Summary of Study ST001109

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000740. The data can be accessed directly via it's Project DOI: 10.21228/M81D57 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001109
Study TitleNMR Metabolomics of Newborn Heart Tissue Exposed to Excess Maternal Cortisol in Late Gestation - Maternal (part -IV)
Study TypeComparison
Study SummarySerum from mothers and fetuses exposed to excess maternal cortisol in late gestation and untreated was compared via NMR metabolomic analysis
Institute
University of Florida
DepartmentBiochemsitry & Molecular Biology
Last NameWalejko
First NameJacquelyn
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Emailjwalejko@uga.edu
PhoneNA
Submit Date2018-12-04
Num Groups2
Total Subjects36
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2019-03-06
Release Version1
Jacquelyn Walejko Jacquelyn Walejko
https://dx.doi.org/10.21228/M81D57
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000740
Project DOI:doi: 10.21228/M81D57
Project Title:Chronic Maternal Cortisol Excess During Late Gestation Leads to Metabolic Alterations in the Newborn Heart
Project Summary:Our laboratory has previously shown in an ovine model of pregnancy that abnormal elevations in maternal cortisol during late gestation lead to increased fetal cardiac arrhythmias and mortality during peripartum. Furthermore, transcriptomic analysis of the fetal heart suggested alterations in TCA cycle intermediates and lipid metabolites in animals exposed to excess cortisol in utero. Therefore, we utilized a sheep model of pregnancy to determine how chronic increases in maternal cortisol alter maternal and fetal serum prior to birth and neonatal cardiac metabolites and lipids at term. Ewes were either infused with 1 mg/kg/day of cortisol starting at gestational day 115 (n=9), or untreated (n=6). Serum was collected from the mother and fetus (125 d-birth), and hearts were collected following birth. Proton nuclear magnetic resonance (1H-NMR) spectroscopy was conducted to measure metabolic profiles of newborn heart specimens as well as fetal and maternal serum specimens. Mass spectrometry was conducted to measure lipid profiles of newborn heart specimens. We observed alterations in amino acid and TCA cycle metabolism as well as lipid and glycerophospholipid metabolism in newborn hearts after excess maternal cortisol in late gestation. In addition, we observed alterations in amino acid and TCA cycle metabolites in fetal but not in maternal serum during late gestation. These results suggest that fetal exposure to excess maternal cortisol alters placental and fetal metabolism prior to birth and limits normal cardiac metabolic maturation, which may contribute to increased risk of peripartum cardiac arrhythmias observed in these animals, or later life cardiomyopathies.
Institute:University of Florida
Department:Pharmacodynamics
Last Name:Keller-Wood
First Name:Maureen
Address:1345 SW Archer Rd, PO 100487, Gainesville, FL, 32610
Email:kellerwd@cop.ufl.edu
Phone:NA

Subject:

Subject ID:SU001154
Subject Type:Mammal
Subject Species:Ovis aries
Taxonomy ID:9940
Age Or Age Range:Gestational days 125-birth

Factors:

Subject type: Mammal; Subject species: Ovis aries (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Source
SA075467F_1616-10_140Control Fetal
SA075468F_753_135Control Fetal
SA075469F_728_144Control Fetal
SA075470F_734_143Control Fetal
SA075471F_728_140Control Fetal
SA075472F_759_138Control Fetal
SA075473F_728_142Control Fetal
SA075474F_767_135Control Fetal
SA075475F_753_130Control Fetal
SA075476F_1618_147Control Fetal
SA075477F_759_142Control Fetal
SA075478F_759_145Control Fetal
SA075479F_734_140Control Fetal
SA075480F_759_130Control Fetal
SA075481F_734_138Control Fetal
SA075482F_1618_144Control Fetal
SA075483F_1618_142Control Fetal
SA075484F_734_142Control Fetal
SA075485F_1618_148Control Fetal
SA075486F_1577_125Control Fetal
SA075487F_753_143Control Fetal
SA075488F_1618_149Control Fetal
SA075489F_1618_145Control Fetal
SA075490F_1618_125Control Fetal
SA075491F_767_130Control Fetal
SA075492F_753_138Control Fetal
SA075493F_767_125Control Fetal
SA075494F_1618_150Control Fetal
SA075495F_1616-10_125Control Fetal
SA075496F_759_144Control Fetal
SA075497F_734_130Control Fetal
SA075498F_734_125Control Fetal
SA075499F_734_144Control Fetal
SA075500F_753_140Control Fetal
SA075501F_1618_130Control Fetal
SA075502F_753_125Control Fetal
SA075503F_759_147Control Fetal
SA075504F_1616-10_137Control Fetal
SA075505F_1618_146Control Fetal
SA075506F_1577_140Control Fetal
SA075507F_1618_138Control Fetal
SA075508F_759_140Control Fetal
SA075509F_1577_138Control Fetal
SA075510F_770_130Control Fetal
SA075511F_1577_120Control Fetal
SA075512F_728_146Control Fetal
SA075513F_1577_130Control Fetal
SA075514F_759_125Control Fetal
SA075515F_770_135Control Fetal
SA075516F_759_146Control Fetal
SA075517F_1616-10_130Control Fetal
SA075518F_1618_143Control Fetal
SA075519F_1618_140Control Fetal
SA075520F_728_135Control Fetal
SA075521F_734_135Control Fetal
SA075522F_728_147Control Fetal
SA075523F_728_130Control Fetal
SA075524F_1618_141Control Fetal
SA075525F_728_138Control Fetal
SA075526F_770_140Control Fetal
SA075527F_1618_135Control Fetal
SA075528F_770_125Control Fetal
SA075529F_770_138Control Fetal
SA075530F_759_135Control Fetal
SA075531F_1577_135Control Fetal
SA075532M_1577_120Control Maternal
SA075533M_1616-10_140Control Maternal
SA075534M_753_125Control Maternal
SA075535M_1618_140Control Maternal
SA075536M_767_138Control Maternal
SA075537M_1577_140Control Maternal
SA075538M_1618_141Control Maternal
SA075539M_1618_135Control Maternal
SA075540M_1618_146Control Maternal
SA075541M_1618_130Control Maternal
SA075542M_1577_130Control Maternal
SA075543M_1577_135Control Maternal
SA075544M_1577_125Control Maternal
SA075545M_1618_144Control Maternal
SA075546M_1616-10_137Control Maternal
SA075547M_753_130Control Maternal
SA075548M_753_138Control Maternal
SA075549M_1577_138Control Maternal
SA075550M_767_130Control Maternal
SA075551M_1616-10_130Control Maternal
SA075552M_1618_138Control Maternal
SA075553M_767_135Control Maternal
SA075554M_759_144Control Maternal
SA075555M_734_144Control Maternal
SA075556M_1618_145Control Maternal
SA075557M_734_135Control Maternal
SA075558M_759_147Control Maternal
SA075559M_728_135Control Maternal
SA075560M_759_145Control Maternal
SA075561M_1618_142Control Maternal
SA075562M_1618_148Control Maternal
SA075563M_728_130Control Maternal
SA075564M_770_138Control Maternal
SA075565M_728_147Control Maternal
SA075566M_759_135Control Maternal
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Collection:

