Summary of Study ST002326

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001490. The data can be accessed directly via it's Project DOI: 10.21228/M84D89 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002326
Study TitleAnalysis of age-dependent changes of triacylglycerol species in C. elegans depleted of tif-IA and ncl-1
Study SummaryAnalysis of triacylglycerol species in young (d2), middle age (d6) and old age (d12) C. elegans with tif-IA or ncl-1 knockdown.
Institute
University of Innsbruck
Last NameKoeberle
First NameAndreas
AddressMitterweg 24, Innsbruck, Tyrol, 6020, Austria
Emailandreas.koeberle@uibk.ac.at
Phone+43 512 507 57903
Submit Date2022-10-12
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2022-11-18
Release Version1
Andreas Koeberle Andreas Koeberle
https://dx.doi.org/10.21228/M84D89
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001490
Project DOI:doi: 10.21228/M84D89
Project Title:Reducing the metabolic burden of rRNA synthesis promotes healthy longevity
Project Summary:Ribosome biogenesis is an anabolic process driven by RNA polymerase I (Pol I)-mediated synthesis of pre-ribosomal RNA (pre-rRNA). While Pol I activity was previously linked to longevity, the underlying mechanisms were not studied beyond effects on protein translation and downstream proteostasis. Here we used multi-omics and functional tests to show that curtailment of Pol I activity preserves mitochondrial function and lowers ATP expenditure, thereby promoting longevity in Caenorhabditis elegans. Reduced pre-rRNA synthesis also improved longevity and energy homeostasis in Drosophila melanogaster and human cells, respectively. Conversely, the enhancement of pre-rRNA synthesis boosted growth and neuromuscular performance of young nematodes at the cost of accelerated metabolic decline, mitochondrial stress and premature aging. Moreover, restriction of Pol I activity extended lifespan more potently than translational repression, and retained its geroprotective effects when initiated late in life, showcasing moderation of Pol I activity as an effective longevity treatment not limited by aging.
Institute:University of Innsbruck
Department:Michael Popp Institute
Last Name:Koeberle
First Name:Andreas
Address:Mitterweg 24, Innsbruck, Tyrol, 6020, Austria
Email:andreas.koeberle@uibk.ac.at
Phone:+43 512 507 57903
Funding Source:Leibniz Association, Thüringer Aufbaubank (2019 FGR 0082), Free State of Thuringia (RegenerAging—FSU-I-03/14), Carl-Zeiss-Stiftung (IMPULS), Phospholipid Research Center (AKO-2019-070/2-1)
Contributors:Samim Sharifi, Finja Witt, André Gollowitzer, Oliver Werz, Holger Bierhoff, Maria Ermolaeva

Subject:

Subject ID:SU002413
Subject Type:Invertebrate
Subject Species:Caenorhabditis elegans
Taxonomy ID:6239
Age Or Age Range:Young, middle aged and old

Factors:

