Summary of Study ST002324

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001490. The data can be accessed directly via it's Project DOI: 10.21228/M84D89 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002324
Study TitleExploration of age-dependent changes of phospholipid profiles in C. elegans depleted of tif-IA and ncl-1
Study SummaryAnalysis of phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylserines and phosphatidylglycerols in young (d2), middle age (d6) and old age (d12) C. elegans as well as animals with tif-IA or ncl-1 knockdown.
Institute
University of Innsbruck
DepartmentMichael Popp Institute
Last NameKoeberle
First NameAndreas
AddressMitterweg 24, Innsbruck, Tyrol, 6020, Austria
Emailandreas.koeberle@uibk.ac.at
Phone+43 512 507 57903
Submit Date2022-10-12
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2022-11-18
Release Version1
Andreas Koeberle Andreas Koeberle
https://dx.doi.org/10.21228/M84D89
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001490
Project DOI:doi: 10.21228/M84D89
Project Title:Reducing the metabolic burden of rRNA synthesis promotes healthy longevity
Project Summary:Ribosome biogenesis is an anabolic process driven by RNA polymerase I (Pol I)-mediated synthesis of pre-ribosomal RNA (pre-rRNA). While Pol I activity was previously linked to longevity, the underlying mechanisms were not studied beyond effects on protein translation and downstream proteostasis. Here we used multi-omics and functional tests to show that curtailment of Pol I activity preserves mitochondrial function and lowers ATP expenditure, thereby promoting longevity in Caenorhabditis elegans. Reduced pre-rRNA synthesis also improved longevity and energy homeostasis in Drosophila melanogaster and human cells, respectively. Conversely, the enhancement of pre-rRNA synthesis boosted growth and neuromuscular performance of young nematodes at the cost of accelerated metabolic decline, mitochondrial stress and premature aging. Moreover, restriction of Pol I activity extended lifespan more potently than translational repression, and retained its geroprotective effects when initiated late in life, showcasing moderation of Pol I activity as an effective longevity treatment not limited by aging.
Institute:University of Innsbruck
Department:Michael Popp Institute
Last Name:Koeberle
First Name:Andreas
Address:Mitterweg 24, Innsbruck, Tyrol, 6020, Austria
Email:andreas.koeberle@uibk.ac.at
Phone:+43 512 507 57903
Funding Source:Leibniz Association, Thüringer Aufbaubank (2019 FGR 0082), Free State of Thuringia (RegenerAging—FSU-I-03/14), Carl-Zeiss-Stiftung (IMPULS), Phospholipid Research Center (AKO-2019-070/2-1)
Contributors:Samim Sharifi, Finja Witt, André Gollowitzer, Oliver Werz, Holger Bierhoff, Maria Ermolaeva

Subject:

Subject ID:SU002411
Subject Type:Invertebrate
Subject Species:Caenorhabditis elegans
Taxonomy ID:6239
Age Or Age Range:Young, middle aged and old

Factors:

