Summary of Study ST002826

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001768. The data can be accessed directly via it's Project DOI: 10.21228/M85T5M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002826
Study TitleCold-stimulated brown adipose tissue activation is related to changes in serum metabolites relevant to NAD+ metabolism in humans
Study SummaryCold-induced brown adipose tissue (BAT) activation is considered to improve metabolic health. In murine BAT, cold increases the fundamental molecule for mitochondrial function, nicotinamide adenine dinucleotide (NAD+), but limited knowledge on NAD+ metabolism during cold in human BAT metabolism exists. We show that cold increases the serum metabolites of the NAD+ salvage pathway (nicotinamide and 1-methylnicotinamide) in humans. Additionally, individuals with coldstimulated BAT activation have decreased levels of metabolites from the de novo NAD+ biosynthesis pathway (tryptophan, kynurenine). Serum nicotinamide correlates positively with cold-stimulated BAT activation, whereas tryptophan and kynurenine correlates negatively. Furthermore, the expression of genes involved in NAD+ biosynthesis in BAT are related to markers of metabolic health. Our data indicate that cold increases serum tryptophan conversion to nicotinamide to be further utilized by BAT. We conclude that NAD+ metabolism is activated upon cold in humans and probably regulated in a coordinated fashion by several tissues.
Institute
University of Turku
Last NameVirtanen
First NameKirsi
AddressTurku PET Centre, Turku University Hospital, Turku, Finland
Emailkirsi.virtanen@utu.fi
Phone+358-40-7626564
Submit Date2023-08-10
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2023-09-11
Release Version1
Kirsi Virtanen Kirsi Virtanen
https://dx.doi.org/10.21228/M85T5M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001768
Project DOI:doi: 10.21228/M85T5M
Project Title:Cold-stimulated brown adipose tissue activation is related to changes in serum metabolites relevant to NAD+ metabolism in humans
Project Summary:Cold-induced brown adipose tissue (BAT) activation is considered to improve metabolic health. In murine BAT, cold increases the fundamental molecule for mitochondrial function, nicotinamide adenine dinucleotide (NAD+), but limited knowledge on NAD+ metabolism during cold in human BAT metabolism exists. We show that cold increases the serum metabolites of the NAD+ salvage pathway (nicotinamide and 1-methylnicotinamide) in humans. Additionally, individuals with coldstimulated BAT activation have decreased levels of metabolites from the de novo NAD+ biosynthesis pathway (tryptophan, kynurenine). Serum nicotinamide correlates positively with cold-stimulated BAT activation, whereas tryptophan and kynurenine correlates negatively. Furthermore, the expression of genes involved in NAD+ biosynthesis in BAT are related to markers of metabolic health. Our data indicate that cold increases serum tryptophan conversion to nicotinamide to be further utilized by BAT. We conclude that NAD+ metabolism is activated upon cold in humans and probably regulated in a coordinated fashion by several tissues.
Institute:University of Turku
Last Name:Virtanen
First Name:Kirsi A
Address:Turku PET Centre, Turku University Hospital, Turku, Finland
Email:kirsi.virtanen@utu.fi
Phone:+358-040-7626564

Subject:

Subject ID:SU002935
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA30575063cold
SA30575167cold
SA30575262cold
SA30575369cold
SA30575460cold
SA30575555cold
SA30575670cold
SA30575757cold
SA30575858cold
SA30575942cold
SA30576061cold
SA30576184cold
SA30576294cold
SA30576393cold
SA30576495cold
SA30576596cold
SA30576699cold
SA30576798cold
SA30576891cold
SA30576989cold
SA30577076cold
SA30577175cold
SA30577280cold
SA30577381cold
SA30577486cold
SA30577541cold
SA30577673cold
SA30577751cold
SA30577820cold
SA30577919cold
SA30578018cold
SA30578116cold
SA30578221cold
SA30578322cold
SA30578427cold
SA30578540cold
SA30578623cold
SA30578715cold
SA30578814cold
SA3057897cold
SA3057906cold
SA3057913cold
SA3057921cold
SA3057938cold
SA3057949cold
SA30579513cold
SA30579612cold
SA30579711cold
SA30579828cold
SA30579924cold
SA30580038cold
SA30580131cold
SA30580233cold
SA30580334cold
SA30580436cold
SA30580537cold
SA30580630cold
SA30580732cold
SA30580829cold
SA30580939cold
SA305734QC3QC
SA305735QC6QC
SA305736QC1QC
SA305737QC9QC
SA305738QC4QC
SA305739QC8QC
SA305740QC7QC
SA305741QC10QC
SA305742QC5QC
SA305743QC2QC
SA305744QC autoMSMS 40V_1QC
SA305745QC autoMSMS 40V_2QC
SA305746QC autoMSMS 20V_1QC
SA305747QC autoMSMS 10V_1QC
SA305748QC autoMSMS 20V_2QC
SA305749QC autoMSMS 10V_2QC
SA30581043warm
SA30581150warm
SA30581253warm
SA30581392warm
SA3058144warm
SA30581552warm
SA30581649warm
SA30581746warm
SA305818102warm
SA30581954warm
SA305820100warm
SA305821101warm
SA30582245warm
SA30582397warm
SA30582417warm
SA30582571warm
SA30582672warm
SA30582725warm
SA30582826warm
SA30582968warm
SA30583066warm
SA30583164warm
SA30583277warm
SA30583378warm
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Collection:

Collection ID:CO002928
Collection Summary:Blood samples were either taken during the exposure to room temperature or during cold (subsequent to 2 hours of personalised cooling). More details are in the manuscript.
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002944
Treatment Summary:The exposure to cold for 2 hours under cooling blankets using a personalised cooling protocol

Sample Preparation:

Sampleprep ID:SP002941
Sampleprep Summary:Fasting serum samples taken during the cold or RT exposures were stored at -80 °C and thawed in an ice water bath. Four hundred microliters of ice-cold acetonitrile (ACN) was pipetted to 96-well filter plates (Captiva ND Plate, 0.2 μm PP, Agilent Technologies, Santa Clara, CA, United States) after which 100 μL of plasma or quality control (QC) was added. The solution was mixed by pipetting up and down, filtrated by centrifugation (700 RCF, 4 °C, 5 min) and collected to a 96-1 well plate (96 deep well plate natural,Thermo Scientific). QC was serum sample prepared by pooling an aliquot from all analysed serum samples.

Combined analysis:

Analysis ID AN004610 AN004611 AN004612 AN004613
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Agilent 1290 Agilent 1290 Agilent 1290 Agilent 1290
Column Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm
MS Type ESI ESI ESI ESI
MS instrument type QTOF QTOF QTOF QTOF
MS instrument name Agilent 6540 QTOF Agilent 6540 QTOF Agilent 6540 QTOF Agilent 6540 QTOF
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Area Area Area Area

Chromatography:

Chromatography ID:CH003468
Instrument Name:Agilent 1290
Column Name:Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm
Column Temperature:45
Flow Gradient:0–2.5 min: 100% B, 2.5–10 min: 100% B → 0% B; 10–10.01 min: 0% B → 100% B; 10.01–12.5 min: 100% B.
Flow Rate:0.6 mL/min.
Solvent A:50% acetonitrile with 20 mM ammonium formate and 0.25% formic acid, pH 3
Solvent B:90% acetonitrile with 20 mM ammonium formate, 0.25% formic acid, pH 3.
Chromatography Type:HILIC
  
Chromatography ID:CH003469
Instrument Name:Agilent 1290
Column Name:Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm
Column Temperature:50
Flow Gradient:2 to 100% B in 10 min; 100% B from 10 min to 14.5 min; 100% to 2% B, from 14.51 min to 16.5 min
Flow Rate:0.4 mL/min.
Solvent A:water with 0.1% (v/v) of formic acid
Solvent B:methanol with 0.1% (v/v) of formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004356
Analysis ID:AN004610
Instrument Name:Agilent 6540 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The data acquisition software was the MassHunter Acquisition B.05.01 (Agilent Technologies).
Ion Mode:POSITIVE
  
MS ID:MS004357
Analysis ID:AN004611
Instrument Name:Agilent 6540 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The data acquisition software was the MassHunter Acquisition B.05.01 (Agilent Technologies).
Ion Mode:NEGATIVE
  
MS ID:MS004358
Analysis ID:AN004612
Instrument Name:Agilent 6540 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The data acquisition software was the MassHunter Acquisition B.05.01 (Agilent Technologies).
Ion Mode:POSITIVE
  
MS ID:MS004359
Analysis ID:AN004613
Instrument Name:Agilent 6540 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The data acquisition software was the MassHunter Acquisition B.05.01 (Agilent Technologies).
Ion Mode:NEGATIVE
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