Summary of Study ST001250
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000837. The data can be accessed directly via it's Project DOI: 10.21228/M8H118 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001250 |
| Study Title | Luminal metabolome profiles of mice developed dextran sulfate sodium (DSS) colitis |
| Study Summary | Luminal (cecal) metabolome of DSS-colitis mice and control mice were compared. |
| Institute | University of Michigan |
| Department | Internal Medicine |
| Last Name | Kamada |
| First Name | Nobuhiko |
| Address | 1150 W Medical center Dr, Ann Arbor, MI 48109, USA |
| nkamada@umich.edu | |
| Phone | +1-734-763-2142 |
| Submit Date | 2019-09-12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-10-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000837 |
| Project DOI: | doi: 10.21228/M8H118 |
| Project Title: | Luminal metabolome profiles of mice developed dextran sulfate sodium (DSS) colitis |
| Project Summary: | Intestinal inflammation alters the composition of the gut microbiota thereby changing luminal metabolite profile. To examine the changes in the luminal metabolites during intestinal inflammation, specific pathogen-free mice were given dextran sulfate sodium (DSS) to induce colitis. Cecal contents from DSS-induced colitis mice and control mice were harvested and metabolite profiles of them were analyzed. |
| Institute: | University of Michigan |
| Last Name: | Kamada |
| First Name: | Nobuhiko |
| Address: | 1150 W Medical center Dr, Ann Arbor, MI 48109, USA |
| Email: | nkamada@umich.edu |
| Phone: | +1-734-763-2142 |
Subject:
| Subject ID: | SU001318 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA091006 | 101901 | Control |
| SA091007 | 101905 | Control |
| SA091008 | 101904 | Control |
| SA091009 | 101902 | Control |
| SA091010 | 101903 | Control |
| SA091011 | 101910 | DSS |
| SA091012 | 101909 | DSS |
| SA091013 | 101906 | DSS |
| SA091014 | 101907 | DSS |
| SA091015 | 101908 | DSS |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO001312 |
| Collection Summary: | Cecal contents of DSS-treated and control mice were harvested. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR001333 |
| Treatment Summary: | SPF C57BL/6 mice were originally purchased from Jackson Laboratories. Mice were treated with 1.5% DSS for 5 days. |
Sample Preparation:
| Sampleprep ID: | SP001326 |
| Sampleprep Summary: | Fecal samples were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using Shake Master (Biomedical Science). Fecal metabolites were extracted using the methanol:chloroform:water extraction protocol. |
Combined analysis:
| Analysis ID | AN002075 | AN002076 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent CE | Agilent CE |
| Column | Fused-silica,50um(cation) | COSMO(+),50um(anion) |
| MS Type | ESI | ESI |
| MS instrument type | TOF | TOF |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | nmol/g | nmol/g |
Chromatography:
| Chromatography ID: | CH001512 |
| Instrument Name: | Agilent CE |
| Column Name: | Fused-silica,50um(cation) |
| Internal Standard: | 20 μM of methionine sulfone |
| Chromatography Type: | CE |
| Chromatography ID: | CH001513 |
| Instrument Name: | Agilent CE |
| Column Name: | COSMO(+),50um(anion) |
| Internal Standard: | 20 μM of D-camphor-10-sulfonic acid (CSA) |
| Chromatography Type: | CE |
MS:
| MS ID: | MS001926 |
| Analysis ID: | AN002075 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | POSITIVE |
| MS ID: | MS001927 |
| Analysis ID: | AN002076 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | NEGATIVE |
