Summary of Study ST001301
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000882. The data can be accessed directly via it's Project DOI: 10.21228/M8PD88 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001301 |
| Study Title | Luminal metabolome profiles of human microbiota-associated (HMA) mice treated with anti-IL-22 antibody or control antibody |
| Study Summary | Luminal (fecal) metabolome of anti-IL-22 antibody-treated HMA mice and control antibody-treated HMA mice were compared. |
| Institute | University of Michigan |
| Department | Internal Medicine |
| Last Name | Kamada |
| First Name | Nobuhiko |
| Address | 1150 W Medical center Dr, Ann Arbor, MI 48109, USA |
| nkamada@umich.edu | |
| Phone | +1-734-763-2142 |
| Submit Date | 2019-12-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-01-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000882 |
| Project DOI: | doi: 10.21228/M8PD88 |
| Project Title: | Luminal metabolome profiles of human microbiota-associated (HMA) mice treated with anti-IL-22 antibody or control antibody |
| Project Summary: | We found that healthy (HC)-HMA mice, GF mice were colonized with gut microbiota derived from human HC, were protected from Clostridioides difficile (C. difficile) infection. However, the colonization resistance of HC-HMA mice was abolished by treatment of anti-IL-22 antibody, unlike control antibody-treated HC-HMA mice. Additionally, the gut microbial composition of anti-IL22 antibody-treated HC-HMA mice, C. difficile-susceptible mice, were differ from that of control antibody, thereby it appears that luminal metabolome is changed between 2 groups. To examine the change in the luminal metabolites, fecal samples were collected from anti-IL-22 /control -antibody treated HC-HMA mice, and metabolite profiles of them were analyzed. |
| Institute: | University of Michigan |
| Department: | Internal Medicine |
| Last Name: | Kamada |
| First Name: | Nobuhiko |
| Address: | 1150 W Medical center Dr, Ann Arbor, MI 48109, USA |
| Email: | nkamada@umich.edu |
| Phone: | +1-734-763-2142 |
Subject:
| Subject ID: | SU001375 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA094259 | HC-HMA-Rag1KO+aIL-22 Ab #13 | Anti-IL-22 antibody |
| SA094260 | HC-HMA-Rag1KO+aIL-22 Ab #14 | Anti-IL-22 antibody |
| SA094261 | HC-HMA-Rag1KO+aIL-22 Ab #12 | Anti-IL-22 antibody |
| SA094262 | HC-HMA-Rag1KO+aIL-22 Ab #11 | Anti-IL-22 antibody |
| SA094263 | Ab6 | Anti-IL-22 antibody |
| SA094264 | HC-HMA-Rag1KO+aIL-22 Ab #15 | Anti-IL-22 antibody |
| SA094265 | HC-Rag1KO+aIL-22l Ab #11 | Anti-IL-22 antibody |
| SA094266 | HC-Rag1KO+aIL-22l Ab #15 | Anti-IL-22 antibody |
| SA094267 | HC-Rag1KO+aIL-22l Ab #14 | Anti-IL-22 antibody |
| SA094268 | HC-Rag1KO+aIL-22l Ab #13 | Anti-IL-22 antibody |
| SA094269 | HC-Rag1KO+aIL-22l Ab #12 | Anti-IL-22 antibody |
| SA094270 | Ab5 | Anti-IL-22 antibody |
| SA094271 | Ab4 | Anti-IL-22 antibody |
| SA094272 | HC-HMA-Rag1KO+Ctrl Ab #7 | Control antibody |
| SA094273 | HC-HMA-Rag1KO+Ctrl Ab #8 | Control antibody |
| SA094274 | HC-HMA-Rag1KO+Ctrl Ab #6 | Control antibody |
| SA094275 | Ab3 | Control antibody |
| SA094276 | Ab2 | Control antibody |
| SA094277 | HC-HMA-Rag1KO+Ctrl Ab #9 | Control antibody |
| SA094278 | HC-HMA-Rag1KO+Ctrl Ab #10 | Control antibody |
| SA094279 | HC-Rag1KO+Ctrl Ab #9 | Control antibody |
| SA094280 | HC-Rag1KO+Ctrl Ab #10 | Control antibody |
| SA094281 | HC-Rag1KO+Ctrl Ab #8 | Control antibody |
| SA094282 | HC-Rag1KO+Ctrl Ab #7 | Control antibody |
| SA094283 | HC-Rag1KO+Ctrl Ab #6 | Control antibody |
| SA094284 | Ab1 | Control antibody |
| Showing results 1 to 26 of 26 |
Collection:
| Collection ID: | CO001370 |
| Collection Summary: | Feces of anti-IL-22 antibody/control antibody-treated HC-HMA mice were harvested. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR001390 |
| Treatment Summary: | GF C57BL/6 mice were colonized with HC microbiotas for 2 weeks. After colonization of HC microbiota, the mice were treated with control or anti-IL-22 antibody twice (day -5 and day -3) before collecting fecal samples. |
Sample Preparation:
| Sampleprep ID: | SP001383 |
| Sampleprep Summary: | Fecal samples were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using Shake Master (Biomedical Science). Fecal metabolites were extracted using the methanol:chloroform:water extraction protocol. |
Chromatography:
| Chromatography ID: | CH001584 |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | Fused-silica,50um(cation) |
| Internal Standard: | 20 μM of methionine sulfone |
| Chromatography Type: | CE |
| Chromatography ID: | CH001585 |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | COSMO(+),50um(anion |
| Internal Standard: | 20 μM of D-camphor-10-sulfonic acid (CSA) |
| Chromatography Type: | CE |
Analysis:
| Analysis ID: | AN002166 |
| Analysis Type: | MS |
| Chromatography ID: | CH001584 |
| Num Factors: | 2 |
| Num Metabolites: | 120 |
| Units: | nmol/g |
| Analysis ID: | AN002167 |
| Analysis Type: | MS |
| Chromatography ID: | CH001585 |
| Num Factors: | 2 |
| Num Metabolites: | 62 |
| Units: | nmol/g |