Collection ID:CO001148
Collection Summary:Blood samples were collected from ewe and fetus on gestational days 125, 130, 135, 138, 140, and every subsequent day until birth (control: 144±1; CORT: 142±2 days gestational age);
Sample Type:Blood (serum)
Collection Method:samples for metabolomics analysis were collected in glass tubes on ice with no anticoagulant and spun for 20 min at 4 °C; the resulting serum was stored at -20 °C until metabolomic analysis.
Collection Location:University of Florida
Storage Conditions:-20℃
Collection Vials:Eppendorf tubes
Storage Vials:Eppendorf tubes

Treatment:

Treatment ID:TR001168
Treatment Summary:The treatment protocol for this study was previous published in Antolic, A., et al. (2018). Chronic maternal hypercortisolemia in late gestation alters fetal cardiac function at birth. Am J Physiol Regul Integr Comp Physiol 314(3): R342-R352.
Treatment Compound:Hydrocortisone sodium succinate in sodium phosphate (Solu-Cortef; Pfizer, New York, NY, USA)
Treatment Dose:1 mg/kg/day

Sample Preparation:

Sampleprep ID:SP001161
Sampleprep Summary:Saline buffer was created by weighing 9 g of NaCl into a 1 L volumetric flask. Next, 100 mL of D2O was added and filled up to 1 L with water and shaken until dissolved. Samples were thawed on ice and centrifuged at 3,000 g for 20 min at 4 °C. Two hundred mL of serum was mixed with 400 mL of Saline buffer and centrifuged for 10 min at 3000 g at 4 °C. For each sample, 500 mL of the resulting supernatant was transferred into a 5 mm SampleJet NMR tube (Bruker Biospin, Billerica, MA, USA).

Analysis:

Analysis ID:AN001803
Laboratory Name:Advanced Magnetic Resonance Imaging and Spectroscopy Facility-University of Florida
Analysis Type:NMR
Analysis Protocol File:Serum_CORT_raw_data_cpmg.zip
Acquisition Parameters File:Serum_CORT_raw_data_cpmg.zip
Software Version:Topspin 3.6
Operator Name:Jacquelyn Walejko
Processing Parameters File:Serum_CORT_raw_data_cpmg.zip
Num Factors:4
Num Metabolites:29
Units:Area under curve

NMR:

NMR ID:NM000139
Analysis ID:AN001803
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Field Frequency Lock:H2O+D2O
Spectrometer Frequency:600 MHz
NMR Probe:5mm Cryoprobe
NMR Solvent:D2O
NMR Tube Size:5mm
Shimming Method:Topshim
Pulse Sequence:cpmgpr1d
Water Suppression:Presaturation
Power Level:7.37 W
Receiver Gain:64
Offset Frequency:2824.2
Presaturation Power Level:6.41E-05
Temperature:4C
Number Of Scans:128
Dummy Scans:16
Acquisition Time:1.8173
Relaxation Delay:4 sec
Spectral Width:15.0182 ppm
Num Data Points Acquired:32764
Real Data Points:65536
Line Broadening:1
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