Summary of Study ST001508

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001017. The data can be accessed directly via it's Project DOI: 10.21228/M87M4S This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001508
Study TitleLiver metabolomics in old liver-specific SIRT6 overexpressing mice
Study SummaryOur previous data had suggested that gluconeogenesis capacity from the precursors lactate and glycerol declines in old C57BL/6 mice. This decline was rescued by whole body SIRT6 overexpression, but not in liver-specific SIRT6 overexpression. Here we preformed liver metabolomics in old liver-specific SIRT6 overexpression and control mice.
Institute
Bar Ilan University
DepartmentThe Mina & Everard Goodman Faculty of Life Sciences
LaboratoryLaboratory of Prof. Haim Cohen
Last NameCohen
First NameChaim
AddressBar Ilan University, Ramat Gan, Israel, 5290002
EmailHaim.Cohen@biu.ac.il
Phone+97235318383
Submit Date2020-10-07
Num Groups4
Total Subjects20
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-03-11
Release Version1
Chaim Cohen Chaim Cohen
https://dx.doi.org/10.21228/M87M4S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001017
Project DOI:doi: 10.21228/M87M4S
Project Title:Hepatic [U-13C]Lactate tracing and metabolomics in young and old WT and SIRT6 overexpressing mice
Project Type:MS analysis
Project Summary:Our previous data had suggested that gluconeogenesis capacity from the precursors lactate and glycerol declines in old C57BL/6 mice. This decline was rescued by whole body SIRT6 overexpression. Since the liver is the main gluconeogenic organ, we performed here liver metabolomics in young (6 months) versus old (20-24 months) WT and SIRT6-transgenic mice. We used liver tissues of 6h morning-fasted mice, either in naïve state or 15 minutes after intraperitoneal injection of [U-13C]Lactate.
Institute:Bar Ilan University
Department:The Mina & Everard Goodman Faculty of Life Sciences
Laboratory:Laboratory of Prof. Haim Cohen
Last Name:Cohen
First Name:Chaim
Address:Ramat Gan, Ramat Gan, Israel, 5290002, Israel
Email:Haim.Cohen@biu.ac.il
Phone:+97235318383
Funding Source:Israel Science Foundation (621/13 and 777/16), I-Core foundation (1775/12) and (41/11) and Laura and Isaac Perlmutter Foundation
Contributors:Asael Roichman, Ifat Abramovich, Eyal Gottlieb, Haim Yosef Cohen

Subject:

Subject ID:SU001582
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6JOlaHsd
Age Or Age Range:23-25 months
Gender:Male
Animal Animal Supplier:Envigo
Animal Housing:Animal rooms were maintained at 21°C-24°C and 35%-75% relative humidity
Animal Light Cycle:12/12-h (7am to 7pm) dark-light cycle
Animal Feed:Altromin 1324 (chow)
Animal Water:Ad lib

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA12679307_Liver_CREAlbumin Cre control
SA12679408_Liver_CREAlbumin Cre control
SA12679516_Liver_CREAlbumin Cre control
SA12679604_Liver_CREAlbumin Cre control
SA12679719_Liver_CREAlbumin Cre control
SA12679812_Liver_KISSFlox control
SA12679914_Liver_KISSFlox control
SA12680011_Liver_KISSFlox control
SA12680102_Liver_KISSFlox control
SA12680206_Liver_KISSFlox control
SA12680310_Liver_OELiver specific SIRT6 overexpression
SA12680403_Liver_OELiver specific SIRT6 overexpression
SA12680518_Liver_OELiver specific SIRT6 overexpression
SA12680601_Liver_OELiver specific SIRT6 overexpression
SA12680717_Liver_OELiver specific SIRT6 overexpression
SA12680820_Liver_WTWild-type
SA12680915_Liver_WTWild-type
SA12681009_Liver_WTWild-type
SA12681113_Liver_WTWild-type
SA12681205_Liver_WTWild-type
Showing results 1 to 20 of 20

Collection:

Collection ID:CO001577
Collection Summary:Mice were euthanized by cervical dislocation, left liver lobe was collected as fast as possible (<30 sec) and immediately flash-frozen in liquid nitrogen. Tissues were stored at -80C until metabolite extraction.
Sample Type:Liver
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001597
Treatment Summary:All mice were fasted for 6h (8AM-2PM). Tail blood was collected from all mice for less than 2 minutes, followed by mouse sacrifice.
Animal Fasting:6h morning fast
Animal Endp Euthanasia:Cervical dislocation

Sample Preparation:

Sampleprep ID:SP001590
Sampleprep Summary:Frozen liver pieces weighting ~30mg were transferred into soft tissue homogenizing CK 14 tubes containing 1.4 mm ceramic beads (Bertin corp.) prefilled with 1 ml of cold (-20°C) metabolites extraction solvent (Methanol: Acetonitrile:H2O, 50:30:20) and kept on ice. Samples were homogenized using precellys 24 tissue homogenizer (3X20sec at 6000 rpm, with a 30sec gap between each of the three cycles, Bertin Technologies) cooled to 2°C. Homogenized extracts were centrifuged in the Precellys tubes at 18,000g for 15 minutes at 4°C, supernatants were collected in microcentrifuge tubes and centrifuged again at 18,000g for 10 minutes at 4°C. The supernatants were transferred to glass HPLC vials and kept at -75 °C prior to LC-MS analysis.
Extract Storage:-80℃

Combined analysis:

Analysis ID AN002499
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Dionex Ultimate 3000
Column SeQuant ZIC-HILIC (150 x 2.1mm,5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units Peak Area

Chromatography:

Chromatography ID:CH001828
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:SeQuant ZIC-HILIC (150 x 2.1mm,5um)
Column Temperature:45
Flow Rate:0.2 ml/min
Solvent A:100% water; 20 mM ammonium carbonate adjusted to pH 9.2; 0.1% of 25% ammonium hydroxide
Solvent B:100% acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS002319
Analysis ID:AN002499
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:polarity switching mode to enable both positive and negative ions was used
Ion Mode:UNSPECIFIED
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