biology
Last NameWalejko
First NameJacquelyn
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Emailjwalejko@uga.edu
PhoneNA
Submit Date2018-12-04
Num Groups2
Total Subjects36
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2019-03-06
Release Version1
Jacquelyn Walejko Jacquelyn Walejko
https://dx.doi.org/10.21228/M81D57
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000740
Project DOI:doi: 10.21228/M81D57
Project Title:Chronic Maternal Cortisol Excess During Late Gestation Leads to Metabolic Alterations in the Newborn Heart
Project Summary:Our laboratory has previously shown in an ovine model of pregnancy that abnormal elevations in maternal cortisol during late gestation lead to increased fetal cardiac arrhythmias and mortality during peripartum. Furthermore, transcriptomic analysis of the fetal heart suggested alterations in TCA cycle intermediates and lipid metabolites in animals exposed to excess cortisol in utero. Therefore, we utilized a sheep model of pregnancy to determine how chronic increases in maternal cortisol alter maternal and fetal serum prior to birth and neonatal cardiac metabolites and lipids at term. Ewes were either infused with 1 mg/kg/day of cortisol starting at gestational day 115 (n=9), or untreated (n=6). Serum was collected from the mother and fetus (125 d-birth), and hearts were collected following birth. Proton nuclear magnetic resonance (1H-NMR) spectroscopy was conducted to measure metabolic profiles of newborn heart specimens as well as fetal and maternal serum specimens. Mass spectrometry was conducted to measure lipid profiles of newborn heart specimens. We observed alterations in amino acid and TCA cycle metabolism as well as lipid and glycerophospholipid metabolism in newborn hearts after excess maternal cortisol in late gestation. In addition, we observed alterations in amino acid and TCA cycle metabolites in fetal but not in maternal serum during late gestation. These results suggest that fetal exposure to excess maternal cortisol alters placental and fetal metabolism prior to birth and limits normal cardiac metabolic maturation, which may contribute to increased risk of peripartum cardiac arrhythmias observed in these animals, or later life cardiomyopathies.
Institute:University of Florida
Department:Pharmacodynamics
Last Name:Keller-Wood
First Name:Maureen
Address:1345 SW Archer Rd, PO 100487, Gainesville, FL, 32610
Email:kellerwd@cop.ufl.edu
Phone:NA

Subject:

Subject ID:SU001152
Subject Type:Mammal
Subject Species:Ovis aries
Taxonomy ID:9940
Age Or Age Range:Within 6 hours of birth

Factors:

Subject type: Mammal; Subject species: Ovis aries (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Heart Area
SA0752531610-16_LVControl Left_ventricle
SA075254753_LVControl Left_ventricle
SA075255767_LVControl Left_ventricle
SA075256734_LVControl Left_ventricle
SA0752571577_LVControl Left_ventricle
SA075258770_LVControl Left_ventricle
SA075259734_RVControl Right_ventricle
SA075260767_RVControl Right_ventricle
SA075261753_RVControl Right_ventricle
SA0752621610-16_RVControl Right_ventricle
SA0752631577_RVControl Right_ventricle
SA075264770_RVControl Right_ventricle
SA0752651577_SControl Septum
SA075266734_SControl Septum
SA075267753_SControl Septum
SA075268770_SControl Septum
SA075269767_SControl Septum
SA0752701610-16_SControl Septum
SA075271729_LVCortisol Left_ventricle
SA075272706_LVCortisol Left_ventricle
SA0752731576_LVCortisol Left_ventricle
SA0752743843_LVCortisol Left_ventricle
SA075275802_LVCortisol Left_ventricle
SA075276717_LVCortisol Left_ventricle
SA075277706_RVCortisol Right_ventricle
SA075278717_RVCortisol Right_ventricle
SA0752793843_RVCortisol Right_ventricle
SA0752801576_RVCortisol Right_ventricle
SA075281802_RVCortisol Right_ventricle
SA075282729_RVCortisol Right_ventricle
SA075283706_SCortisol Septum
SA0752841576_SCortisol Septum
SA075285729_SCortisol Septum
SA0752863843_SCortisol Septum
SA075287802_SCortisol Septum
SA075288717_SCortisol Septum
Showing results 1 to 36 of 36

Collection:

Collection ID:CO001146
Collection Summary:Immediately following birth, the lambs were euthanized with an overdose of Euthasol (pentobarbital sodium and phenytoin sodium; Virbac AH Inc), and heart tissue was collected from the right ventricle (RV), left ventricle (LV), and intraventricular septum (IVS) and immediately frozen in liquid nitrogen. Tissue samples were stored at -80 °C until data collection.
Sample Type:Cardiac tissue
Collection Method:Heart tissue was collected under sterile conditions and immediately frozen in liquid nitrogen
Collection Location:University of Florida
Storage Conditions:-80℃
Collection Vials:Cryovials
Storage Vials:Cryovials

Treatment:

Treatment ID:TR001166
Treatment Summary:The treatment protocol for this study was previous published in Antolic, A., et al. (2018). Chronic maternal hypercortisolemia in late gestation alters fetal cardiac function at birth. Am J Physiol Regul Integr Comp Physiol 314(3): R342-R352.
Treatment Compound:Hydrocortisone sodium succinate in sodium phosphate (Solu-Cortef; Pfizer, New York, NY, USA)
Treatment Dose:1 mg/kg/day

Sample Preparation:

Sampleprep ID:SP001159
Sampleprep Summary:Heart tissue (20-50 mg) was cut and weighed on dry ice before being placed on a sterile agar plate. Thirty μL of deuterium oxide (D2O) was added to each tissue sample before being placed in a 4 mm HR-MAS rotor (Bruker Biospin, Billerica, MA, USA) and leftover D2O (from original 30 μL) was added to the rotor with a pipette after placement of tissue. The samples were kept on dry ice until data acquisition.
Sampleprep Protocol Comments:Organ: Heart

Analysis:

Analysis ID:AN001801
Laboratory Name:Advanced Magnetic Resonance Imaging and Spectroscopy Facility-University of Florida
Analysis Type:NMR
Analysis Protocol File:Hearts_CORT_raw_data_noesy.zip
Acquisition Parameters File:Hearts_CORT_raw_data_noesy.zip
Software Version:Topspin 3.6
Operator Name:Jacquelyn Walejko
Processing Parameters File:Hearts_CORT_raw_data_noesy.zip
Num Factors:6
Num Metabolites:27
Units:Area under the curve

NMR:

NMR ID:NM000137
Analysis ID:AN001801
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Field Frequency Lock:H2O+D2O
Spectrometer Frequency:600 MHz
NMR Probe:4mm HR-MAS
NMR Solvent:D2O
NMR Tube Size:4mm HRMAS rotor
Shimming Method:Topshim
Pulse Sequence:noesypr1d
Water Suppression:Presaturation
Power Level:18 W
Receiver Gain:40.3
Offset Frequency:2819.74
Presaturation Power Level:3.98E-05
Temperature:4C
Number Of Scans:128
Dummy Scans:8
Acquisition Time:1.3598 sec
Relaxation Delay:2 sec
Spectral Width:10.0155 ppm
Num Data Points Acquired:16344
Real Data Points:32768
Line Broadening:2
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