Summary of Study ST002193

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001397. The data can be accessed directly via it's Project DOI: 10.21228/M84T4X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002193
Study TitleThe effects of obesity microbiota produced metabolites on colorectal carcinogenesis in murine models
Study SummaryObesity is a risk factor for colorectal cancer (CRC). We aim to study the effects and mechanisms of gut microbiota of obese subjects in contributing to CRC progression. Conventional AOM-treated and ApcMin/+ mice receiving feces from obese individuals showed significantly increased colon tumor formation compared with those receiving feces from control subjects. AOM-treated mice receiving feces from obese (OB-M) exhibited microbiota dysbiosis with enriched potential pathobionts Erysipelotrichaceae bacterium GAM147, Turicibacter sp. H121, Mucinivorans hirudinis, and depleted symbionts Bacteroides vulgatus, Faecalibaculum rodentium, Bifidobacterium spp. and Lactobacillus delbrueckii. The OB-M group also showed altered gut metabolites including elevated phenylacetic acid, and depleted genipin. Moreover, OB-M group showed impaired intestinal barrier function and significant upregulation of pro-inflammatory cytokines and activation of oncogenic Wnt signaling pathway. In conclusion, gut microbiota from obese individuals promotes colorectal carcinogenesis. Microbiota modulation in obese individuals may provide new insight into obesity-driven CRC prevention and therapy.
Institute
The Chinese University of Hong Kong
Last NameKang
First NameXing
AddressRm806, Li Ka Shing Medical Science Building, PWH, Shatin, Hong Kong
Emailkangxing92@163.com
Phone93760832
Submit Date2022-05-18
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-05-18
Release Version1
Xing Kang Xing Kang
https://dx.doi.org/10.21228/M84T4X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001397
Project DOI:doi: 10.21228/M84T4X
Project Title:The effects of obesity microbiota on colorectal carcinogenesis in murine models
Project Summary:Obesity is a risk factor for colorectal cancer (CRC). We aim to study the effects and mechanisms of gut microbiota of obese subjects in contributing to CRC progression. Conventional AOM-treated and ApcMin/+ mice receiving feces from obese individuals showed significantly increased colon tumor formation compared with those receiving feces from control subjects. AOM-treated mice receiving feces from obese (OB-M) exhibited microbiota dysbiosis with enriched potential pathobionts Erysipelotrichaceae bacterium GAM147, Turicibacter sp. H121, Mucinivorans hirudinis, and depleted symbionts Bacteroides vulgatus, Faecalibaculum rodentium, Bifidobacterium spp. and Lactobacillus delbrueckii. The OB-M group also showed altered gut metabolites including elevated phenylacetic acid, and depleted genipin. Moreover, OB-M group showed impaired intestinal barrier function and significant upregulation of pro-inflammatory cytokines and activation of oncogenic Wnt signaling pathway. In conclusion, gut microbiota from obese individuals promotes colorectal carcinogenesis. Microbiota modulation in obese individuals may provide new insight into obesity-driven CRC prevention and therapy.
Institute:The Chinese University of Hong Kong
Department:Medicine and theraputics
Last Name:Kang
First Name:Xing
Address:Rm806, Li Ka Shing Medical Science Building, PWH, Shatin, Hong Kong
Email:kangxing92@163.com
Phone:93760832

Subject:

Subject ID:SU002279
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA210265POS_2172LN-M
SA210266NEG_2162LN-M
SA210267POS_2178LN-M
SA210268POS_2179LN-M
SA210269POS_2193LN-M
SA210270NEG_2193LN-M
SA210271POS_2162LN-M
SA210272NEG_2172LN-M
SA210273NEG_2179LN-M
SA210274NEG_2178LN-M
SA210275POS_2187OB-M
SA210276NEG_2195OB-M
SA210277POS_2195OB-M
SA210278NEG_2164OB-M
SA210279POS_2181OB-M
SA210280NEG_2177OB-M
SA210281NEG_2181OB-M
SA210282POS_2177OB-M
SA210283NEG_2187OB-M
SA210284POS_2164OB-M
Showing results 1 to 20 of 20

Collection:

Collection ID:CO002272
Collection Summary:Mice stool samples were snap frozen in liquid nitrogen and kept at −80°C until further use
Sample Type:Feces

Treatment:

Treatment ID:TR002291
Treatment Summary:AOM-induced mice were treated with feces from normal BMI and obese individuals.

Sample Preparation:

Sampleprep ID:SP002285
Sampleprep Summary:25 mg of each fecal sample from AOM-induced mice was added into 500 μL extract solution (methanol: acetonitrile: water = 2:2:1) and homogenized.

Combined analysis:

Analysis ID AN003589 AN003590
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Q Exactive HF-X Thermo Q Exactive HF-X
Column UPLC BEH Amide column (2.1mm × 100mm, 1.7μm) UPLC BEH Amide column (2.1mm × 100mm, 1.7μm)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE NEGATIVE
Units m/z m/z

Chromatography:

Chromatography ID:CH002652
Instrument Name:Thermo Q Exactive HF-X
Column Name:UPLC BEH Amide column (2.1mm × 100mm, 1.7μm)
Chromatography Type:Reversed phase

MS:

MS ID:MS003344
Analysis ID:AN003589
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:After centrifuge at 12000 rpm for 15 min at 4℃, the supernatant was transferred to an UHPLC system (Vanquish, Thermo Fisher Scientific) with a UPLC BEH Amide column (2.1mm × 100mm, 1.7μm) coupled to Q Exactive HFX mass spectrometer (Orbitrap MS, Thermo) for LC-MS/MS analyses. The mass spectrometer was operated on information dependent acquisition (IDA) mode in the control of the acquisition software (Xcalibur, Thermo). Raw data was converted to mzXML format using ProteoWizard and processed with an in-house program, which was developed using R and based on XCMS, for peak detection, extraction, alignment, and integration.
Ion Mode:POSITIVE
  
MS ID:MS003345
Analysis ID:AN003590
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:After centrifuge at 12000 rpm for 15 min at 4℃, the supernatant was transferred to an UHPLC system (Vanquish, Thermo Fisher Scientific) with a UPLC BEH Amide column (2.1mm × 100mm, 1.7μm) coupled to Q Exactive HFX mass spectrometer (Orbitrap MS, Thermo) for LC-MS/MS analyses. The mass spectrometer was operated on information dependent acquisition (IDA) mode in the control of the acquisition software (Xcalibur, Thermo). Raw data was converted to mzXML format using ProteoWizard and processed with an in-house program, which was developed using R and based on XCMS, for peak detection, extraction, alignment, and integration.
Ion Mode:NEGATIVE
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