Summary of Study ST002193

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001397. The data can be accessed directly via it's Project DOI: 10.21228/M84T4X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002193
Study TitleThe effects of obesity microbiota produced metabolites on colorectal carcinogenesis in murine models
Study SummaryObesity is a risk factor for colorectal cancer (CRC). We aim to study the effects and mechanisms of gut microbiota of obese subjects in contributing to CRC progression. Conventional AOM-treated and ApcMin/+ mice receiving feces from obese individuals showed significantly increased colon tumor formation compared with those receiving feces from control subjects. AOM-treated mice receiving feces from obese (OB-M) exhibited microbiota dysbiosis with enriched potential pathobionts Erysipelotrichaceae bacterium GAM147, Turicibacter sp. H121, Mucinivorans hirudinis, and depleted symbionts Bacteroides vulgatus, Faecalibaculum rodentium, Bifidobacterium spp. and Lactobacillus delbrueckii. The OB-M group also showed altered gut metabolites including elevated phenylacetic acid, and depleted genipin. Moreover, OB-M group showed impaired intestinal barrier function and significant upregulation of pro-inflammatory cytokines and activation of oncogenic Wnt signaling pathway. In conclusion, gut microbiota from obese individuals promotes colorectal carcinogenesis. Microbiota modulation in obese individuals may provide new insight into obesity-driven CRC prevention and therapy.
Institute
Chinese University of Hong Kong
Last NameKang
First NameXing
AddressRm806, Li Ka Shing Medical Science Building, PWH, Shatin, Hong Kong
Emailkangxing92@163.com
Phone93760832
Submit Date2022-05-18
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-05-18
Release Version1
Xing Kang Xing Kang
https://dx.doi.org/10.21228/M84T4X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001397
Project DOI:doi: 10.21228/M84T4X
Project Title:The effects of obesity microbiota on colorectal carcinogenesis in murine models
Project Summary:Obesity is a risk factor for colorectal cancer (CRC). We aim to study the effects and mechanisms of gut microbiota of obese subjects in contributing to CRC progression. Conventional AOM-treated and ApcMin/+ mice receiving feces from obese individuals showed significantly increased colon tumor formation compared with those receiving feces from control subjects. AOM-treated mice receiving feces from obese (OB-M) exhibited microbiota dysbiosis with enriched potential pathobionts Erysipelotrichaceae bacterium GAM147, Turicibacter sp. H121, Mucinivorans hirudinis, and depleted symbionts Bacteroides vulgatus, Faecalibaculum rodentium, Bifidobacterium spp. and Lactobacillus delbrueckii. The OB-M group also showed altered gut metabolites including elevated phenylacetic acid, and depleted genipin. Moreover, OB-M group showed impaired intestinal barrier function and significant upregulation of pro-inflammatory cytokines and activation of oncogenic Wnt signaling pathway. In conclusion, gut microbiota from obese individuals promotes colorectal carcinogenesis. Microbiota modulation in obese individuals may provide new insight into obesity-driven CRC prevention and therapy.
Institute:The Chinese University of Hong Kong
Department:Medicine and theraputics
Last Name:Kang
First Name:Xing
Address:Rm806, Li Ka Shing Medical Science Building, PWH, Shatin, Hong Kong
Email:kangxing92@163.com
Phone:93760832

Subject:

Subject ID:SU002279
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA210265POS_2172LN-M
SA210266NEG_2162LN-M
SA210267POS_2178LN-M
SA210268POS_2179LN-M
SA210269POS_2193LN-M
SA210270NEG_2193LN-M
SA210271POS_2162LN-M
SA210272NEG_2172LN-M
SA210273NEG_2179LN-M
SA210274NEG_2178LN-M
SA210275POS_2187OB-M
SA210276NEG_2195OB-M
SA210277POS_2195OB-M
SA210278NEG_2164OB-M
SA210279POS_2181OB-M
SA210280NEG_2177OB-M
SA210281NEG_2181OB-M
SA210282POS_2177OB-M
SA210283NEG_2187OB-M
SA210284POS_2164OB-M
Showing results 1 to 20 of 20

Collection:

Collection ID:CO002272
Collection Summary:Mice stool samples were snap frozen in liquid nitrogen and kept at −80°C until further use
Sample Type:Feces

Treatment:

Treatment ID:TR002291
Treatment Summary:AOM-induced mice were treated with feces from normal BMI and obese individuals.

Sample Preparation:

Sampleprep ID:SP002285
Sampleprep Summary:25 mg of each fecal sample from AOM-induced mice was added into 500 μL extract solution (methanol: acetonitrile: water = 2:2:1) and homogenized.

Chromatography:

Chromatography ID:CH002652
Instrument Name:Thermo Q Exactive HF-X
Column Name:UPLC BEH Amide column (2.1mm × 100mm, 1.7μm)
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN003589
Analysis Type:MS
Chromatography ID:CH002652
Has Mz:1
Has Rt:1
Rt Units:Seconds
Results File:ST002193_AN003589_Results.txt
Units:m/z
  
Analysis ID:AN003590
Analysis Type:MS
Chromatography ID:CH002652
Has Mz:1
Has Rt:1
Rt Units:Seconds
Results File:ST002193_AN003590_Results.txt
Units:m/z
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