Summary of Study ST002325

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001490. The data can be accessed directly via it's Project DOI: 10.21228/M84D89 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002325
Study TitleDetermination of changes in free fatty acid levels in C. elegans depleted of tif-IA and ncl-1
Study SummaryAnalysis of free fatty acids in young (d2), middle age (d6) and old age (d12) C. elegans with tif-IA or ncl-1 knockdown.
Institute
University of Innsbruck
DepartmentMichael Popp Institute
Last NameKoeberle
First NameAndreas
AddressMitterweg 24, Innsbruck, Tyrol, 6020, Austria
Emailandreas.koeberle@uibk.ac.at
Phone+43 512 507 57903
Submit Date2022-10-12
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2022-11-18
Release Version1
Andreas Koeberle Andreas Koeberle
https://dx.doi.org/10.21228/M84D89
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001490
Project DOI:doi: 10.21228/M84D89
Project Title:Reducing the metabolic burden of rRNA synthesis promotes healthy longevity
Project Summary:Ribosome biogenesis is an anabolic process driven by RNA polymerase I (Pol I)-mediated synthesis of pre-ribosomal RNA (pre-rRNA). While Pol I activity was previously linked to longevity, the underlying mechanisms were not studied beyond effects on protein translation and downstream proteostasis. Here we used multi-omics and functional tests to show that curtailment of Pol I activity preserves mitochondrial function and lowers ATP expenditure, thereby promoting longevity in Caenorhabditis elegans. Reduced pre-rRNA synthesis also improved longevity and energy homeostasis in Drosophila melanogaster and human cells, respectively. Conversely, the enhancement of pre-rRNA synthesis boosted growth and neuromuscular performance of young nematodes at the cost of accelerated metabolic decline, mitochondrial stress and premature aging. Moreover, restriction of Pol I activity extended lifespan more potently than translational repression, and retained its geroprotective effects when initiated late in life, showcasing moderation of Pol I activity as an effective longevity treatment not limited by aging.
Institute:University of Innsbruck
Department:Michael Popp Institute
Last Name:Koeberle
First Name:Andreas
Address:Mitterweg 24, Innsbruck, Tyrol, 6020, Austria
Email:andreas.koeberle@uibk.ac.at
Phone:+43 512 507 57903
Funding Source:Leibniz Association, Thüringer Aufbaubank (2019 FGR 0082), Free State of Thuringia (RegenerAging—FSU-I-03/14), Carl-Zeiss-Stiftung (IMPULS), Phospholipid Research Center (AKO-2019-070/2-1)
Contributors:Samim Sharifi, Finja Witt, André Gollowitzer, Oliver Werz, Holger Bierhoff, Maria Ermolaeva

Subject:

Subject ID:SU002412
Subject Type:Invertebrate
Subject Species:Caenorhabditis elegans
Taxonomy ID:6239
Age Or Age Range:Young, middle aged and old

Factors:

Subject type: Invertebrate; Subject species: Caenorhabditis elegans (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Days of adulthood
SA229316200301_Ermoleva_FFA_12ctrl RNAi 12
SA229317200301_Ermoleva_FFA_39ctrl RNAi 12
SA229318200301_Ermoleva_FFA_30ctrl RNAi 12
SA229319200301_Ermoleva_FFA_21ctrl RNAi 12
SA229320200301_Ermoleva_FFA_3ctrl RNAi 12
SA229321200301_Ermoleva_FFA_28ctrl RNAi 2
SA229322200301_Ermoleva_FFA_37ctrl RNAi 2
SA229323200301_Ermoleva_FFA_19ctrl RNAi 2
SA229324200301_Ermoleva_FFA_1ctrl RNAi 2
SA229325200301_Ermoleva_FFA_10ctrl RNAi 2
SA229326200301_Ermoleva_FFA_11ctrl RNAi 6
SA229327200301_Ermoleva_FFA_29ctrl RNAi 6
SA229328200301_Ermoleva_FFA_38ctrl RNAi 6
SA229329200301_Ermoleva_FFA_20ctrl RNAi 6
SA229330200301_Ermoleva_FFA_2ctrl RNAi 6
SA229331200301_Ermoleva_FFA_9ncl-1 RNAi 12
SA229332200301_Ermoleva_FFA_27ncl-1 RNAi 12
SA229333200301_Ermoleva_FFA_36ncl-1 RNAi 12
SA229334200301_Ermoleva_FFA_18ncl-1 RNAi 12
SA229335200301_Ermoleva_FFA_45ncl-1 RNAi 12
SA229336200301_Ermoleva_FFA_25ncl-1 RNAi 2
SA229337200301_Ermoleva_FFA_7ncl-1 RNAi 2
SA229338200301_Ermoleva_FFA_34ncl-1 RNAi 2
SA229339200301_Ermoleva_FFA_16ncl-1 RNAi 2
SA229340200301_Ermoleva_FFA_43ncl-1 RNAi 2
SA229341200301_Ermoleva_FFA_35ncl-1 RNAi 6
SA229342200301_Ermoleva_FFA_8ncl-1 RNAi 6
SA229343200301_Ermoleva_FFA_44ncl-1 RNAi 6
SA229344200301_Ermoleva_FFA_26ncl-1 RNAi 6
SA229345200301_Ermoleva_FFA_17ncl-1 RNAi 6
SA229346200301_Ermoleva_FFA_15tif-IA RNAi 12
SA229347200301_Ermoleva_FFA_6tif-IA RNAi 12
SA229348200301_Ermoleva_FFA_42tif-IA RNAi 12
SA229349200301_Ermoleva_FFA_33tif-IA RNAi 12
SA229350200301_Ermoleva_FFA_24tif-IA RNAi 12
SA229351200301_Ermoleva_FFA_4tif-IA RNAi 2
SA229352200301_Ermoleva_FFA_40tif-IA RNAi 2
SA229353200301_Ermoleva_FFA_13tif-IA RNAi 2
SA229354200301_Ermoleva_FFA_22tif-IA RNAi 2
SA229355200301_Ermoleva_FFA_31tif-IA RNAi 2
SA229356200301_Ermoleva_FFA_23tif-IA RNAi 6
SA229357200301_Ermoleva_FFA_5tif-IA RNAi 6
SA229358200301_Ermoleva_FFA_14tif-IA RNAi 6
SA229359200301_Ermoleva_FFA_32tif-IA RNAi 6
SA229360200301_Ermoleva_FFA_41tif-IA RNAi 6
Showing results 1 to 45 of 45

Collection:

Collection ID:CO002405
Collection Summary:Worms were washed and pelleted, M9 media was removed with ~100µl remaining in the sample, and the sample was snap frozen.
Sample Type:Worms

Treatment:

Treatment ID:TR002424
Treatment Summary:Ncl-1 and tif-IA knockdown was achieved by feeding the worms for 2 generations with bacteria (from the Ahringer RNAi feeding library, Source BioScience Ltd.) producing the corresponding dsRNA. Worms were cultured under standard conditions as described in ‘Wormbook’, F1 was grown at 15°C and F2 at 20°C, and F2 animals were grown on RNAi until days 2, 6 or 12 of adulthood.

Sample Preparation:

Sampleprep ID:SP002418
Sampleprep Summary:Free fatty acids were extracted from C. elegans by successive addition of PBS pH 7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17. Evaporation of the organic layer yielded a lipid film that was dissolved in methanol and subjected to UPLC-MS/MS.
Extract Storage:-20℃

Combined analysis:

Analysis ID AN003794
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity H-Class
Column Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap
Ion Mode NEGATIVE
Units relative units

Chromatography:

Chromatography ID:CH002807
Chromatography Summary:Chromatographic separation of free fatty acids was carried out on an Acquity BEH C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an Acquity UHPLC.
Instrument Name:Waters Acquity H-Class
Column Name:Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS003536
Analysis ID:AN003794
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Multiple ion monitoring with pre-optimized settings and subsequent automated integration of selected signals using Analyst 1.6.2 or Analyst 1.6.3 (Sciex).
Ion Mode:NEGATIVE
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