Summary of Study ST001397
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000952. The data can be accessed directly via it's Project DOI: 10.21228/M8N397 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001397 |
| Study Title | Quantitative Hexose study on total murine liver tissue from mice at different age |
| Study Type | Liver tissue/Primary tissue |
| Study Summary | Following birth, the neonatal intestine is exposed to maternal and environmental bacteria that successively form a dense and highly dynamic intestinal microbiota. Whereas the effect of exogenous factors has been extensively investigated, endogenous, host-mediated mechanisms have remained largely unexplored. Concomitantly with microbial colonization, the liver undergoes functional transition from a hematopoietic organ to a central organ of metabolic regulation and immune surveillance. The aim of the present study was to analyze the influence of the developing hepatic function and liver metabolism on the early intestinal microbiota. Using metabolomic and microbial profiling in combination with multivariate analysis we characterized the colonization dynamics and liver metabolism in the murine gastrointestinal tract (n=6-10 per age group). We observed major age-dependent microbial and metabolic changes and identified bile acids as potent drivers of the early intestinal microbiota maturation. Consistently, oral administration of tauro-cholic acid or β-tauro-murocholic acid to newborn mice (n= 7-14 per group) accelerated postnatal microbiota maturation.Summed hexoses in total liver tissue from healthy C57BL/6 mice at 1, 7, 14, 21, 28 and 56 day after birth was analyzed. |
| Institute | Helmholtz Centre for Environmental Research |
| Department | Department of Molecular Systems biology |
| Laboratory | Functional Metabolomics |
| Last Name | Rolle-Kampczyk |
| First Name | Ulrike |
| Address | Permoserstrasse 15, 04318 Leipzig, Germany |
| ulrike.rolle-kampczyk@ufz.de | |
| Phone | 0049 341 235 1537 |
| Submit Date | 2020-06-03 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-06-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000952 |
| Project DOI: | doi: 10.21228/M8N397 |
| Project Title: | Enteric microbiota and liver metabolomics during the postnatal period |
| Project Summary: | Following birth, the neonatal intestine is exposed to maternal and environmental bacteria that successively form a dense and highly dynamic intestinal microbiota. Whereas the effect of exogenous factors has been extensively investigated, endogenous, host-mediated mechanisms have remained largely unexplored. Concomitantly with microbial colonization, the liver undergoes functional transition from a hematopoietic organ to a central organ of metabolic regulation and immune surveillance. The aim of the present study was to analyze the influence of the developing hepatic function and liver metabolism on the early intestinal microbiota. Using metabolomic and microbial profiling in combination with multivariate analysis we characterized the colonization dynamics and liver metabolism in the murine gastrointestinal tract (n=6-10 per age group). We observed major age-dependent microbial and metabolic changes and identified bile acids as potent drivers of the early intestinal microbiota maturation. Consistently, oral administration of tauro-cholic acid or β-tauro-murocholic acid to newborn mice (n= 7-14 per group) accelerated postnatal microbiota maturation. |
| Institute: | Helmholtz Centre for Environmental Research - UFZ |
| Last Name: | Haange |
| First Name: | Sven |
| Address: | Permoserstrasse 1, Leipzig, Saxony, 04318, Germany |
| Email: | sven.haange@ufz.de |
| Phone: | 0049 341 2351099 |
Subject:
| Subject ID: | SU001471 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Litter | Day after birth |
|---|---|---|---|
| SA113540 | L_K10D1_neg | 10 | 1 |
| SA113541 | L_K10D14_neg | 10 | 14 |
| SA113542 | L_K10D21_neg | 10 | 21 |
| SA113543 | L_K10D28_neg | 10 | 28 |
| SA113544 | L_K10D56_neg | 10 | 56 |
| SA113545 | L_K10D7_neg | 10 | 7 |
| SA113546 | L_K11D1_neg | 11 | 1 |
| SA113547 | L_K11D14_neg | 11 | 14 |
| SA113548 | L_K11D21_neg | 11 | 21 |
| SA113549 | L_K11D28_neg | 11 | 28 |
| SA113550 | L_K11D56_neg | 11 | 56 |
| SA113551 | L_K11D7_neg | 11 | 7 |
| SA113552 | L_K7D14_neg | 7 | 14 |
| SA113553 | L_K7D21_neg | 7 | 21 |
| SA113554 | L_K7D28_neg | 7 | 28 |
| SA113555 | L_K7D56_neg | 7 | 56 |
| SA113556 | L_K7D7_neg | 7 | 7 |
| SA113557 | L_K8D14_neg | 8 | 14 |
| SA113558 | L_K8D21_neg | 8 | 21 |
| SA113559 | L_K8D28_neg | 8 | 28 |
| SA113560 | L_K8D56_neg | 8 | 56 |
| SA113561 | L_K8D7_neg | 8 | 7 |
| SA113562 | L_K9D1_neg | 9 | 1 |
| SA113563 | L_K9D14_neg | 9 | 14 |
| SA113564 | L_K9D21_neg | 9 | 21 |
| SA113565 | L_K9D28_neg | 9 | 28 |
| SA113566 | L_K9D56_neg | 9 | 56 |
| SA113567 | L_K9D7_neg | 9 | 7 |
| Showing results 1 to 28 of 28 |
Collection:
| Collection ID: | CO001466 |
| Collection Summary: | Animals were sacrificed and the liver tissue was removed, snap frozen in liquid nitrogen and stored at -80°C until further analysis |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR001486 |
| Treatment Summary: | All C57BL6J wildtype mice were bred locally and held under specific pathogen-free conditions at the Institute of Laboratory Animal Science at RWTH Aachen University Hospital. The day of birth was considered day 0, i.e. animals screened at day 1 were approximately 24 h old and verified to have ingested breast milk (abdominal milk spot). Mice were weaned at PND21. |
Sample Preparation:
| Sampleprep ID: | SP001479 |
| Sampleprep Summary: | Sample preparation for MetIDQ p180 Kit measurement Solvents: Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS) Water MiliQ, Extracting agent - ACN / H2O (1:1) Equipment 4 steel balls size M Eppendorf Tubes 2mL Tissue slicer (Rettberg, Germany) Centrifuge (Sigma) Work steps Approximately 100mg of sample and 4 steel balls of size M into each tube. Per mg of sample 5µL of extracting agent was added. Shake the samples for 10 minutes at 30 Hz in the tissue slicer and centrifuge for 2 minutes at 14000 rpm. The supernatant was used for the targeted analytics. Kit reparation The analysis was performed using the validated MetIDQ p180 Kit and described in Siskos et al. [1]. Data processing was carried out with the provided quantitation method Kit (Biocrates Life Sciences AG, Innsbruck, Austria). 1 Siskos AP, Jain P, Römisch-Margl W, Bennett M, Achaintre D, Asad Y, et al. Interlaboratory Reproducibility of a Targeted Metabolomics Platform for Analysis of Human Serum and Plasma. Anal Chem 2017;89:656-65. |
Combined analysis:
| Analysis ID | AN002336 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | UPLC (Waters Acquity, Waters Corporation, Milford, USA) |
| Column | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | NEGATIVE |
| Units | µM |
Chromatography:
| Chromatography ID: | CH001712 |
| Instrument Name: | UPLC (Waters Acquity, Waters Corporation, Milford, USA) |
| Column Name: | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002178 |
| Analysis ID: | AN002336 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Analyst version 1.6 Software from SCIEX. For Validation METIDQ Software version Boron from Biocrates |
| Ion Mode: | NEGATIVE |
