Summary of Study ST003136

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001949. The data can be accessed directly via it's Project DOI: 10.21228/M8SM7W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003136
Study TitleCirculating N-lactoyl-amino acids and N-formyl-methionine reflect mitochondrial dysfunction and predict mortality in septic shock
Study Summaryhe aim of this study was to identify circulating markers that correlated with severity and mortality in sepsis. We performed metabolomic profiling on 4 cohorts: 1) patients with septic shock, 2) patients with bacteremia without sepsis, 3) cardiogenic shock, and 4) healthy ambulatory controls. We found a set of metabolites that out outperformed lactate in their ability to distinguish survivors and non-survivors of sepsis.
Institute
Massachusetts General Hospital
Last NameSharma
First NameRohit
Address185 Cambridge Street, 6th Floor, Boston, MA, 02114, USA
Emailrsharma2@partners.org
Phone(617) 643-3059
Submit Date2024-01-03
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2025-01-05
Release Version1
Rohit Sharma Rohit Sharma
https://dx.doi.org/10.21228/M8SM7W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001949
Project DOI:doi: 10.21228/M8SM7W
Project Title:Circulating N-lactoyl-amino acids and N-formyl-methionine reflect mitochondrial dysfunction and predict mortality in septic shock
Project Summary:The aim of this study was to identify circulating markers that correlated with severity and mortality in sepsis. We performed metabolomic profiling on 4 cohorts: 1) patients with septic shock, 2) patients with bacteremia without sepsis, 3) cardiogenic shock, and 4) healthy ambulatory controls. We found a set of metabolites that out outperformed lactate in their ability to distinguish survivors and non-survivors of sepsis.
Institute:Massachusetts General Hospital
Department:Molecular Biology
Laboratory:Mootha Laboratory
Last Name:Sharma
First Name:Rohit
Address:185 Cambridge Street, 6th Floor, Boston, MA, 02114, USA
Email:rsharma2@partners.org
Phone:(617) 643-3059

Subject:

