Summary of Study ST001750
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001122. The data can be accessed directly via it's Project DOI: 10.21228/M8P686 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001750 |
| Study Title | Gut microbiome influence on metabolic 1 disease in HIV and high-risk populations |
| Study Summary | Poor metabolic health, characterized by insulin resistance and dyslipidemia, is higher in people living with HIV (PLWH) and has been linked with inflammation, anti-retroviral therapy (ART) drugs, and ART-associated lipodystrophy (LD). Metabolic disease is associated with gut microbiome composition outside the context of HIV but has not been deeply explored in HIV infection nor in high-risk men who have sex with men (HR-MSM), who have a highly altered gut microbiome composition. Furthermore, the contribution of increased bacterial translocation and associated systemic inflammation that has been described in HIV-positive and HR-MSM individuals has not been explored. We used a multi-omic approach to explore relationships between gut microbes, immune phenotypes, diet, and metabolic health across ART-treated PLWH with and without LD; untreated PLWH; and HR-MSM. For PLWH on ART, we further explored associations with the plasma metabolome. |
| Institute | University of Colorado Anschutz Medical Campus |
| Last Name | Reisdorph |
| First Name | Nichole |
| Address | 12850 East Montview Blvd, Aurora, CO, 80045, USA |
| nichole.reisdorph@cuanschutz.edu | |
| Phone | 303-724-9234 |
| Submit Date | 2021-04-24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-05-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001122 |
| Project DOI: | doi: 10.21228/M8P686 |
| Project Title: | Gut microbiome influence on metabolic 1 disease in HIV and high-risk populations |
| Project Summary: | Poor metabolic health, characterized by insulin resistance and dyslipidemia, is higher in people living with HIV (PLWH) and has been linked with inflammation, anti-retroviral therapy (ART) drugs, and ART-associated lipodystrophy (LD). Metabolic disease is associated with gut microbiome composition outside the context of HIV but has not been deeply explored in HIV infection nor in high-risk men who have sex with men (HR-MSM), who have a highly altered gut microbiome composition. Furthermore, the contribution of increased bacterial translocation and associated systemic inflammation that has been described in HIV-positive and HR-MSM individuals has not been explored. We used a multi-omic approach to explore relationships between gut microbes, immune phenotypes, diet, and metabolic health across ART-treated PLWH with and without LD; untreated PLWH; and HR-MSM. For PLWH on ART, we further explored associations with the plasma metabolome. |
| Institute: | University of Colorado Anschutz Medical Campus |
| Last Name: | Reisdorph |
| First Name: | Nichole |
| Address: | 12850 East Montview Blvd, Aurora, CO, 80045, USA |
| Email: | nichole.reisdorph@cuanschutz.edu |
| Phone: | 303-724-9234 |
Subject:
| Subject ID: | SU001827 |
| Subject Type: | Mammal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample_Type | Lipodystrophy | HIV_status | Antiviral_Treatment |
|---|---|---|---|---|---|
| SA163875 | 9_mouse | Humanized mouse | - | Negative | No |
| SA163876 | 5_mouse | Humanized mouse | - | Negative | No |
| SA163877 | 4_mouse | Humanized mouse | - | Negative | No |
| SA163878 | 2_mouse | Humanized mouse | - | Negative | No |
| SA163879 | 6_mouse | Humanized mouse | - | Positive | No |
| SA163880 | 10_mouse | Humanized mouse | - | Positive | No |
| SA163881 | 3_mouse | Humanized mouse | - | Positive | Yes |
| SA163882 | 7_mouse | Humanized mouse | - | Positive | Yes |
| SA163831 | 57 | Human | NO | Positive | Yes |