Subject type: Invertebrate; Subject species: Caenorhabditis elegans (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Days of adulthood
SA229361191111_FW_1_project_Ermolaeva021ctrl RNAi 12
SA229362191111_FW_1_project_Ermolaeva012_TAGIIctrl RNAi 12
SA229363191111_FW_1_project_Ermolaeva003_TAGIIctrl RNAi 12
SA229364191111_FW_1_project_Ermolaeva021_TAGIIctrl RNAi 12
SA229365191111_FW_1_project_Ermolaeva012ctrl RNAi 12
SA229366191111_FW_1_project_Ermolaeva030ctrl RNAi 12
SA229367191111_FW_1_project_Ermolaeva003ctrl RNAi 12
SA229368191111_FW_1_project_Ermolaeva039ctrl RNAi 12
SA229369191111_FW_1_project_Ermolaeva030_TAGIIctrl RNAi 12
SA229370191111_FW_1_project_Ermolaeva039_TAGIIctrl RNAi 12
SA229371191111_FW_1_project_Ermolaeva028ctrl RNAi 2
SA229372191111_FW_1_project_Ermolaeva037ctrl RNAi 2
SA229373191111_FW_1_project_Ermolaeva019_TAGIIctrl RNAi 2
SA229374191111_FW_1_project_Ermolaeva037_TAGIIctrl RNAi 2
SA229375191111_FW_1_project_Ermolaeva028_TAGIIctrl RNAi 2
SA229376191111_FW_1_project_Ermolaeva010_TAGIIctrl RNAi 2
SA229377191111_FW_1_project_Ermolaeva001ctrl RNAi 2
SA229378191111_FW_1_project_Ermolaeva001_TAGIIctrl RNAi 2
SA229379191111_FW_1_project_Ermolaeva010ctrl RNAi 2
SA229380191111_FW_1_project_Ermolaeva019ctrl RNAi 2
SA229381191111_FW_1_project_Ermolaeva011_TAGIIctrl RNAi 6
SA229382191111_FW_1_project_Ermolaeva002_TAGIIctrl RNAi 6
SA229383191111_FW_1_project_Ermolaeva020ctrl RNAi 6
SA229384191111_FW_1_project_Ermolaeva029_TAGIIctrl RNAi 6
SA229385191111_FW_1_project_Ermolaeva002ctrl RNAi 6
SA229386191111_FW_1_project_Ermolaeva038_TAGIIctrl RNAi 6
SA229387191111_FW_1_project_Ermolaeva038ctrl RNAi 6
SA229388191111_FW_1_project_Ermolaeva020_TAGIIctrl RNAi 6
SA229389191111_FW_1_project_Ermolaeva011ctrl RNAi 6
SA229390191111_FW_1_project_Ermolaeva029ctrl RNAi 6
SA229391191111_FW_1_project_Ermolaeva027_TAGIIncl-1 RNAi 12
SA229392191111_FW_1_project_Ermolaeva018ncl-1 RNAi 12
SA229393191111_FW_1_project_Ermolaeva027ncl-1 RNAi 12
SA229394191111_FW_1_project_Ermolaeva045ncl-1 RNAi 12
SA229395191111_FW_1_project_Ermolaeva009ncl-1 RNAi 12
SA229396191111_FW_1_project_Ermolaeva036ncl-1 RNAi 12
SA229397191111_FW_1_project_Ermolaeva045_TAGIIncl-1 RNAi 12
SA229398191111_FW_1_project_Ermolaeva036_TAGIIncl-1 RNAi 12
SA229399191111_FW_1_project_Ermolaeva009_TAGIIncl-1 RNAi 12
SA229400191111_FW_1_project_Ermolaeva018_TAGIIncl-1 RNAi 12
SA229401191111_FW_1_project_Ermolaeva025_TAGIIncl-1 RNAi 2
SA229402191111_FW_1_project_Ermolaeva043_TAGIIncl-1 RNAi 2
SA229403191111_FW_1_project_Ermolaeva034_TAGIIncl-1 RNAi 2
SA229404191111_FW_1_project_Ermolaeva007ncl-1 RNAi 2
SA229405191111_FW_1_project_Ermolaeva007_TAGIIncl-1 RNAi 2
SA229406191111_FW_1_project_Ermolaeva025ncl-1 RNAi 2
SA229407191111_FW_1_project_Ermolaeva016_TAGIIncl-1 RNAi 2
SA229408191111_FW_1_project_Ermolaeva034ncl-1 RNAi 2
SA229409191111_FW_1_project_Ermolaeva043ncl-1 RNAi 2
SA229410191111_FW_1_project_Ermolaeva016ncl-1 RNAi 2
SA229411191111_FW_1_project_Ermolaeva017_TAGIIncl-1 RNAi 6
SA229412191111_FW_1_project_Ermolaeva017ncl-1 RNAi 6
SA229413191111_FW_1_project_Ermolaeva026_TAGIIncl-1 RNAi 6
SA229414191111_FW_1_project_Ermolaeva008ncl-1 RNAi 6
SA229415191111_FW_1_project_Ermolaeva035ncl-1 RNAi 6
SA229416191111_FW_1_project_Ermolaeva035_TAGIIncl-1 RNAi 6
SA229417191111_FW_1_project_Ermolaeva044_TAGIIncl-1 RNAi 6
SA229418191111_FW_1_project_Ermolaeva008_TAGIIncl-1 RNAi 6
SA229419191111_FW_1_project_Ermolaeva044ncl-1 RNAi 6
SA229420191111_FW_1_project_Ermolaeva026ncl-1 RNAi 6
SA229421191111_FW_1_project_Ermolaeva033tif-IA RNAi 12
SA229422191111_FW_1_project_Ermolaeva042_TAGIItif-IA RNAi 12
SA229423191111_FW_1_project_Ermolaeva033_TAGIItif-IA RNAi 12
SA229424191111_FW_1_project_Ermolaeva024_TAGIItif-IA RNAi 12
SA229425191111_FW_1_project_Ermolaeva024tif-IA RNAi 12
SA229426191111_FW_1_project_Ermolaeva006tif-IA RNAi 12
SA229427191111_FW_1_project_Ermolaeva015tif-IA RNAi 12
SA229428191111_FW_1_project_Ermolaeva006_TAGIItif-IA RNAi 12
SA229429191111_FW_1_project_Ermolaeva042tif-IA RNAi 12
SA229430191111_FW_1_project_Ermolaeva015_TAGIItif-IA RNAi 12
SA229431191111_FW_1_project_Ermolaeva004tif-IA RNAi 2
SA229432191111_FW_1_project_Ermolaeva013tif-IA RNAi 2
SA229433191111_FW_1_project_Ermolaeva022tif-IA RNAi 2
SA229434191111_FW_1_project_Ermolaeva040_TAGIItif-IA RNAi 2
SA229435191111_FW_1_project_Ermolaeva013_TAGIItif-IA RNAi 2
SA229436191111_FW_1_project_Ermolaeva022_TAGIItif-IA RNAi 2
SA229437191111_FW_1_project_Ermolaeva004_TAGIItif-IA RNAi 2
SA229438191111_FW_1_project_Ermolaeva040tif-IA RNAi 2
SA229439191111_FW_1_project_Ermolaeva031tif-IA RNAi 2
SA229440191111_FW_1_project_Ermolaeva031_TAGIItif-IA RNAi 2
SA229441191111_FW_1_project_Ermolaeva014_TAGIItif-IA RNAi 6
SA229442191111_FW_1_project_Ermolaeva005_TAGIItif-IA RNAi 6
SA229443191111_FW_1_project_Ermolaeva032tif-IA RNAi 6
SA229444191111_FW_1_project_Ermolaeva023_TAGIItif-IA RNAi 6
SA229445191111_FW_1_project_Ermolaeva041_TAGIItif-IA RNAi 6
SA229446191111_FW_1_project_Ermolaeva005tif-IA RNAi 6
SA229447191111_FW_1_project_Ermolaeva023tif-IA RNAi 6
SA229448191111_FW_1_project_Ermolaeva032_TAGIItif-IA RNAi 6
SA229449191111_FW_1_project_Ermolaeva041tif-IA RNAi 6
SA229450191111_FW_1_project_Ermolaeva014tif-IA RNAi 6
Showing results 1 to 90 of 90