Subject ID:SU003253
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Study_group
SA339490RR_076Ambulatory Control
SA339491RR_077Ambulatory Control
SA339492RR_079Ambulatory Control
SA339493RR_073Ambulatory Control
SA339494RR_078Ambulatory Control
SA339495RR_071Ambulatory Control
SA339496RR_063Ambulatory Control
SA339497RR_066Ambulatory Control
SA339498RR_067Ambulatory Control
SA339499RR_080Ambulatory Control
SA339500RR_072Ambulatory Control
SA339501RR_075Ambulatory Control
SA339502RR_081Ambulatory Control
SA339503RR_092Ambulatory Control
SA339504RR_093Ambulatory Control
SA339505RR_094Ambulatory Control
SA339506RR_089Ambulatory Control
SA339507RR_082Ambulatory Control
SA339508RR_083Ambulatory Control
SA339509RR_054Bacteremia without Sepsis
SA339510RR_052Bacteremia without Sepsis
SA339511RR_053Bacteremia without Sepsis
SA339512RR_051Bacteremia without Sepsis
SA339513RR_047Bacteremia without Sepsis
SA339514RR_049Bacteremia without Sepsis
SA339515RR_050Bacteremia without Sepsis
SA339516RR_057Bacteremia without Sepsis
SA339517RR_104Bacteremia without Sepsis
SA339518RR_090Bacteremia without Sepsis
SA339519RR_043Bacteremia without Sepsis
SA339520RR_084Bacteremia without Sepsis
SA339521RR_091Bacteremia without Sepsis
SA339522RR_062Bacteremia without Sepsis
SA339523RR_060Bacteremia without Sepsis
SA339524RR_061Bacteremia without Sepsis
SA339525RR_058Bacteremia without Sepsis
SA339526RR_056Bacteremia without Sepsis
SA339527RR_022Cardiogeneic Shock
SA339528RR_020Cardiogeneic Shock
SA339529RR_025Cardiogeneic Shock
SA339530RR_030Cardiogeneic Shock
SA339531RR_059Cardiogeneic Shock
SA339532RR_041Cardiogeneic Shock
SA339533RR_015Cardiogeneic Shock
SA339534RR_010Cardiogeneic Shock
SA339535RR_005Cardiogeneic Shock
SA339536RR_085Cardiogeneic Shock
SA339537RR_011Cardiogeneic Shock
SA339538RR_013Cardiogeneic Shock
SA339539RR_031Cardiogeneic Shock
SA339540RR_019Cardiogeneic Shock
SA339541RR_055Cardiogeneic Shock
SA339542RR_039Cardiogeneic Shock
SA339543RR_040Cardiogeneic Shock
SA339544RR_038Cardiogeneic Shock
SA339545RR_032Cardiogeneic Shock
SA339546RR_095Septic Shock
SA339547RR_096Septic Shock
SA339548RR_042Septic Shock
SA339549RR_007Septic Shock
SA339550RR_009Septic Shock
SA339551RR_008Septic Shock
SA339552RR_097Septic Shock
SA339553RR_006Septic Shock
SA339554RR_099Septic Shock
SA339555RR_106Septic Shock
SA339556RR_107Septic Shock
SA339557RR_109Septic Shock
SA339558RR_003Septic Shock
SA339559RR_105Septic Shock
SA339560RR_004Septic Shock
SA339561RR_100Septic Shock
SA339562RR_101Septic Shock
SA339563RR_102Septic Shock
SA339564RR_103Septic Shock
SA339565RR_098Septic Shock
SA339566RR_012Septic Shock
SA339567RR_026Septic Shock
SA339568RR_068Septic Shock
SA339569RR_069Septic Shock
SA339570RR_070Septic Shock
SA339571RR_065Septic Shock
SA339572RR_027Septic Shock
SA339573RR_002Septic Shock
SA339574RR_029Septic Shock
SA339575RR_028Septic Shock
SA339576RR_024Septic Shock
SA339577RR_033Septic Shock
SA339578RR_017Septic Shock
SA339579RR_016Septic Shock
SA339580RR_036Septic Shock
SA339581RR_014Septic Shock
SA339582RR_018Septic Shock
SA339583RR_035Septic Shock
SA339584RR_021Septic Shock
SA339585RR_074Septic Shock
SA339586RR_034Septic Shock
SA339587RR_037Septic Shock
Showing results 1 to 98 of 98

Collection:

Collection ID:CO003246
Collection Summary:Samples were collected from patients with sepsis, cardiogenic shock, bacteremia without sepsis, and ambulatory controls. All subjects (or legal surrogates) provided informed consent to participate in this study, which was approved by the Mass General Brigham IRB.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR003262
Treatment Summary:No treatment.

Sample Preparation:

Sampleprep ID:SP003260
Sampleprep Summary:30 µl of plasma sample was mixed with 137 µl of ice-cold acetonitrile for metabolite extraction. Samples were vortexed and incubated on ice for 30 mins. After centrifugation for 20 mins at 4°C and 21,000g, 90 µl of the sample was transferred to an autosampler glass vial for LCMS analysis.

Chromatography:

Chromatography ID:CH003897
Chromatography Summary:pH of Solvent A: pH9
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters XBridge BEH Amide (100 x 2.1mm,2.5um)
Column Temperature:27
Flow Gradient:0 mins 85%B, 0.5 min 85%B, 9 min 35%B, 11 min 2%B, 12min 2%B, 13.5 min 85%B, 14.6 min 85%B , 15 min 85%B with 0.420 mL/min to 18 min
Flow Rate:0.220 mL/min (0-15min), 0.420 mL/min (15-18min)
Solvent A:acetonitrile/water (5/95); 20mM ammonium acetate
Solvent B:100% acetonitrile
Chromatography Type:HILIC

Analysis:

Analysis ID:AN005149
Analysis Type:MS
Chromatography ID:CH003897
Num Factors:4
Num Metabolites:159
Units:AU
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