Summary of Study ST002329

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001493. The data can be accessed directly via it's Project DOI: 10.21228/M8R403 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST002329
Study TitleSerum metabonomics of rats in sham, HIE, CO and ASO groups were compared.
Study SummaryTo study the cerebral protective of nervonic acid after hypoxia-ischemia (HI), rats were randomly divided into two groups: Sham group (n=12), HIE group (n=8). After 48 hours of successful modeling, we re-group the HIE group: CO group (n=5) and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil for 30 days, rats in the CO group were treated by an equal volume of corn oil.
Institute
Bao Feng Key Laboratory of Genetics and Metabolism
Last NameChen
First Namexianyang
AddressRoom 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core Zone, Shunyi District, Beijing
Emailcxyibcas@163.com
Phone13681398052
Submit Date2022-10-08
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2026-01-02
Release Version1
xianyang Chen xianyang Chen
https://dx.doi.org/10.21228/M8R403
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001493
Project DOI:doi: 10.21228/M8R403
Project Title:Lipidomic profiling indicate protection of hypoxic-ischemic brain injury on neonatal rats ingesting Acer truncatum Bunge oil
Project Summary:Hypoxic ischemic encephalopathy (HIE) has a great impact on the growth and development of newborns. At present, there is a lack of other effective intervention measures except hypothermia therapy, and the effective rate of hypothermia therapy is less than 50%. Using high-throughput metabonomics, we analyzed the serum and brain tissues of 7-day-old HIE mice, mice fed with Acer truncatum Bunge seed oil (ASO) for 30 days and mice fed with their respective ages. All the results revealed that a variety of metabolites changed significantly during injury and repair, and there were many significant enrichment pathways of designed lipid metabolism and neurotrophic factors. Multi-group comparative analysis identified metabolites that may be related to the injury and repair of hypoxic-ischemic encephalopathy, such as glycerol phospholipids and sphingomyelins. Our results suggest that ASO may be a potential special medical food for ischemic hypoxia newborns.
Institute:Bao Feng Key Laboratory of Genetics and Metabolism
Last Name:Chen
First Name:xianyang
Address:Room 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core Zone, Shunyi District, Beijing
Email:cxyibcas@163.com
Phone:13681398052

Subject:

Subject ID:SU002416
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA22957520210626_91ASO1
SA22957620210624_91ASO1
SA22957720210626_29ASO2
SA22957820210624_29ASO2
SA22957920210624_56ASO3
SA22958020210626_56ASO3
SA22958120210626_13ASO4
SA22958220210624_13ASO4
SA22958320210626_31ASO5
SA22958420210624_31ASO5
SA22958520210624_44ASO6
SA22958620210626_44ASO6
SA22958720210626_52ASO7
SA22958820210624_52ASO7
SA22958920210626_61ASO8
SA22959020210624_61ASO8
SA22959120210624_41CO1
SA22959220210626_41CO1
SA22959320210624_49CO2
SA22959420210626_49CO2
SA22959520210626_36CO3
SA22959620210624_36CO3
SA22959720210624_24CO4
SA22959820210626_24CO4
SA22959920210624_68CO5
SA22960020210626_68CO5
SA22960120210624_12HIE1
SA22960220210626_12HIE1
SA22960320210624_23HIE2
SA22960420210626_23HIE2
SA22960520210626_35HIE3
SA22960620210624_35HIE3
SA22960720210626_47HIE4
SA22960820210624_47HIE4
SA22960920210624_59HIE5
SA22961020210626_59HIE5
SA22961120210624_79HIE6
SA22961220210626_79HIE6
SA22961320210624_63HIE7
SA22961420210626_63HIE7
SA22961520210626_75HIE8
SA22961620210624_75HIE8
SA22961720210626_64sham1
SA22961820210624_64sham1
SA22961920210626_45sham10
SA22962020210624_45sham10
SA22962120210624_43sham11
SA22962220210626_43sham11
SA22962320210626_55sham12
SA22962420210624_55sham12
SA22962520210624_32sham2
SA22962620210626_32sham2
SA22962720210626_18sham3
SA22962820210624_18sham3
SA22962920210626_26sham4
SA22963020210624_26sham4
SA22963120210626_78sham5
SA22963220210624_78sham5
SA22963320210624_80sham6
SA22963420210626_80sham6
SA22963520210624_15sham7
SA22963620210626_15sham7
SA22963720210626_60sham8
SA22963820210624_60sham8
SA22963920210626_28sham9
SA22964020210624_28sham9
Showing results 1 to 66 of 66

Collection:

Collection ID:CO002409
Collection Summary:After sevoflurane inhalation anesthesia, use a blood collection vessel without any additives (without any anticoagulant or accelerator) to collect whole blood. Let it stand at 4 ℃ for about 1h. After the blood coagulates, centrifuge at low speed (3000 rpm, 10 min, 4 ℃), take the upper serum, and centrifuge at high speed (12000 rpm, 10 min, 4 ℃). After centrifugation, take the upper serum and transfer it to a 1.5mL centrifuge tube, 200 μ L Carry out equal subpackaging, make marks, seal with sealing film, and freeze at - 80 ℃.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR002428
Treatment Summary:To study the cerebral protective of nervonic acid after hypoxia-ischemia (HI), rats were randomly divided into two groups: Sham group (n=12), HIE group (n=8). After 48 hours of successful modeling, we re-group the HIE group: CO group (n=5) and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil for 30 days, rats in the CO group were treated by an equal volume of corn oil25. Based on the individual recommended intake NA of 0.3 g/kg/d, the equivalent dose of the drug was calculated according to the bodyweight of the human and the animal, in which the daily dose of the rat was equivalent to six times that of adults.

Sample Preparation:

Sampleprep ID:SP002422
Sampleprep Summary:We use isopropanol (IPA) to extract lipids from serum and brain tissue. In brief, 200 μL of serum was extracted with 600 μL of precooled IPA temperature of 4°C, vortexed for 1 minute and incubated for 10 minutes at room temperature; the extracted mixture was stored overnight at -20 ° C. Samples were centrifuged for 20 minutes at 14000 rpm, and the supernatants were transferred into a new centrifuge tube and diluted to 1:10 with IPA/ACN/H2O (2.5:1:1, v: v: v). Before LC/MS analysis, the sample was stored at -80°C.

Combined analysis:

Analysis ID AN003799 AN003800
Chromatography ID CH002811 CH002811
MS ID MS003541 MS003542
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity I-Class Waters Acquity I-Class
Column Waters Acquity BEH C18 (100 x 2mm,1.7um) Waters Acquity BEH C18 (100 x 2mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Xevo-G2-XS Waters Xevo-G2-XS
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH002811
Chromatography Summary:ACQUITYUPLC I-CLASS (Waters) and XEVO-G2XS quadrupole time-of-flight (QTOF) mass spectrometry (Waters) with ESI were used to analyze the samples.
Methods Filename:Chrom__methods_.pdf
Instrument Name:Waters Acquity I-Class
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Flow Rate:0.4 mL/min
Solvent A:60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS003541
Analysis ID:AN003799
Instrument Name:Waters Xevo-G2-XS
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS003542
Analysis ID:AN003800
Instrument Name:Waters Xevo-G2-XS
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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