Summary of Study ST001882
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001186. The data can be accessed directly via it's Project DOI: 10.21228/M8DD61 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001882 |
Study Title | LC-MS for Predator Cues Target Signaling Pathways in Toxic Algal Metabolome Protocol |
Study Summary | Metabolomics investigation of the phytoplankton Alexandrium minutum with and without copepod cues in order to explore cell signaling involved in toxin induction. |
Institute | Georgia Institute of Technology |
Last Name | Brown |
First Name | Emily |
Address | 950 Atlantic Drive, Atlanta GA 30332 |
emily.brown@gatech.edu | |
Phone | 404-894-8424 |
Submit Date | 2021-07-15 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2021-07-27 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR001186 |
Project DOI: | doi: 10.21228/M8DD61 |
Project Title: | Predator Cues Target Signaling Pathways in Toxic Algal Metabolome |
Project Type: | Metabolomics to elucidate signaling pathway |
Project Summary: | Early detection of predators is critical to the survival of all living organisms. For phytoplankton, recognition and response to chemical cues from predators, as evidence of predation risk, is particularly crucial. The phytoplankton Alexandrium minutum upregulates its toxicity when exposed to copepodamides, a suite of compounds released by copepod predators. However, how A. minutum perceives these predatory cues and what metabolic pathways are involved in initiating toxin induction remains unknown. In this study LC/MS and NMR-based metabolomics uncovered subtle physiological responses of A. minutum to copepodamides, including dysregulation of valine biosynthesis and enhancement of butanoate metabolism and arginine biosynthesis. While we have yet to identify a chemoreceptor directly activated by copepod cues, based on the results of inhibition experiments detection of copepodamides appears to disrupt the activity of serine/threonine phosphatases leading to increased jasmonic acid biosynthesis and signaling, which leads to amplified gonyautoxin biosynthesis in A. minutum. This study is an important step toward a better understanding of chemosensory ecology of predator-prey interactions in phytoplankton. |
Institute: | Georgia Institute of Technology |
Department: | School of Biological Sciences, School of Chemistry and Biochemistry, Center for Microbial Dynamics and Infection, Parker H. Petit Institute for Bioengineering and Bioscience |
Laboratory: | Kubanek Lab |
Last Name: | Brown |
First Name: | Emily |
Address: | 950 Atlantic Dr Atlanta, GA, 30332, USA |
Email: | julia.kubanek@biosci.gatech.edu |
Phone: | 404-894-8424 |
Project Comments: | This study has 3 parts: 2 NMR (polar and non-polar metabolites) and MS |
Contributors: | Emily R. Brown, Sam G. Moore, David A. Gaul, and Julia Kubanek |
Subject:
Subject ID: | SU001959 |
Subject Type: | Other organism |
Subject Species: | Alexandrium minutum |
Species Group: | Dinoflagellates |
Factors:
Subject type: Other organism; Subject species: Alexandrium minutum (Factor headings shown in green)
mb_sample_id | local_sample_id | Factor |
---|---|---|
SA175001 | Aq_COP_08 | Copepodamide exposure |
SA175002 | Aq_COP_09 | Copepodamide exposure |
SA175003 | Aq_COP_10 | Copepodamide exposure |
SA175004 | Aq_COP_07 | Copepodamide exposure |
SA175005 | Aq_COP_04 | Copepodamide exposure |
SA175006 | Aq_COP_02 | Copepodamide exposure |
SA175007 | Aq_COP_03 | Copepodamide exposure |
SA175008 | Aq_COP_11 | Copepodamide exposure |
SA175009 | Aq_COP_06 | Copepodamide exposure |
SA175010 | Aq_COP_13 | Copepodamide exposure |
