Summary of Study ST002728

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001692. The data can be accessed directly via it's Project DOI: 10.21228/M80H7Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002728
Study TitleThe ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (CHES Cohort)
Study TypeProspective Cohort Study
Study SummaryThe aims of the NYU Children’s Health and Environment Study (CHES) are to evaluate influences of prenatal non-persistent chemical exposures on fetal and postnatal growth and pool our data with the US National Institutes of Health Environmental influences on Child Health Outcomes (ECHO) Program to answer collaborative research questions on the impact of the preconceptual, prenatal, and postnatal environment on childhood obesity, neurodevelopment, pre/peri/postnatal outcomes, upper and lower airway outcomes, and positive health. Eligible women were ≥ 18 years old, < 18 weeks pregnant, had a pregnancy that is not medically threatened, and planned to deliver at NYU Langone Hospital—Manhattan, Bellevue Hospital, or NYU Langone Hospital—Brooklyn. Data collection in pregnancy was nested in clinical care, with questionnaire and specimen collection conducted during routine prenatal visits at < 18, 18–25, and > 25 weeks gestation. These have been followed by questionnaire and specimen collection at birth and regular postpartum intervals. Please contact Dr. Leonardo Trasande at Leonardo.Trasande@nyulangone.org for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) Program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. CHES is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
NYU Grossman School of Medicine
DepartmentDepartment of Pediatrics
LaboratoryDivision of Environmental Pediatrics
Last NameTrasande
First NameLeonardo
AddressNYU Langone Health 227 E 30th St, 8th Floor New York, NY 10016
EmailLeonardo.Trasande@nyulangone.org
Phone(646) 501-2520
Submit Date2023-05-18
Total Subjects500
Study CommentsHHEAR Project EM20-0011, ECHO Project EC0376
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Chear StudyYes
Analysis Type DetailLC-MS
Release Date2024-05-18
Release Version1
Leonardo Trasande Leonardo Trasande
https://dx.doi.org/10.21228/M80H7Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001692
Project DOI:doi: 10.21228/M80H7Q
Project Title:The ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (CHES Cohort)
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:This project was funded by the NIH Environmental influences on Child Health Outcomes (ECHO) Program. Untargeted metabolomics profiling data was acquired from urine samples provided by 14 ECHO cohorts.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Rushing
First Name:Blake
Address:Nutrition Research Institute , University of North Carolina at Chapel Hill, 500 Laureate Way Kannapolis, NC 28081
Email:blake_rushing@unc.edu
Phone:(704) 282-9838
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) Program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The CHES ECHO Cohort is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539

Subject:

