Summary of Study ST003196

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001992. The data can be accessed directly via it's Project DOI: 10.21228/M87J0K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST003196
Study Title	Unveiling cellular changes in leukaemia cell line HL-60 after cannabidiol treatment through lipidomics
Study Summary	The present study was aimed at revealing the metabolic changes that occurred in the cellular lipid pattern of acute and chronic myeloid leukaemia cells following treatment with cannabidiol (CBD). CBD is a non-psychoactive compound present in Cannabis sativa L., which has shown an antiproliferative action in these type of cancer cells, to determine significant alterations of the cell metabolism attributable to the induction of apoptosis, previously observed from in vitro studies. Control and treated cells of acute myeloid leukaemia (HL-60) were studied through an untargeted lipidomics approach. Treatment was carried out with CBD at a concentration of 10 μM for 48 h. After the extraction of the lipid content from cell lysates, the samples were analysed by UHPLC-QTOF-MS/MS both in the positive and the negative ionization modes.
Institute	Universidad CEU San Pablo
Laboratory	CEMBIO
Last Name	Garcia Fernández
First Name	Antonia
Address	Urb. Montepríncipe., Alcorcón, Madrid, 28925, Spain
Email	antogar@ceu.es
Phone	(+34) 91 372 47 69
Submit Date	2024-05-08
Num Groups	2
Total Subjects	10
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2024-09-17
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR001992
Project DOI:	doi: 10.21228/M87J0K
Project Title:	Unveiling cellular changes in leukaemia cell line HL-60 after cannabidiol treatment through lipidomics
Project Summary:	The present study was aimed at revealing the metabolic changes that occurred in the cellular lipid pattern of acute and chronic myeloid leukaemia cells following treatment with cannabidiol (CBD). CBD is a non-psychoactive compound present in Cannabis sativa L., which has shown an antiproliferative action in these type of cancer cells, to determine significant alterations of the cell metabolism attributable to the induction of apoptosis, previously observed from in vitro studies. Control and treated cells of acute myeloid leukaemia (HL-60) were studied through an untargeted lipidomics approach. Treatment was carried out with CBD at a concentration of 10 μM for 48 h. After the extraction of the lipid content from cell lysates, the samples were analysed by UHPLC-QTOF-MS/MS both in the positive and the negative ionization modes.
Institute:	Universidad CEU San Pablo
Laboratory:	CEMBIO
Last Name:	Garcia Fernández
First Name:	Antonia
Address:	Urb. Montepríncipe., Alcorcón, Madrid, 28925, Spain
Email:	antogar@ceu.es
Phone:	(+34) 91 372 47 69

Subject:

Subject ID:	SU003315
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Mammals

Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Treatment
SA347961	HL60_CBD1	CBD
SA347962	HL60_CBD5	CBD
SA347963	HL60_CBD4	CBD
SA347964	HL60_CBD2	CBD
SA347965	HL60_CBD3	CBD
SA347966	HL60_CTR5	CTR
SA347967	HL60_CTR4	CTR
SA347968	HL60_CTR1	CTR
SA347969	HL60_CTR2	CTR
SA347970	HL60_CTR3	CTR

Showing results 1 to 10 of 10

Showing results 1 to 10 of 10

Collection:

Collection ID:	CO003308
Collection Summary:	HL-60 cells were grown in RPMI medium supplemented with 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin. Cells were cultured in a humidiﬁed incubator at 37 °C with 95% humidity and 5% CO2.Cells were treated with CBD. Cells were washed with cold PBS, centrifuged and the pellets were stored at - 80°C until the day of the analysis.
Sample Type:	Leukemia cells

Treatment:

Treatment ID:	TR003324
Treatment Summary:	Cells were treated with 10 µM of CBD

Sample Preparation:

Sampleprep ID:	SP003322
Sampleprep Summary:	The samples were vortex-mixed for 2 min and 100 µL of cold MeOH (- 20°C) were added to each replicate for deproteinization. Ultrasound probe (UP 200 S Dr. Hielscher Ultrasonic Gmbh) was used (0.5 cycles; 80 amplitude x 16 times) to lyse the cells. Cell lysis was verified using a microscope. Twenty µL of the working solution of 20 mg/L of C17 sphinganine in methanol were added to 100 µL of each replicate. After that, 284 µL of methyl tert-butyl ether (MTBE) was added to each sample to extract hydrophobic compounds. Each replicate were vortex-mixed for 1 h (room temperature, 10,000 xg). Then, 71 µL of water was added to each sample and vortex-mixed for 1 min (25°C, 10,000 xg). Samples were centrifuged for 10 min at 16,000 xg at 4 °C. After centrifugation, 90 µL of the supernatant were transferred to vials for the analysis by LC-MS in the positive and in the negative ionization mode

Sampleprep ID:

SP003322

Sampleprep Summary:

The samples were vortex-mixed for 2 min and 100 µL of cold MeOH (- 20°C) were added to each replicate for deproteinization. Ultrasound probe (UP 200 S Dr. Hielscher Ultrasonic Gmbh) was used (0.5 cycles; 80 amplitude x 16 times) to lyse the cells. Cell lysis was verified using a microscope. Twenty µL of the working solution of 20 mg/L of C17 sphinganine in methanol were added to 100 µL of each replicate. After that, 284 µL of methyl tert-butyl ether (MTBE) was added to each sample to extract hydrophobic compounds. Each replicate were vortex-mixed for 1 h (room temperature, 10,000 xg). Then, 71 µL of water was added to each sample and vortex-mixed for 1 min (25°C, 10,000 xg). Samples were centrifuged for 10 min at 16,000 xg at 4 °C. After centrifugation, 90 µL of the supernatant were transferred to vials for the analysis by LC-MS in the positive and in the negative ionization mode

Chromatography:

Chromatography ID:	CH003971
Instrument Name:	Agilent 1290 Infinity II
Column Name:	Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
Column Temperature:	50ºC
Flow Gradient:	Started at 70% of B at 0-1 min, 86% B at 3.5-10 min, 100% B at 11-17 min. The starting conditions were recovered by minute 17.
Flow Rate:	0.6 mL/min
Solvent A:	90% Water, 10% Methanol; 10 mM ammonium acetate, 0.2 mM ammonium fluoride
Solvent B:	20% acetonitrile, 30% Methanol, 50% Isopropanol; 10 mM ammonium acetate, 0.2 mM ammonium fluoride
Chromatography Type:	Reversed phase

Analysis:

Analysis ID:	AN005245
Analysis Type:	MS
Chromatography ID:	CH003971
Has Rt:	1
Rt Units:	Minutes
Results File:	ST003196_AN005245_Results.txt
Units:	Corrected areas

Analysis ID:	AN005246
Analysis Type:	MS
Chromatography ID:	CH003971
Has Rt:	1
Rt Units:	Minutes
Results File:	ST003196_AN005246_Results.txt
Units:	Corrected areas