| SA163832 | DM19 | Human | NO | Positive | Yes |
| SA163833 | 40 | Human | NO | Positive | Yes |
| SA163834 | DM003 | Human | NO | Positive | Yes |
| SA163835 | DM034 | Human | NO | Positive | Yes |
| SA163836 | DM002 | Human | NO | Positive | Yes |
| SA163837 | 9 | Human | NO | Positive | Yes |
| SA163838 | 128 | Human | NO | Positive | Yes |
| SA163839 | 112 | Human | NO | Positive | Yes |
| SA163840 | 36 | Human | NO | Positive | Yes |
| SA163841 | DM032 | Human | NO | Positive | Yes |
| SA163842 | 19 | Human | NO | Positive | Yes |
| SA163843 | 30 | Human | NO | Positive | Yes |
| SA163844 | 21 | Human | NO | Positive | Yes |
| SA163845 | DM052 | Human | NO | Positive | Yes |
| SA163846 | 23 | Human | NO | Positive | Yes |
| SA163847 | DM036 | Human | NO | Positive | Yes |
| SA163848 | 29 | Human | NO | Positive | Yes |
| SA163849 | 27 | Human | NO | Positive | Yes |
| SA163850 | 26 | Human | NO | Positive | Yes |
| SA163851 | 145 | Human | YES | Positive | Yes |
| SA163852 | 146 | Human | YES | Positive | Yes |
| SA163853 | 152 | Human | YES | Positive | Yes |
| SA163854 | 166 | Human | YES | Positive | Yes |
| SA163855 | 133 | Human | YES | Positive | Yes |
| SA163856 | 167 | Human | YES | Positive | Yes |
| SA163857 | 157 | Human | YES | Positive | Yes |
| SA163858 | 156 | Human | YES | Positive | Yes |
| SA163859 | 131 | Human | YES | Positive | Yes |
| SA163860 | 45 | Human | YES | Positive | Yes |
| SA163861 | 55 | Human | YES | Positive | Yes |
| SA163862 | 37 | Human | YES | Positive | Yes |
| SA163863 | 132 | Human | YES | Positive | Yes |
| SA163864 | 20 | Human | YES | Positive | Yes |
| SA163865 | 22 | Human | YES | Positive | Yes |
| SA163866 | 69 | Human | YES | Positive | Yes |
| SA163867 | 25 | Human | YES | Positive | Yes |
| SA163868 | 130 | Human | YES | Positive | Yes |
| SA163869 | 72 | Human | YES | Positive | Yes |
| SA163870 | 119 | Human | YES | Positive | Yes |
| SA163871 | 106 | Human | YES | Positive | Yes |
| SA163872 | 79 | Human | YES | Positive | Yes |
| SA163873 | 80 | Human | YES | Positive | Yes |
| SA163874 | 104 | Human | YES | Positive | Yes |
| SA163883 | 1_mouse | PBS Control | - | - | - |
| SA163884 | 8_mouse | PBS Control | - | - | - |
| Showing results 1 to 54 of 54 |
Collection:
| Collection ID: | CO001820 |
| Collection Summary: | Whole blood was collected in sodium heparin vacutainers and centrifuged at 1700rpm for 10 minutes for plasma collection. Plasma was aliquoted into 1mL microcentrifuge tubes and stored at -80. |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001840 |
| Treatment Summary: | n/a |
Sample Preparation:
| Sampleprep ID: | SP001833 |
| Sampleprep Summary: | See uploaded file |
| Sampleprep Protocol Filename: | Methods_-_Sample_Prep.pdf |
Combined analysis:
| Analysis ID | AN002852 | AN002853 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | Reversed phase |
| Chromatography system | Agilent 6545 QTOF | Agilent 6545 QTOF |
| Column | Agilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm) | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF |
| Ion Mode | POSITIVE | POSITIVE |
| Units | Abundance (Mass Spectral Counts) | Abundance (Mass Spectral Counts) |
Chromatography:
| Chromatography ID: | CH002111 |
| Instrument Name: | Agilent 6545 QTOF |
| Column Name: | Agilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH002112 |
| Instrument Name: | Agilent 6545 QTOF |
| Column Name: | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002645 |
| Analysis ID: | AN002852 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Methods_-_LCMS.pdf |
| Ion Mode: | POSITIVE |
| MS ID: | MS002646 |
| Analysis ID: | AN002853 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Methods_-_LCMS.pdf |
| Ion Mode: | POSITIVE |