SA175011 | Aq_COP_18 | Copepodamide exposure |
SA175012 | Aq_COP_19 | Copepodamide exposure |
SA175013 | Aq_COP_20 | Copepodamide exposure |
SA175014 | Aq_COP_17 | Copepodamide exposure |
SA175015 | Aq_COP_16 | Copepodamide exposure |
SA175016 | Aq_COP_01 | Copepodamide exposure |
SA175017 | Aq_COP_14 | Copepodamide exposure |
SA175018 | Aq_COP_15 | Copepodamide exposure |
SA175019 | Aq_COP_12 | Copepodamide exposure |
SA175020 | Aq_COP_05 | Copepodamide exposure |
SA175030 | Aq_Control_05 | no exposure |
SA175031 | Aq_Control_04 | no exposure |
SA175032 | Aq_Control_02 | no exposure |
SA175033 | Aq_Control_06 | no exposure |
SA175034 | Aq_Control_03 | no exposure |
SA175035 | Aq_Control_07 | no exposure |
SA175036 | Aq_Control_16 | no exposure |
SA175037 | Aq_Control_15 | no exposure |
SA175038 | Aq_Control_17 | no exposure |
SA175039 | Aq_Control_01 | no exposure |
SA175040 | Aq_Control_19 | no exposure |
SA175041 | Aq_Control_18 | no exposure |
SA175042 | Aq_Control_14 | no exposure |
SA175043 | Aq_Control_13 | no exposure |
SA175044 | Aq_Control_09 | no exposure |
SA175045 | Aq_Control_08 | no exposure |
SA175046 | Aq_Control_10 | no exposure |
SA175047 | Aq_Control_11 | no exposure |
SA175048 | Aq_Control_12 | no exposure |
SA175049 | Aq_Control_20 | no exposure |
SA175021 | Quality_Control_02 | QC |
SA175022 | Quality_Control_01 | QC |
SA175023 | Blank_SB_06 | QC |
SA175024 | Blank_SB_05 | QC |
SA175025 | Quality_Control_04 | QC |
SA175026 | Quality_Control_03 | QC |
SA175027 | Quality_Control_06 | QC |
SA175028 | Quality_Control_07 | QC |
SA175029 | Quality_Control_05 | QC |
Showing results 1 to 49 of 49 |
Collection:
Collection ID: | CO001952 |
Collection Summary: | From culture (alexandrium minutum strain CCMP 113) |
Sample Type: | Algae |
Treatment:
Treatment ID: | TR001971 |
Treatment Summary: | Copepodamide que |
Sample Preparation:
Sampleprep ID: | SP001965 |
Sampleprep Summary: | Modified Bligh Dyer |
Combined analysis:
Analysis ID | AN003042 | AN003043 | AN003044 | AN003045 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
Column | Waters Acquity BEH HILIC (150 x 2.1mm,1.7um) | Waters Acquity BEH HILIC (150 x 2.1mm,1.7um) | Thermo Accucore C30 (150 x 2.1mm,2.6um) | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbritrap/ion trap | Orbritrap/ion trap | Orbitrap | Orbitrap |
MS instrument name | Thermo Orbitrap ID-X Tribrid | Thermo Orbitrap ID-X Tribrid | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | peak area | peak area | peak area | peak area |
Chromatography:
Chromatography ID: | CH002255 |
Chromatography Summary: | Polar HILIC separation |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters Acquity BEH HILIC (150 x 2.1mm,1.7um) |
Chromatography Type: | HILIC |
Chromatography ID: | CH002256 |
Chromatography Summary: | non-Polar reverse phase separation |
Instrument Name: | Thermo Vanquish |
Column Name: | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002830 |
Analysis ID: | AN003042 |
Instrument Name: | Thermo Orbitrap ID-X Tribrid |
Instrument Type: | Orbritrap/ion trap |
MS Type: | ESI |
MS Comments: | Compound Discoverer |
Ion Mode: | POSITIVE |
MS ID: | MS002831 |
Analysis ID: | AN003043 |
Instrument Name: | Thermo Orbitrap ID-X Tribrid |
Instrument Type: | Orbritrap/ion trap |
MS Type: | ESI |
MS Comments: | Compound Discoverer |
Ion Mode: | NEGATIVE |
MS ID: | MS002832 |
Analysis ID: | AN003044 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Compound Discoverer |
Ion Mode: | POSITIVE |
MS ID: | MS002833 |
Analysis ID: | AN003045 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Compound Discoverer |
Ion Mode: | NEGATIVE |