Summary of Study ST003002

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001868. The data can be accessed directly via it's Project DOI: 10.21228/M8842H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003002
Study TitleThe role of gut microbiota in muscle mitochondria function, colon health, and sarcopenia: from clinical to bench (2)
Study SummaryGut microbes produce metabolites in the intestinal lumen, which can travel systemically to affect organs. Metagenomic analysis was performed on liquid chromatography–mass spectrometry (LC-MS) to detect differential metabolites between people with or without sarcopenia.
Institute
The Chinese University of Hong Kong
Last NameWong
First NamePui Yan
Address30-32 Ngan Shing Street , Shatin, New Territories
Emailpuiyanwong@cuhk.edu.hk
Phone(852)-35052756
Submit Date2023-12-10
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailGC/LC-MS
Release Date2024-12-30
Release Version1
Pui Yan Wong Pui Yan Wong
https://dx.doi.org/10.21228/M8842H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001868
Project DOI:doi: 10.21228/M8842H
Project Title:From clinical to benchside: Lacticaseibacillus and Faecalibacterium are positively associated with muscle health and alleviate age-related muscle disorder
Project Summary:Sarcopenia is an age-related muscle disorder that increases the risk of adverse clinical outcomes, but its pathogenesis is unclear and treatments are limited. Increasing evidence shows that gut microbiota is potentially associated with sarcopenia. To investigate its role in sarcopenia, we (i) compared gut microbiota and metabolite composition between older persons with and without sarcopenia, (ii) performed fecal microbiota transplantation (FMT) from human donors to antibiotic-treated mice, and (iii) identified two specific probiotics for treatment of sarcopenia in aged mice. Metagenomic and metabolomic analyses showed that people with sarcopenia had different microbial composition and metabolites, and that fecal purine could accurately identify sarcopenia. After FMT, mice receiving microbes from people with sarcopenia displayed lower muscle mass and strength. Correlation analysis revealed Lacticaseibacillus rhamnosus (LR) and Faecalibacterium prausnitzii (FP) were positively related to muscle health in old people. Both probiotic LR, FP and their combination enhanced muscle mass, function, and fiber type proportion of aged mice. Transcriptomics showed that genes related to tricarboxylic acid cycle were enriched after treatment. Mitochondria density, muscle ATP content, NAD+/NADH, proteins related to mitochondrial dynamics and biogenesis were improved by both probiotics. In in-vitro studies, probiotic-conditional medium (PCM) containing FP supernatant or the combination of FP and LR supernatants enhanced proliferation of C2C12 myoblasts, whilst LR PCM alone did not. The mechanisms of LR may be related to colon health improvement. Results showed gut microbiota dysbiosis is one of pathogenic factors of sarcopenia, and muscle-related probiotics could alleviate age-related muscle disorders. Further clinical translation is warranted.
Institute:The Chinese University of Hong Kong
Department:ORT
Laboratory:The Prince of Wales Hospital
Last Name:Wong
First Name:Pui Yan
Address:30-32 Ngan Shing Street , Shatin, New Territories
Email:puiyanwong@cuhk.edu.hk
Phone:(852)-35052756

Subject:

Subject ID:SU003115
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Status
SA326698NS_17non-sarcopenia
SA326699NS_16non-sarcopenia
SA326700NS_15non-sarcopenia
SA326701NS_18non-sarcopenia
SA326702NS_14non-sarcopenia
SA326703NS_21non-sarcopenia
SA326704NS_1non-sarcopenia
SA326705NS_23non-sarcopenia
SA326706NS_22non-sarcopenia
SA326707NS_13non-sarcopenia
SA326708NS_20non-sarcopenia
SA326709NS_19non-sarcopenia
SA326710NS_4non-sarcopenia
SA326711NS_6non-sarcopenia
SA326712NS_3non-sarcopenia
SA326713NS_12non-sarcopenia
SA326714NS_2non-sarcopenia
SA326715NS_7non-sarcopenia
SA326716NS_5non-sarcopenia
SA326717NS_11non-sarcopenia
SA326718NS_9non-sarcopenia
SA326719NS_10non-sarcopenia
SA326720NS_8non-sarcopenia
SA326721S_20sarcopenia
SA326722S_21sarcopenia
SA326723S_19sarcopenia
SA326724S_18sarcopenia
SA326725S_17sarcopenia
SA326726S_22sarcopenia
SA326727S_28sarcopenia
SA326728S_27sarcopenia
SA326729S_16sarcopenia
SA326730S_26sarcopenia
SA326731S_25sarcopenia
SA326732S_24sarcopenia
SA326733S_23sarcopenia
SA326734S_3sarcopenia
SA326735S_6sarcopenia
SA326736S_7sarcopenia
SA326737S_5sarcopenia
SA326738S_4sarcopenia
SA326739S_1sarcopenia
SA326740S_2sarcopenia
SA326741S_8sarcopenia
SA326742S_9sarcopenia
SA326743S_13sarcopenia
SA326744S_14sarcopenia
SA326745S_12sarcopenia
SA326746S_11sarcopenia
SA326747S_10sarcopenia
SA326748S_15sarcopenia
Showing results 1 to 51 of 51

