Summary of Study ST001396

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000952. The data can be accessed directly via it's Project DOI: 10.21228/M8N397 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001396
Study TitleQuantitative Lipids study on total murine liver tissue from mice at different age
Study TypeLiver tissue/Primary tissue
Study SummaryFollowing birth, the neonatal intestine is exposed to maternal and environmental bacteria that successively form a dense and highly dynamic intestinal microbiota. Whereas the effect of exogenous factors has been extensively investigated, endogenous, host-mediated mechanisms have remained largely unexplored. Concomitantly with microbial colonization, the liver undergoes functional transition from a hematopoietic organ to a central organ of metabolic regulation and immune surveillance. The aim of the present study was to analyze the influence of the developing hepatic function and liver metabolism on the early intestinal microbiota. Using metabolomic and microbial profiling in combination with multivariate analysis we characterized the colonization dynamics and liver metabolism in the murine gastrointestinal tract (n=6-10 per age group). We observed major age-dependent microbial and metabolic changes and identified bile acids as potent drivers of the early intestinal microbiota maturation. Consistently, oral administration of tauro-cholic acid or β-tauro-murocholic acid to newborn mice (n= 7-14 per group) accelerated postnatal microbiota maturation. Lipids in total liver tissue from healthy C57BL/6 mice at 1, 7, 14, 21, 28 and 56 day after birth was analyzed.
Institute
Helmholtz Centre for Environmental Research
DepartmentDepartment of Molecular Systems biology
LaboratoryFunctional Metabolomics
Last NameRolle-Kampczyk
First NameUlrike
AddressPermoserstrasse 15, 04318 Leipzig, Germany
Emailulrike.rolle-kampczyk@ufz.de
Phone0049 341 235 1537
Submit Date2020-06-03
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2020-06-10
Release Version1
Ulrike Rolle-Kampczyk Ulrike Rolle-Kampczyk
https://dx.doi.org/10.21228/M8N397
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000952
Project DOI:doi: 10.21228/M8N397
Project Title:Enteric microbiota and liver metabolomics during the postnatal period
Project Summary:Following birth, the neonatal intestine is exposed to maternal and environmental bacteria that successively form a dense and highly dynamic intestinal microbiota. Whereas the effect of exogenous factors has been extensively investigated, endogenous, host-mediated mechanisms have remained largely unexplored. Concomitantly with microbial colonization, the liver undergoes functional transition from a hematopoietic organ to a central organ of metabolic regulation and immune surveillance. The aim of the present study was to analyze the influence of the developing hepatic function and liver metabolism on the early intestinal microbiota. Using metabolomic and microbial profiling in combination with multivariate analysis we characterized the colonization dynamics and liver metabolism in the murine gastrointestinal tract (n=6-10 per age group). We observed major age-dependent microbial and metabolic changes and identified bile acids as potent drivers of the early intestinal microbiota maturation. Consistently, oral administration of tauro-cholic acid or β-tauro-murocholic acid to newborn mice (n= 7-14 per group) accelerated postnatal microbiota maturation.
Institute:Helmholtz Centre for Environmental Research - UFZ
Last Name:Haange
First Name:Sven
Address:Permoserstrasse 1, Leipzig, Saxony, 04318, Germany
Email:sven.haange@ufz.de
Phone:0049 341 2351099

Subject:

Subject ID:SU001470
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Litter Day after birth
SA113512L_K10D1_pos10 1
SA113513L_K10D14_pos10 14
SA113514L_K10D21_pos10 21
SA113515L_K10D28_pos10 28
SA113516L_K10D56_pos10 56
SA113517L_K10D7_pos10 7
SA113518L_K11D1_pos11 1
SA113519L_K11D14_pos11 14
SA113520L_K11D21_pos11 21
SA113521L_K11D28_pos11 28
SA113522L_K11D56_pos11 56
SA113523L_K11D7_pos11 7
SA113524L_K7D14_pos7 14
SA113525L_K7D21_pos7 21
SA113526L_K7D28_pos7 28
SA113527L_K7D56_pos7 56
SA113528L_K7D7_pos7 7
SA113529L_K8D14_pos8 14
SA113530L_K8D21_pos8 21
SA113531L_K8D28_pos8 28
SA113532L_K8D56_pos8 56
SA113533L_K8D7_pos8 7
SA113534L_K9D1_pos9 1
SA113535L_K9D14_pos9 14
SA113536L_K9D21_pos9 21
SA113537L_K9D28_pos9 28
SA113538L_K9D56_pos9 56
SA113539L_K9D7_pos9 7
Showing results 1 to 28 of 28

Collection:

Collection ID:CO001465
Collection Summary:Animals were sacrificed and the liver tissue was removed, snap frozen in liquid nitrogen and stored at -80°C until further analysis
Sample Type:Liver

Treatment:

Treatment ID:TR001485
Treatment Summary:All C57BL6J wildtype mice were bred locally and held under specific pathogen-free conditions at the Institute of Laboratory Animal Science at RWTH Aachen University Hospital. The day of birth was considered day 0, i.e. animals screened at day 1 were approximately 24 h old and verified to have ingested breast milk (abdominal milk spot). Mice were weaned at PND21.

Sample Preparation:

Sampleprep ID:SP001478
Sampleprep Summary:Solvents: Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS) Water MiliQ, Extracting agent - ACN / H2O (1:1) Equipment 4 steel balls size M Eppendorf Tubes 2mL Tissue slicer (Rettberg, Germany) Centrifuge (Sigma) Work steps Approximately 100mg of sample and 4 steel balls of size M into each tube. Per mg of sample 5µL of extracting agent was added. Shake the samples for 10 minutes at 30 Hz in the tissue slicer and centrifuge for 2 minutes at 14000 rpm. The supernatant was used for the targeted analytics. Kit reparation The analysis was performed using the validated MetIDQ p180 Kit and described in Siskos et al. [1]. Data processing was carried out with the provided quantitation method Kit (Biocrates Life Sciences AG, Innsbruck, Austria).

Combined analysis:

Analysis ID AN002335
Analysis type MS
Chromatography type Reversed phase
Chromatography system UPLC (Waters Acquity, Waters Corporation, Milford, USA)
Column Agilent Zorbax Eclipse XDB C18 (100 x 3.0mm,3.5um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap
Ion Mode POSITIVE
Units µM

Chromatography:

Chromatography ID:CH001711
Chromatography Comments:Agilent, Zorbax Eclipse XDB C18, 3.0 x 100 mm, 3.5 µM, Agilent Waldbronn, Germany
Instrument Name:UPLC (Waters Acquity, Waters Corporation, Milford, USA)
Column Name:Agilent Zorbax Eclipse XDB C18 (100 x 3.0mm,3.5um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002177
Analysis ID:AN002335
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Analyst version 1.6 Software from SCIEX. For Validation METIDQ Software version Boron from Biocrates
Ion Mode:POSITIVE
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