Collection:

Collection ID:CO002406
Collection Summary:Worms were washed and pelleted, M9 media was removed with ~100µl remaining in the sample, and the sample was snap frozen.
Sample Type:Worms

Treatment:

Treatment ID:TR002425
Treatment Summary:Ncl-1 and tif-IA knockdown was achieved by feeding the worms for 2 generations with bacteria (from the Ahringer RNAi feeding library, Source BioScience Ltd.) producing the corresponding dsRNA. Worms were cultured under standard conditions as described in ‘Wormbook’, F1 was grown at 15°C and F2 at 20°C, and F2 animals were grown on RNAi until days 2, 6 or 12 of adulthood.

Sample Preparation:

Sampleprep ID:SP002419
Sampleprep Summary:Triacylglycerols were extracted from C. elegans by successive addition of PBS pH 7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17. Evaporation of the organic layer yielded a lipid film that was dissolved in methanol and subjected to UPLC-MS/MS.
Extract Storage:-20℃

Combined analysis:

Analysis ID AN003795
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity H-Class
Column Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap
Ion Mode POSITIVE
Units relative units

Chromatography:

Chromatography ID:CH002808
Chromatography Summary:Chromatographic separation of triacylglycerols was carried out on an Acquity BEH C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an Acquity UHPLC.
Instrument Name:Waters Acquity H-Class
Column Name:Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS003537
Analysis ID:AN003795
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Targeted MRM with pre-optimized settings and subsequent automated integration of selected signals using Analyst 1.6.2 or Analyst 1.6.3 (Sciex).
Ion Mode:POSITIVE
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