Collection:

Collection ID:CO003108
Collection Summary:Participants were asked to bring home a bag of collection tools and instruction flow chart. Briefly, the water ice pack was stored in -20 ℃ home freezer before stool was available. A clean plastic bottle and an oblate stick were used to collect fresh stool samples. Participants put the ice pack and the bottle into a thermal bag, and brought back to the hospital within 2 hours. Fresh stool samples were separated into small sterile tubes, and frozen by liquid nitrogen immediately. Tubes were stored at -80 ℃ until use.
Sample Type:Feces

Treatment:

Treatment ID:TR003124
Treatment Summary:For LC-MS analysis of human stool samples, 20 mg stool samples from each participant (n = 51) were added with 400 μL solution (methanol: water = 7:3 V/V) with internal standard, and vortexed for 3 min. After sonication in an ice bath for 10 min and vortex for 1 min, the sample was placed at -20 °C for 30 minutes. Subsequently, the sample was centrifugated at 12,000 rpm for 10 min at 4 °C, after which the sediment was discarded, and the supernatant was centrifuged again at 12,000 rpm for 3 min at 4°C. 200 μL of the supernatant was collected for analysis.

Sample Preparation:

Sampleprep ID:SP003121
Sampleprep Summary:For LC-MS analysis of human stool samples, 20 mg stool samples from each participant (n = 51) were added with 400 μL solution (methanol: water = 7:3 V/V) with internal standard, and vortexed for 3 min. After sonication in an ice bath for 10 min and vortex for 1 min, the sample was placed at -20 °C for 30 minutes. Subsequently, the sample was centrifugated at 12,000 rpm for 10 min at 4 °C, after which the sediment was discarded, and the supernatant was centrifuged again at 12,000 rpm for 3 min at 4°C. 200 μL of the supernatant was collected for analysis.

Combined analysis:

Analysis ID AN004930 AN004931
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6545 QTOF 1290 Infinity LC UPLC
Ion Mode NEGATIVE POSITIVE
Units nmol/g nmol/g

Chromatography:

Chromatography ID:CH003721
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:40 ℃
Flow Gradient:A:B=95:5 V/V at 0 min, followed by 10:90 V/V at 11.0 min, 10:90 V/V at 12.0 min, 95:5 V/V at 12.1 min and 95:5 V/V at 14.0 min.
Flow Rate:0.4 mL/min
Solvent A:0.1% formic acid in water
Solvent B:0.1% formic acid in acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS004673
Analysis ID:AN004930
Instrument Name:Agilent 6545 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:LC-MS/MS analyses were performed using an UHPLC system (Vanquish, Thermo Fisher Scientific) with a UPLC BEH Amide column (2.1 mm × 100 mm, 1.7 μm) coupled to Orbitrap Exploris 120 mass spectrometer (Orbitrap MS, Thermo). The mobile phase consisted of 25 mmol/L ammonium acetate and 25 ammonia hydroxide in water pH = 9.75））(A) and acetonitrile (B). The auto-sampler temperature was 4 ℃℃, and the injection volume was 2 μμL. The Orbitrap Exploris 120 mass spectrometer was used for its ability to acquire MS/MS spectra on information-dependent acquisition (IDA) mode in the control of the acquisition software (Xcalibur, Thermo). In this mode, the acquisition software continuously evaluates the full scan MS spectrum. The ESI source conditions were set as following: sheath gas flow rate as 50 Arb, Aux gas flow rate as 15 Arb, capillary temperature 320 ℃℃, full MS resolution as 60000, MS/MS resolution as 15000 collision energy as 10/30/60 in NCE mode, spray Voltage as 3.8 kV (positive) or -3.4 kV (negative), respectively.
Ion Mode:NEGATIVE
  
MS ID:MS004674
Analysis ID:AN004931
Instrument Name:1290 Infinity LC UPLC
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:LC-MS/MS analyses were performed using an UHPLC system (Vanquish, Thermo Fisher Scientific) with a UPLC BEH Amide column (2.1 mm × 100 mm, 1.7 μm) coupled to Orbitrap Exploris 120 mass spectrometer (Orbitrap MS, Thermo). The mobile phase consisted of 25 mmol/L ammonium acetate and 25 ammonia hydroxide in water pH = 9.75））(A) and acetonitrile (B). The auto-sampler temperature was 4 ℃℃, and the injection volume was 2 μμL. The Orbitrap Exploris 120 mass spectrometer was used for its ability to acquire MS/MS spectra on information-dependent acquisition (IDA) mode in the control of the acquisition software (Xcalibur, Thermo). In this mode, the acquisition software continuously evaluates the full scan MS spectrum. The ESI source conditions were set as following: sheath gas flow rate as 50 Arb, Aux gas flow rate as 15 Arb, capillary temperature 320 ℃℃, full MS resolution as 60000, MS/MS resolution as 15000 collision energy as 10/30/60 in NCE mode, spray Voltage as 3.8 kV (positive) or -3.4 kV (negative), respectively.
Ion Mode:POSITIVE
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