Summary of Study ST001523
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001005. The data can be accessed directly via it's Project DOI: 10.21228/M8SH6G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001523 |
| Study Title | The effect of fasting and sirtuin overexpression on serum metabolome. |
| Study Type | untargeted metabolomics |
| Study Summary | The levels of the sirtuins SIRT1 and SIRT6 are regulated by nutrient availability in several mammalian tissues. In addition, overexpressing SIRT1 in mice improves healthspan, while SIRT6 overexpression increases both healthspan and lifespan. However, little is known about the impact of these sirtuins at the in vivo metabolomics level. Thus, we performed metabolomics on serum taken from 15 months old male mice overexpressing SIRT1, SIRT6 and SIRT1+SIRT6 as well as WT controls. Sera were obtained from mice at three nutritional time points - at fed state, after 4h morning fast and after 16h fast. |
| Institute | Bar Ilan University |
| Department | The Mina & Everard Goodman Faculty of Life Sciences |
| Laboratory | Laboratory of Prof. Haim Cohen |
| Last Name | Cohen |
| First Name | Haim |
| Address | Bar Ilan University, Ramat Gan, Israel, 5290002 |
| Haim.Cohen@biu.ac.il | |
| Phone | +97235318383 |
| Submit Date | 2020-09-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2021-03-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001005 |
| Project DOI: | doi: 10.21228/M8SH6G |
| Project Title: | The effect of fasting and sirtuin overexpression on serum metabolome. |
| Project Type: | metabolomics |
| Project Summary: | The levels of the sirtuins SIRT1 and SIRT6 are regulated by nutrient availability in several mammalian tissues. In addition, overexpressing SIRT1 in mice improves healthspan, while SIRT6 overexpression increases both healthspan and lifespan. However, little is known about the impact of these sirtuins at the in vivo metabolomics level. Thus, we performed metabolomics on serum taken from 15 months old male mice overexpressing SIRT1, SIRT6 and SIRT1+SIRT6 as well as WT controls. Sera were obtained from mice at three nutritional time points - at fed state, after 4h morning fast and after 16h fast. |
| Institute: | Bar Ilan University |
| Department: | The Mina & Everard Goodman Faculty of Life Sciences |
| Laboratory: | Laboratory of Prof. Haim Cohen |
| Last Name: | Haim |
| First Name: | Cohen |
| Address: | Bar Ilan University, Ramat Gan, Israel, 5290002 |
| Email: | Haim.Cohen@biu.ac.il |
| Phone: | +97235318383 |
| Funding Source: | Intramural Research Program of the National Institute on Aging, NIH |
Subject:
| Subject ID: | SU001597 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treamtent group |
|---|---|---|
| SA128376 | (20) 1+6 16h_072 | Sirt1+6 OE - 16h fast |
| SA128377 | (39) 1+6 16h_075 | Sirt1+6 OE - 16h fast |
| SA128378 | (16) 1+6 16h_071 | Sirt1+6 OE - 16h fast |
| SA128379 | (28) 1+6 16h_073 | Sirt1+6 OE - 16h fast |
| SA128380 | (36) 1+6 16h_074 | Sirt1+6 OE - 16h fast |
| SA128381 | (16) 1+6 4h_066 | Sirt1+6 OE - 4h fast |
| SA128382 | (20) 1+6 4h_067 | Sirt1+6 OE - 4h fast |
| SA128383 | (28) 1+6 4h_068 | Sirt1+6 OE - 4h fast |
| SA128384 | (39) 1+6 4h_070 | Sirt1+6 OE - 4h fast |
| SA128385 | (36) 1+6 4h_069 | Sirt1+6 OE - 4h fast |
| SA128386 | (20) 1+6 fed_062 | Sirt1+6 OE - fed |
| SA128387 | (28) 1+6 fed_063 | Sirt1+6 OE - fed |
| SA128388 | (16) 1+6 fed_061 | Sirt1+6 OE - fed |
| SA128389 | (36) 1+6 fed_064 | Sirt1+6 OE - fed |
| SA128390 | (39) 1+6 fed_065 | Sirt1+6 OE - fed |
| SA128355 | (21) 1 16h_039 | Sirt1 OE - 16h fast |
| SA128356 | (8) 1 16h_037 | Sirt1 OE - 16h fast |
| SA128357 | (35) 1 16h_040 | Sirt1 OE - 16h fast |
| SA128358 | (52) 1 16h_041 | Sirt1 OE - 16h fast |
| SA128359 | (55) 1 16h_042 | Sirt1 OE - 16h fast |
| SA128360 | (6) 1 16h_036 | Sirt1 OE - 16h fast |
| SA128361 | (17) 1 16h_038 | Sirt1 OE - 16h fast |
| SA128362 | (35) 1 4h_033 | Sirt1 OE - 4h fast |
| SA128363 | (17) 1 4h_031 | Sirt1 OE - 4h fast |
| SA128364 | (8) 1 4h_030 | Sirt1 OE - 4h fast |
| SA128365 | (6) 1 4h_029 | Sirt1 OE - 4h fast |
| SA128366 | (52) 1 4h_034 | Sirt1 OE - 4h fast |
