Summary of Study ST002476
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001599. The data can be accessed directly via it's Project DOI: 10.21228/M8113P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002476 |
| Study Title | High body temperature increases gut microbiota-dependent host resistance to influenza A virus and SARS-CoV-2 infection (Mouse) |
| Study Summary | While a common symptom of influenza and coronavirus disease 2019 (COVID-19) is fever, its physiological role on host resistance to viral infection remains less clear. Here, we demonstrate that exposure of mice to the high ambient temperature of 36 °C increase host resistance to viral pathogens including influenza virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High heat-exposed mice increase basal body temperature over 38 °C to enable more bile acids production in a gut microbiota-dependent manner. The gut microbiota-derived deoxycholic acid (DCA) and its plasma membrane-bound receptor Takeda G-protein-coupled receptor 5 (TGR5) signaling increase host resistance to influenza virus infection by suppressing virus replication and neutrophil-dependent tissue damage. Furthermore, the DCA and its nuclear farnesoid X receptor (FXR) agonist protect Syrian hamster from lethal SARS-CoV-2 infection. Moreover, we demonstrate that certain bile acids are reduced in the plasma of COVID-19 patients who developed moderate I/II disease compared with minor illness group. These findings uncover an unexpected mechanism by which virus-induced high fever increases host resistance to influenza virus and SARS-CoV-2 in a gut microbiota-dependent manner. |
| Institute | Keio University |
| Last Name | Fukuda |
| First Name | Shinji |
| Address | Kakuganji 246-2, Mizukami, Tsuruoka City Yamagata,Japan |
| sfukuda@sfc.keio.ac.jp | |
| Phone | +81-235-29-0528 |
| Submit Date | 2023-01-24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | CE-MS |
| Release Date | 2023-03-10 |
| Release Version | 2 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001599 |
| Project DOI: | doi: 10.21228/M8113P |
| Project Title: | High body temperature increases gut microbiota-dependent host resistance to influenza A virus and SARS-CoV-2 infection |
| Project Summary: | While a common symptom of influenza and coronavirus disease 2019 (COVID-19) is fever, its physiological role on host resistance to viral infection remains less clear. Here, we demonstrate that exposure of mice to the high ambient temperature of 36 °C increase host resistance to viral pathogens including influenza virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High heat-exposed mice increase basal body temperature over 38 °C to enable more bile acids production in a gut microbiota-dependent manner. The gut microbiota-derived deoxycholic acid (DCA) and its plasma membrane-bound receptor Takeda G-protein-coupled receptor 5 (TGR5) signaling increase host resistance to influenza virus infection by suppressing virus replication and neutrophil-dependent tissue damage. Furthermore, the DCA and its nuclear farnesoid X receptor (FXR) agonist protect Syrian hamster from lethal SARS-CoV-2 infection. Moreover, we demonstrate that certain bile acids are reduced in the plasma of COVID-19 patients who developed moderate I/II disease compared with minor illness group. These findings uncover an unexpected mechanism by which virus-induced high fever increases host resistance to influenza virus and SARS-CoV-2 in a gut microbiota-dependent manner. |
| Institute: | Keio University |
| Last Name: | Fukuda |
| First Name: | Shinji |
| Address: | 246-2 Mizukami, Kakuganji, Tsuruoka-city, Yamagata, 997-0052, Japan |
| Email: | sfukuda@sfc.keio.ac.jp |
| Phone: | +81-235-29-0528 |
Subject:
| Subject ID: | SU002584 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Temp/Duration |
|---|---|---|
| SA249028 | 14 | 22C for 7 d |
| SA249029 | 13 | 22C for 7 d |
| SA249030 | 12 | 22C for 7 d |
| SA249031 | 15 | 22C for 7 d |
| SA249032 | 18 | 22C for 7 d |
| SA249033 | 20 | 22C for 7 d |
| SA249034 | 19 | 22C for 7 d |
| SA249035 | 11 | 22C for 7 d |
| SA249036 | 17 | 22C for 7 d |
| SA249037 | 16 | 22C for 7 d |
| SA249038 | 25 | 36C for 7 d |
| SA249039 | 24 | 36C for 7 d |
| SA249040 | 23 | 36C for 7 d |
| SA249041 | 26 | 36C for 7 d |
| SA249042 | 27 | 36C for 7 d |
| SA249043 | 30 | 36C for 7 d |
| SA249044 | 29 | 36C for 7 d |
| SA249045 | 28 | 36C for 7 d |
| SA249046 | 22 | 36C for 7 d |
| SA249047 | 21 | 36C for 7 d |
| SA249048 | 7 | 4C for 7 d |
| SA249049 | 8 | 4C for 7 d |
| SA249050 | 9 | 4C for 7 d |
| SA249051 | 2 | 4C for 7 d |
| SA249052 | 1 | 4C for 7 d |
| SA249053 | 6 | 4C for 7 d |
| SA249054 | 3 | 4C for 7 d |
| SA249055 | 4 | 4C for 7 d |
| SA249056 | 5 | 4C for 7 d |
| SA249057 | 10 | 4C for 7 d |
| Showing results 1 to 30 of 30 |
Collection:
| Collection ID: | CO002577 |
| Collection Summary: | Cecal contents were obtained by dissection. |
| Sample Type: | cecal contents |
Treatment:
| Treatment ID: | TR002596 |
| Treatment Summary: | NA |
Sample Preparation:
| Sampleprep ID: | SP002590 |
| Sampleprep Summary: | Cecal metabolites were extracted by vigorous shaking with methanol containing methionine sulfone and D-camphol-10-sulfonic acid as the internal standards. |
Combined analysis:
| Analysis ID | AN004077 | AN004078 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent CE | Agilent CE |
| Column | Fused-silica, i.d. 50 µm(cation) | COSMO(+), i.d.50 μm(anion) |
| MS Type | ESI | ESI |
| MS instrument type | TOF | TOF |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | nmol/g | nmol/g |
Chromatography:
| Chromatography ID: | CH003019 |
| Instrument Name: | Agilent CE |
| Column Name: | Fused-silica, i.d. 50 µm(cation) |
| Column Temperature: | none |
| Flow Gradient: | none |
| Flow Rate: | none |
| Solvent A: | none |
| Solvent B: | none |
| Chromatography Type: | CE |
| Chromatography ID: | CH003020 |
| Instrument Name: | Agilent CE |
| Column Name: | COSMO(+), i.d.50 μm(anion) |
| Column Temperature: | none |
| Flow Gradient: | none |
| Flow Rate: | none |
| Solvent A: | none |
| Solvent B: | none |
| Chromatography Type: | CE |
MS:
| MS ID: | MS003824 |
| Analysis ID: | AN004077 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003825 |
| Analysis ID: | AN004078 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | NEGATIVE |