Subject ID:SU002834
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type Batch
SA274027CHES_B_8Blank 1
SA274028CHES_B_1Blank 1
SA274029CHES_B_7Blank 1
SA274030CHES_B_6Blank 1
SA274031CHES_B_2Blank 1
SA274032CHES_B_4Blank 1
SA274033CHES_B_3Blank 1
SA274034CHES_B_5Blank 1
SA274035CHES_B_15Blank 2
SA274036CHES_B_16Blank 2
SA274037CHES_B_14Blank 2
SA274038CHES_B_10Blank 2
SA274039CHES_B_9Blank 2
SA274040CHES_B_13Blank 2
SA274041CHES_B_11Blank 2
SA274042CHES_B_12Blank 2
SA274043CHES_B_23Blank 3
SA274044CHES_B_22Blank 3
SA274045CHES_B_24Blank 3
SA274046CHES_B_18Blank 3
SA274047CHES_B_21Blank 3
SA274048CHES_B_19Blank 3
SA274049CHES_B_17Blank 3
SA274050CHES_B_20Blank 3
SA274051CHES_B_30Blank 4
SA274052CHES_B_32Blank 4
SA274053CHES_B_31Blank 4
SA274054CHES_B_29Blank 4
SA274055CHES_B_25Blank 4
SA274056CHES_B_28Blank 4
SA274057CHES_B_26Blank 4
SA274058CHES_B_27Blank 4
SA274059CHES_B_38Blank 5
SA274060CHES_B_37Blank 5
SA274061CHES_B_40Blank 5
SA274062CHES_B_39Blank 5
SA274063CHES_B_36Blank 5
SA274064CHES_B_35Blank 5
SA274065CHES_B_34Blank 5
SA274066CHES_B_33Blank 5
SA274067CHES_B_48Blank 6
SA274068CHES_B_49Blank 6
SA274069CHES_B_50Blank 6
SA274070CHES_B_46Blank 6
SA274071CHES_B_47Blank 6
SA274072CHES_B_45Blank 6
SA274073CHES_B_41Blank 6
SA274074CHES_B_42Blank 6
SA274075CHES_B_43Blank 6
SA274076CHES_B_44Blank 6
SA274077CHES_CHEAR_U_4CHEAR 1
SA274078CHES_CHEAR_U_1CHEAR 1
SA274079CHES_CHEAR_U_3CHEAR 1
SA274080CHES_CHEAR_U_2CHEAR 1
SA274081CHES_CHEAR_U_8CHEAR 2
SA274082CHES_CHEAR_U_7CHEAR 2
SA274083CHES_CHEAR_U_5CHEAR 2
SA274084CHES_CHEAR_U_6CHEAR 2
SA274085CHES_CHEAR_U_12CHEAR 3
SA274086CHES_CHEAR_U_11CHEAR 3
SA274087CHES_CHEAR_U_9CHEAR 3
SA274088CHES_CHEAR_U_10CHEAR 3
SA274089CHES_CHEAR_U_16CHEAR 4
SA274090CHES_CHEAR_U_13CHEAR 4
SA274091CHES_CHEAR_U_15CHEAR 4
SA274092CHES_CHEAR_U_14CHEAR 4
SA274093CHES_CHEAR_U_20CHEAR 5
SA274094CHES_CHEAR_U_17CHEAR 5
SA274095CHES_CHEAR_U_19CHEAR 5
SA274096CHES_CHEAR_U_18CHEAR 5
SA274097CHES_CHEAR_U_24CHEAR 6
SA274098CHES_CHEAR_U_25CHEAR 6
SA274099CHES_CHEAR_U_23CHEAR 6
SA274100CHES_CHEAR_U_22CHEAR 6
SA274101CHES_CHEAR_U_21CHEAR 6
SA274102CHES_HHEAR_U1_4HHEAR U1 1
SA274103CHES_HHEAR_U1_3HHEAR U1 1
SA274104CHES_HHEAR_U1_2HHEAR U1 1
SA274105CHES_HHEAR_U1_1HHEAR U1 1
SA274106CHES_HHEAR_U1_8HHEAR U1 2
SA274107CHES_HHEAR_U1_7HHEAR U1 2
SA274108CHES_HHEAR_U1_5HHEAR U1 2
SA274109CHES_HHEAR_U1_6HHEAR U1 2
SA274110CHES_HHEAR_U1_12HHEAR U1 3
SA274111CHES_HHEAR_U1_9HHEAR U1 3
SA274112CHES_HHEAR_U1_11HHEAR U1 3
SA274113CHES_HHEAR_U1_10HHEAR U1 3
SA274114CHES_HHEAR_U1_16HHEAR U1 4
SA274115CHES_HHEAR_U1_13HHEAR U1 4
SA274116CHES_HHEAR_U1_15HHEAR U1 4
SA274117CHES_HHEAR_U1_14HHEAR U1 4
SA274118CHES_HHEAR_U1_20HHEAR U1 5
SA274119CHES_HHEAR_U1_19HHEAR U1 5
SA274120CHES_HHEAR_U1_18HHEAR U1 5
SA274121CHES_HHEAR_U1_17HHEAR U1 5
SA274122CHES_HHEAR_U1_25HHEAR U1 6
SA274123CHES_HHEAR_U1_24HHEAR U1 6
SA274124CHES_HHEAR_U1_23HHEAR U1 6
SA274125CHES_HHEAR_U1_22HHEAR U1 6
SA274126CHES_HHEAR_U1_21HHEAR U1 6
Showing page 1 of 7     Results:    1  2  3  4  5  Next  Last     Showing results 1 to 100 of 693

Collection:

Collection ID:CO002827
Collection Summary:N/A
Sample Type:Urine
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002843
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002840
Sampleprep Summary:Study samples were shipped from the NYU Grossman School of Medicine to the NC HHEAR Hub on dry ice. The NC HHEAR Hub thawed and transferred 50 µL of the study samples to a new set of tubes and used them for the analysis. An additional 10 µL was taken from the original study sample and transferred to another tube to make the total study pool for this project, and then distributed with 50 µL per aliquot, used as quality control study pools (QC study pools) throughout the whole analysis. All sample aliquots (50 µL each) and QC study pools (50 µL each) were stored at -80° C until the day of sample preparation. HHEAR Urine Pool 1 (50 µL each), HHEAR Urine Pool 2 (50 µL each), CHEAR reference urine (50 µL each) and NIST urine (SRM 3672) reference material (50 µL each) were provided by the NC HHEAR Hub. LC-MS grade water (50 µL) was used as blanks. All samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference urine, CHEAR reference urine, NIST reference urine, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:4℃
Extraction Method:Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard
Extract Storage:-80℃
Sample Resuspension:Water-Methanol (95:5, v/v)
Sample Spiking:Tryptophan-d5 stock solution at 500 ng/mL

Combined analysis:

Analysis ID AN004422
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units Peak Area

Chromatography:

Chromatography ID:CH003321
Chromatography Summary:Reversed phase
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6000-10000 psi
Column Temperature:50 ℃
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 mL/min
Injection Temperature:8 ℃
Internal Standard:Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Weak Wash Solvent Name:10% methanol/90% water; 0.1% formic acid
Strong Wash Solvent Name:75% 2-Propanol/25% Water; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004169
Analysis ID:AN004422
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 KV
Collision Energy:20-45, ramp
Collision Gas:N2
Fragmentation Method:CID
Ion Spray Voltage:3.5kV
Ionization:ES+
Mass Accuracy:5 ppm
Dataformat:Profile
Desolvation Gas Flow:55
Desolvation Temperature:400 ℃
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