| SA128367 | (21) 1 4h_032 | Sirt1 OE - 4h fast |
| SA128368 | (55) 1 4h_035 | Sirt1 OE - 4h fast |
| SA128369 | (55) 1 fed_028 | Sirt1 OE - fed |
| SA128370 | (6) 1 fed_022 | Sirt1 OE - fed |
| SA128371 | (17) 1 fed_024 | Sirt1 OE - fed |
| SA128372 | (21) 1 fed_025 | Sirt1 OE - fed |
| SA128373 | (35) 1 fed_026 | Sirt1 OE - fed |
| SA128374 | (52) 1 fed_027 | Sirt1 OE - fed |
| SA128375 | (8) 1 fed_023 | Sirt1 OE - fed |
| SA128391 | (14) 6 16h_056 | Sirt6 OE - 16h fast |
| SA128392 | (13) 6 16h_055 | Sirt6 OE - 16h fast |
| SA128393 | (19) 6 16h_058 | Sirt6 OE - 16h fast |
| SA128394 | (15) 6 16h_057 | Sirt6 OE - 16h fast |
| SA128395 | (29) 6 16h_059 | Sirt6 OE - 16h fast |
| SA128396 | (38) 6 16h_060 | Sirt6 OE - 16h fast |
| SA128397 | (19) 6 4h_052 | Sirt6 OE - 4h fast |
| SA128398 | (15) 6 4h_051 | Sirt6 OE - 4h fast |
| SA128399 | (38) 6 4h_054 | Sirt6 OE - 4h fast |
| SA128400 | (14) 6 4h_050 | Sirt6 OE - 4h fast |
| SA128401 | (29) 6 4h_053 | Sirt6 OE - 4h fast |
| SA128402 | (13) 6 4h_049 | Sirt6 OE - 4h fast |
| SA128403 | (15) 6 fed_045 | Sirt6 OE - fed |
| SA128404 | (14) 6 fed_044 | Sirt6 OE - fed |
| SA128405 | (19) 6 fed_046 | Sirt6 OE - fed |
| SA128406 | (13) 6 fed_043 | Sirt6 OE - fed |
| SA128407 | (29) 6 fed_047 | Sirt6 OE - fed |
| SA128408 | (38) 6 fed_048 | Sirt6 OE - fed |
| SA128409 | (18) WT 16h_017 | WT - 16h fast |
| SA128410 | (7) WT 16h_015 | WT - 16h fast |
| SA128411 | (65) WT 16h_021 | WT - 16h fast |
| SA128412 | (9) WT 16h_016 | WT - 16h fast |
| SA128413 | (48) WT 16h_019 | WT - 16h fast |
| SA128414 | (27) WT 16h_018 | WT - 16h fast |
| SA128415 | (56) WT 16h_020 | WT - 16h fast |
| SA128416 | (65) WT 4h_014 | WT - 4h fast |
| SA128417 | (48) WT 4h_012 | WT - 4h fast |
| SA128418 | (18) WT 4h_010 | WT - 4h fast |
| SA128419 | (9) WT 4h_009 | WT - 4h fast |
| SA128420 | (27) WT 4h_011 | WT - 4h fast |
| SA128421 | (56) WT 4h_013 | WT - 4h fast |
| SA128422 | (7) WT 4h_008 | WT - 4h fast |
| SA128423 | (9) WT fed_002 | WT - Fed |
| SA128424 | (18) WT fed_003 | WT - Fed |
| SA128425 | (65) WT fed_007 | WT - Fed |
| SA128426 | (7) WT fed_001 | WT - Fed |
| SA128427 | (56) WT fed_006 | WT - Fed |
| SA128428 | (48) WT fed_005 | WT - Fed |
| SA128429 | (27) WT fed_004 | WT - Fed |
| Showing results 1 to 75 of 75 |
Collection:
| Collection ID: | CO001592 |
| Collection Summary: | 2 to 5 drops of blood were taken from the facial vein of each mouse using sterile 5mm goldenrod animal lancets. Blood was allowed to clot for 30-80 minutes at room temperature, then centrifuged 1,000 x g for 15 min at 4°C, snap frozen with liquid nitrogen and stored at -80°C. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR001612 |
| Treatment Summary: | 4 genotypes/3 time points, 5-7, Genotypes: WT, Sirt1-OE, Sirt6-OE, Sirt1+6-OE Time points: Fed - 4h fast- 16h fast |
Sample Preparation:
| Sampleprep ID: | SP001605 |
| Sampleprep Summary: | 2 to 5 drops of blood were taken from the facial vein of each mouse using sterile 5mm goldenrod animal lancets. Blood was allowed to clot for 30-80 minutes at room temperature, then centrifuged 1,000 x g for 15 min at 4°C, snap frozen with liquid nitrogen and stored at -80°C. |
Combined analysis:
| Analysis ID | AN002541 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus IV GC |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus IV TOF |
| Ion Mode | POSITIVE |
| Units | normalized peak height |
Chromatography:
| Chromatography ID: | CH001861 |
| Chromatography Summary: | primary metabolism by GCTOF |
| Instrument Name: | Leco Pegasus IV GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002359 |
| Analysis ID: | AN002541 |
| Instrument Name: | Leco Pegasus IV TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | A Leco Pegasus IV time of flight mass spectrometer is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The transfer line temperature between gas chromatograph and mass spectrometer is set to 280°C. Electron impact ionization at 70V is employed with an ion source temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass range of 85-500 Da. |
| Ion Mode: | POSITIVE |
