Summary of Study ST002533

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001629. The data can be accessed directly via it's Project DOI: 10.21228/M84M8C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002533
Study TitleUntargeted Metabolomics on First Trimester Serum Implicates Metabolic Perturbations Associated with BMI in Development of Hypertensive Disorders: A Discovery Study
Study SummaryBody mass index (BMI) in early pregnancy is a critical risk factor for hypertensive disorders of pregnancy (HDP). The pathobiology of the interplay between BMI and HDP is not fully understood and represents the focus of this investigation. BMI and 1st-trimester serum samples were obtained from the Global Alliance to Prevent Prematurity and Stillbirth repository for 154 women (105 without HDP and 49 with HDP). Metabotyping was conducted using ultra-high-performance liquid-chromatography high-resolution mass spectrometry (UHPLC HR-MS). Regression models were used to determine metabolites and pathway perturbations associated with BMI in women with and without HDP, and to determine metabolites and pathway perturbations associated with HDP for women in categories of obese, overweight, and normal weight based on the 1st trimester BMI. This study will lay the groundwork for larger studies of predictive markers and biological pathways leading to infant morbidity and mortality.
Institute
University of North Carolina at Chapel Hill
DepartmentNutrition
Last NameSumner
First NameSusan
Address500 Laureate Way, Kannapolis, NC 28081
Emailsusan_sumner@unc.edu
Phone704-250-5066
Submit Date2023-03-22
Num Groups2
Total Subjects154
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-07-06
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M84M8C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001629
Project DOI:doi: 10.21228/M84M8C
Project Title:Untargeted Metabolomics on First Trimester Serum Implicates Metabolic Perturbations Associated with BMI in Development of Hypertensive Disorders: A Discovery Study
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:Body mass index (BMI) in early pregnancy is a critical risk factor for hypertensive disorders of pregnancy (HDP). The pathobiology of the interplay between BMI and HDP is not fully understood and represents the focus of this investigation. BMI and 1st-trimester serum samples were obtained from the Global Alliance to Prevent Prematurity and Stillbirth repository for 154 women (105 without HDP and 49 with HDP). Metabotyping was conducted using ultra-high-performance liquid-chromatography high-resolution mass spectrometry (UHPLC HR-MS). Regression models were used to determine metabolites and pathway perturbations associated with BMI in women with and without HDP, and to determine metabolites and pathway perturbations associated with HDP for women in categories of obese, overweight, and normal weight based on the 1st trimester BMI. This study will lay the groundwork for larger studies of predictive markers and biological pathways leading to infant morbidity and mortality.
Institute:Tulane University
Department:Department of Epidemiology
Last Name:Harville
First Name:Emily
Address:Department of Epidemiology, Tulane University School of Public Health and Tropical Medicine, 1440 Canal Street, Suite 2001, New Orleans, LA, 70112, USA.
Email:harville@tulane.edu
Phone:504-988-7327
Funding Source:R21

Subject:

Subject ID:SU002633
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Condition
SA254747S_194HDP
SA254748S_193HDP
SA254749S_190HDP
SA254750S_2HDP
SA254751S_37HDP
SA254752S_40HDP
SA254753S_38HDP
SA254754S_188HDP
SA254755S_33HDP
SA254756S_182HDP
SA254757S_16HDP
SA254758S_158HDP
SA254759S_151HDP
SA254760S_160HDP
SA254761S_166HDP
SA254762S_178HDP
SA254763S_175HDP
SA254764S_51HDP
SA254765S_56HDP
SA254766S_85HDP
SA254767S_83HDP
SA254768S_82HDP
SA254769S_89HDP
SA254770S_90HDP
SA254771S_98HDP
SA254772S_95HDP
SA254773S_81HDP
SA254774S_80HDP
SA254775S_62HDP
SA254776S_61HDP
SA254777S_59HDP
SA254778S_68HDP
SA254779S_69HDP
SA254780S_78HDP
SA254781S_75HDP
SA254782S_148HDP
SA254783S_204HDP
SA254784S_103HDP
SA254785S_138HDP
SA254786S_131HDP
SA254787S_14HDP
SA254788S_141HDP
SA254789S_136HDP
SA254790S_104HDP
SA254791S_134HDP
SA254792S_128HDP
SA254793S_122HDP
SA254794S_123HDP
SA254795S_109HDP
SA254796SP_14N/A
SA254797SP_13N/A
SA254798SP_1N/A
SA254799SP_15N/A
SA254800SP_11N/A
SA254801SP_12N/A
SA254802SP_10N/A
SA254803SP_16N/A
SA254804SP_3N/A
SA254805SP_23_3N/A
SA254806SP_5N/A
SA254807SP_6N/A
SA254808SP_9N/A
SA254809SP_8N/A
SA254810SP_7N/A
SA254811SP_23_2N/A
SA254812SP_4N/A
SA254813SP_23N/A
SA254814SP_2N/A
SA254815SP_18N/A
SA254816SP_17N/A
SA254817SP_21N/A
SA254818SP_20N/A
SA254819SP_19N/A
SA254820S_53Without HDP
SA254821S_57Without HDP
SA254822S_121Without HDP
SA254823S_58Without HDP
SA254824S_126Without HDP
SA254825S_124Without HDP
SA254826S_54Without HDP
SA254827S_48Without HDP
SA254828S_42Without HDP
SA254829S_41Without HDP
SA254830S_127Without HDP
SA254831S_43Without HDP
SA254832S_120Without HDP
SA254833S_47Without HDP
SA254834S_44Without HDP
SA254835S_50Without HDP
SA254836S_106Without HDP
SA254837S_91Without HDP
SA254838S_130Without HDP
SA254839S_107Without HDP
SA254840S_87Without HDP
SA254841S_92Without HDP
SA254842S_94Without HDP
SA254843S_102Without HDP
SA254844S_99Without HDP
SA254845S_96Without HDP
SA254846S_86Without HDP
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Collection:

Collection ID:CO002626
Collection Summary:N/A
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002645
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002639
Sampleprep Summary:Samples were stored at -80 °C in the Global Alliance to Prevent Prematurity and Stillbirth (GAPPS) repository before being shipped (on dry ice) to Nutrition Research Institute, UNC-CH, for metabolomics analysis. Serum samples were thawed at 4 °C overnight. Samples were vortexed via a multiple-tube vortex mixer for 2 min at 5000 rpm and then sat on ice for 10 min before sample preparation. Quality control samples (QC pools) were made by pooling 7-µL serum from each parent sample (if sufficient volume is available). An aliquot of the sample (50-µL), including the study sample and QC pool, was transferred into a new pre-labeled tube and mixed with 400-µL methanol containing 500 ng/ml L-tryptophan-d5, vortexed at 5,000 rpm for 2 min, for protein precipitation. All samples were centrifuged at 16, 000 rcf for 10 min at 4°C. The supernatant (350-uL) was dried by a SpeedVac overnight. The dried extracts were reconstituted with 100 µl water-methanol (95:5, v/v) by thoroughly mixing on multiple tube vortex mixer for 10 min at 5000 rpm and centrifuging at 4°C for 10 min at 16,000 rcf. The supernatant (about 100 µL) was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:On ice
Extraction Method:Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard
Extract Storage:-80℃
Sample Resuspension:Water-Methanol (95:5, v/v)
Sample Spiking:Tryptophan-d5 stock solution at 500 ng/mL

Combined analysis:

Analysis ID AN004168
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH003086
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:50
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5
Flow Rate:0.4 mL/min
Injection Temperature:8
Internal Standard:Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22
Weak Wash Solvent Name:10% methanol/90% water; 0.1% formic acid
Strong Wash Solvent Name:75% 2-Propanol/25% Water; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS003915
Analysis ID:AN004168
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:275 C
Collision Energy:20-45, ramp
Fragmentation Method:CID
Ion Spray Voltage:3.5 kV
Ionization:ES+
Mass Accuracy:5 ppm
Dataformat:Profile
Desolvation Gas Flow:45
Desolvation Temperature:325 C
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