Subject type: Invertebrate; Subject species: Caenorhabditis elegans (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Days of adulthood
SA229091191115_FW_1_project_Ermolaeva_1_10_PE030ctrl RNAi 12
SA229092191115_FW_1_project_Ermolaeva_1_10_PE039ctrl RNAi 12
SA229093191115_FW_1_project_Ermolaeva_1_10_PI003ctrl RNAi 12
SA229094191115_FW_1_project_Ermolaeva_1_10_PI021ctrl RNAi 12
SA229095191115_FW_1_project_Ermolaeva_1_10_PG039ctrl RNAi 12
SA229096191115_FW_1_project_Ermolaeva_1_10_PE021ctrl RNAi 12
SA229097191115_FW_1_project_Ermolaeva_phosphoPC039ctrl RNAi 12
SA229098191115_FW_1_project_Ermolaeva_1_10_PE003ctrl RNAi 12
SA229099191115_FW_1_project_Ermolaeva_1_10_PE012ctrl RNAi 12
SA229100191115_FW_1_project_Ermolaeva_1_10_PI030ctrl RNAi 12
SA229101191115_FW_1_project_Ermolaeva_1_10_PS003ctrl RNAi 12
SA229102191115_FW_1_project_Ermolaeva_1_10_PS039ctrl RNAi 12
SA229103191115_FW_1_project_Ermolaeva_1_10_PG003ctrl RNAi 12
SA229104191115_FW_1_project_Ermolaeva_1_10_PG012ctrl RNAi 12
SA229105191115_FW_1_project_Ermolaeva_1_10_PG021ctrl RNAi 12
SA229106191115_FW_1_project_Ermolaeva_1_10_PS030ctrl RNAi 12
SA229107191115_FW_1_project_Ermolaeva_1_10_PG030ctrl RNAi 12
SA229108191115_FW_1_project_Ermolaeva_phosphoPC030ctrl RNAi 12
SA229109191115_FW_1_project_Ermolaeva_1_10_PS012ctrl RNAi 12
SA229110191115_FW_1_project_Ermolaeva_1_10_PS021ctrl RNAi 12
SA229111191115_FW_1_project_Ermolaeva_1_10_PI039ctrl RNAi 12
SA229112191115_FW_1_project_Ermolaeva_1_10_PI012ctrl RNAi 12
SA229113191115_FW_1_project_Ermolaeva_phosphoPC003ctrl RNAi 12
SA229114191115_FW_1_project_Ermolaeva_phosphoPC021ctrl RNAi 12
SA229115191115_FW_1_project_Ermolaeva_phosphoPC012ctrl RNAi 12
SA229116191115_FW_1_project_Ermolaeva_1_10_PI001ctrl RNAi 2
SA229117191115_FW_1_project_Ermolaeva_1_10_PI010ctrl RNAi 2
SA229118191115_FW_1_project_Ermolaeva_1_10_PI019ctrl RNAi 2
SA229119191115_FW_1_project_Ermolaeva_1_10_PG037ctrl RNAi 2
SA229120191115_FW_1_project_Ermolaeva_1_10_PE037ctrl RNAi 2
SA229121191115_FW_1_project_Ermolaeva_1_10_PE010ctrl RNAi 2
SA229122191115_FW_1_project_Ermolaeva_1_10_PE019ctrl RNAi 2
SA229123191115_FW_1_project_Ermolaeva_phosphoPC019ctrl RNAi 2
SA229124191115_FW_1_project_Ermolaeva_1_10_PE028ctrl RNAi 2
SA229125191115_FW_1_project_Ermolaeva_1_10_PI028ctrl RNAi 2
SA229126191115_FW_1_project_Ermolaeva_1_10_PS001ctrl RNAi 2
SA229127191115_FW_1_project_Ermolaeva_1_10_PG028ctrl RNAi 2
SA229128191115_FW_1_project_Ermolaeva_1_10_PG001ctrl RNAi 2
SA229129191115_FW_1_project_Ermolaeva_1_10_PG010ctrl RNAi 2
SA229130191115_FW_1_project_Ermolaeva_1_10_PG019ctrl RNAi 2
SA229131191115_FW_1_project_Ermolaeva_1_10_PS037ctrl RNAi 2
SA229132191115_FW_1_project_Ermolaeva_1_10_PS028ctrl RNAi 2
SA229133191115_FW_1_project_Ermolaeva_1_10_PS010ctrl RNAi 2
SA229134191115_FW_1_project_Ermolaeva_phosphoPC010ctrl RNAi 2
SA229135191115_FW_1_project_Ermolaeva_1_10_PS019ctrl RNAi 2
SA229136191115_FW_1_project_Ermolaeva_1_10_PI037ctrl RNAi 2
SA229137191115_FW_1_project_Ermolaeva_phosphoPC001ctrl RNAi 2
SA229138191115_FW_1_project_Ermolaeva_phosphoPC037ctrl RNAi 2
SA229139191115_FW_1_project_Ermolaeva_phosphoPC028ctrl RNAi 2
SA229140191115_FW_1_project_Ermolaeva_1_10_PE001ctrl RNAi 2
SA229141191115_FW_1_project_Ermolaeva_1_10_PS020ctrl RNAi 6
SA229142191115_FW_1_project_Ermolaeva_1_10_PI029ctrl RNAi 6
SA229143191115_FW_1_project_Ermolaeva_phosphoPC038ctrl RNAi 6
SA229144191115_FW_1_project_Ermolaeva_1_10_PS029ctrl RNAi 6
SA229145191115_FW_1_project_Ermolaeva_1_10_PG029ctrl RNAi 6
SA229146191115_FW_1_project_Ermolaeva_1_10_PE029ctrl RNAi 6
SA229147191115_FW_1_project_Ermolaeva_1_10_PS011ctrl RNAi 6
SA229148191115_FW_1_project_Ermolaeva_1_10_PS002ctrl RNAi 6
SA229149191115_FW_1_project_Ermolaeva_1_10_PE038ctrl RNAi 6
SA229150191115_FW_1_project_Ermolaeva_phosphoPC011ctrl RNAi 6
SA229151191115_FW_1_project_Ermolaeva_1_10_PG038ctrl RNAi 6
SA229152191115_FW_1_project_Ermolaeva_phosphoPC002ctrl RNAi 6
SA229153191115_FW_1_project_Ermolaeva_1_10_PE020ctrl RNAi 6
SA229154191115_FW_1_project_Ermolaeva_1_10_PS038ctrl RNAi 6
SA229155191115_FW_1_project_Ermolaeva_1_10_PE011ctrl RNAi 6
SA229156191115_FW_1_project_Ermolaeva_phosphoPC020ctrl RNAi 6
SA229157191115_FW_1_project_Ermolaeva_1_10_PE002ctrl RNAi 6
SA229158191115_FW_1_project_Ermolaeva_1_10_PI002ctrl RNAi 6
SA229159191115_FW_1_project_Ermolaeva_1_10_PG011ctrl RNAi 6
SA229160191115_FW_1_project_Ermolaeva_1_10_PI011ctrl RNAi 6
SA229161191115_FW_1_project_Ermolaeva_1_10_PG002ctrl RNAi 6
SA229162191115_FW_1_project_Ermolaeva_1_10_PI038ctrl RNAi 6
SA229163191115_FW_1_project_Ermolaeva_phosphoPC029ctrl RNAi 6
SA229164191115_FW_1_project_Ermolaeva_1_10_PI020ctrl RNAi 6
SA229165191115_FW_1_project_Ermolaeva_1_10_PG020ctrl RNAi 6
SA229166191115_FW_1_project_Ermolaeva_1_10_PG045ncl-1 RNAi 12
SA229167191115_FW_1_project_Ermolaeva_1_10_PI018ncl-1 RNAi 12
SA229168191115_FW_1_project_Ermolaeva_1_10_PI009ncl-1 RNAi 12
SA229169191115_FW_1_project_Ermolaeva_1_10_PI027ncl-1 RNAi 12
SA229170191115_FW_1_project_Ermolaeva_phosphoPC009ncl-1 RNAi 12
SA229171191115_FW_1_project_Ermolaeva_1_10_PG027ncl-1 RNAi 12
SA229172191115_FW_1_project_Ermolaeva_1_10_PS045ncl-1 RNAi 12
SA229173191115_FW_1_project_Ermolaeva_1_10_PG009ncl-1 RNAi 12
SA229174191115_FW_1_project_Ermolaeva_phosphoPC027ncl-1 RNAi 12
SA229175191115_FW_1_project_Ermolaeva_1_10_PG018ncl-1 RNAi 12
SA229176191115_FW_1_project_Ermolaeva_1_10_PS036ncl-1 RNAi 12
SA229177191115_FW_1_project_Ermolaeva_1_10_PS027ncl-1 RNAi 12
SA229178191115_FW_1_project_Ermolaeva_1_10_PI045ncl-1 RNAi 12
SA229179191115_FW_1_project_Ermolaeva_1_10_PS009ncl-1 RNAi 12
SA229180191115_FW_1_project_Ermolaeva_1_10_PS018ncl-1 RNAi 12
SA229181191115_FW_1_project_Ermolaeva_1_10_PG036ncl-1 RNAi 12
SA229182191115_FW_1_project_Ermolaeva_phosphoPC036ncl-1 RNAi 12
SA229183191115_FW_1_project_Ermolaeva_1_10_PI036ncl-1 RNAi 12
SA229184191115_FW_1_project_Ermolaeva_1_10_PE027ncl-1 RNAi 12
SA229185191115_FW_1_project_Ermolaeva_1_10_PE036ncl-1 RNAi 12
SA229186191115_FW_1_project_Ermolaeva_1_10_PE018ncl-1 RNAi 12
SA229187191115_FW_1_project_Ermolaeva_1_10_PE045ncl-1 RNAi 12
SA229188191115_FW_1_project_Ermolaeva_phosphoPC018ncl-1 RNAi 12
SA229189191115_FW_1_project_Ermolaeva_phosphoPC045ncl-1 RNAi 12
SA229190191115_FW_1_project_Ermolaeva_1_10_PE009ncl-1 RNAi 12
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Collection:

Collection ID:CO002404
Collection Summary:Worms were washed and pelleted, M9 media was removed with ~100 µl remaining in the sample, and the sample was snap frozen.
Sample Type:Worms

Treatment:

Treatment ID:TR002423
Treatment Summary:Ncl-1 and tif-IA knockdown was achieved by feeding the worms for 2 generations with bacteria (from the Ahringer RNAi feeding library, Source BioScience Ltd.) producing the corresponding dsRNA. Worms were cultured under standard conditions as described in ‘Wormbook’, F1 was grown at 15°C and F2 at 20°C, and F2 animals were grown on RNAi until days 2, 6 or 12 of adulthood.

Sample Preparation:

Sampleprep ID:SP002417
Sampleprep Summary:Phospholipids were extracted from C. elegans by successive addition of PBS pH 7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17. Evaporation of the organic layer yielded a lipid film that was dissolved in methanol and subjected to UPLC-MS/MS.
Extract Storage:-20℃

Combined analysis:

Analysis ID AN003793
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity H-Class
Column Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap
Ion Mode NEGATIVE
Units relative units

Chromatography:

Chromatography ID:CH002806
Chromatography Summary:Chromatographic separation of phospholipids was carried out on an Acquity BEH C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an Acquity UHPLC.
Instrument Name:Waters Acquity H-Class
Column Name:Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS003535
Analysis ID:AN003793
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Targeted MRM with pre-optimized settings and subsequent automated integration of selected signals using Analyst 1.6.2 or Analyst 1.6.3 (Sciex).
Ion Mode:NEGATIVE
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