Summary of Study ST003216

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002006. The data can be accessed directly via it's Project DOI: 10.21228/M89R6J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003216
Study TitleBalancing brain metabolic states during sickness and recovery sleep
Study SummarySickness sleep and rebound following sleep deprivation share humoral signals including the rise of cytokines, in particular interleukins. Nevertheless, they represent unique physiological states with unique brain firing patterns and involvement of specific circuitry. Here we performed untargeted metabolomics of mouse cortex and hippocampus to uncover acute changes with sickness and rebound sleep as compared to normal daily sleep. We found that the three states are biochemically unique with larger differences in the cortex than in the hippocampus. Both sickness and rebound sleep shared an increase in tryptophan, with the highest levels during sickness. Surprisingly these two sleep states showed stark differences in terms of the energetic signature, with sickness impinging on glycolysis intermediates whilst rebound increased the triphosphorylated form of nucleotides. These findings indicate that rebound following sleep deprivation stimulates an energy rich state in the brain that is devoid during sickness sleep in line with the energy conservation hypothesis of sickness behavior.
Institute
University of Pennsylvania
Last NameSehgal
First NameAmita
AddressPerelman School of Medicine, University of Pennsylvania, 10-136 Smilow Research Center, 3400 Civic Center Blvd, Bldg 421, Philadelphia, PA 19104, USA
Emailamita@pennmedicine.upenn.edu
Phone215-898-2799
Submit Date2024-05-20
Raw Data AvailableYes
Raw Data File Type(s)mzML, raw(Thermo)
Analysis Type DetailLC-MS
Release Date2025-05-21
Release Version1
Amita Sehgal Amita Sehgal
https://dx.doi.org/10.21228/M89R6J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002006
Project DOI:doi: 10.21228/M89R6J
Project Title:Balancing brain metabolic states during sickness and recovery sleep
Project Summary:Sickness sleep and rebound following sleep deprivation share humoral signals including the rise of cytokines, in particular interleukins. Nevertheless, they represent unique physiological states with unique brain firing patterns and involvement of specific circuitry. Here we performed untargeted metabolomics of mouse cortex and hippocampus to uncover acute changes with sickness and rebound sleep as compared to normal daily sleep. We found that the three states are biochemically unique with larger differences in the cortex than in the hippocampus. Both sickness and rebound sleep shared an increase in tryptophan, with the highest levels during sickness. Surprisingly these two sleep states showed stark differences in terms of the energetic signature, with sickness impinging on glycolysis intermediates whilst rebound increased the triphosphorylated form of nucleotides. These findings indicate that rebound following sleep deprivation stimulates an energy rich state in the brain that is devoid during sickness sleep in line with the energy conservation hypothesis of sickness behavior.
Institute:University of Pennsylvania
Last Name:Sehgal
First Name:Amita
Address:Perelman School of Medicine, University of Pennsylvania, 10-136 Smilow Research Center, 3400 Civic Center Blvd, Bldg 421, Philadelphia, PA 19104, USA
Email:amita@pennmedicine.upenn.edu
Phone:215-898-2799

Subject:

Subject ID:SU003335
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Treatment
SA351778A1Cortex Base Line
SA351779A3Cortex Base Line
SA351780A4Cortex Base Line
SA351781A2Cortex Base Line
SA351782C2Cortex LPS
SA351783C4Cortex LPS
SA351784C1Cortex LPS
SA351785C3Cortex LPS
SA351786B2Cortex Sleep Deprivation
SA351787B4Cortex Sleep Deprivation
SA351788B1Cortex Sleep Deprivation
SA351789B3Cortex Sleep Deprivation
SA351790AA2Hippocampus Base Line
SA351791AA4Hippocampus Base Line
SA351792AA1Hippocampus Base Line
SA351793AA3Hippocampus Base Line
SA351794CC1Hippocampus LPS
SA351795CC2Hippocampus LPS
SA351796CC3Hippocampus LPS
SA351797CC4Hippocampus LPS
SA351798BB4Hippocampus Sleep Deprivation
SA351799BB3Hippocampus Sleep Deprivation
SA351800BB2Hippocampus Sleep Deprivation
SA351801BB1Hippocampus Sleep Deprivation
SA351802QC-5n/a n/a
SA351803QC-4n/a n/a
SA351804QC-7n/a n/a
SA351805QC-6n/a n/a
Showing results 1 to 28 of 28

Collection:

Collection ID:CO003328
Collection Summary:6 hours after lights on, the middle of the rest phase in mice, animals were euthanized using isoflurane anesthesia followed by cervical dislocation. Cortex and hippocampus were collected, cleaned in ice cold PBS, gently dried and flash frozen.
Sample Type:Brain

Treatment:

Treatment ID:TR003344
Treatment Summary:Base Line: mice were undisturbed before ZT6 collection, meaning 6h after Light on. SD: 4 hours of gentle handling sleep deprivation (SD) from ZT0 to ZT4 followed by 2 h of recovery sleep leading to ZT6 collection. LPS: intraperitoneal injection of 0.5 mgKg-1 lipopolysaccharide (LPS) at ZT4 followed by 2h of recovery sleep leading to ZT6 collection.

Sample Preparation:

Sampleprep ID:SP003342
Sampleprep Summary:Frozen samples were weighed (10 mg) and homogenized using a mortar and pestle with 0.45 ml cold extraction solution (80% methanol, 20% water with heavy-labeled internal standard mix). Samples were placed on dry ice for 30 min then centrifuged at 10,000 x g for 15 min at 4 °C. Supernatants were collected, and the pellet was re-extracted with an additional 200 μl of cold extraction solution. The supernatants from both extractions were combined, clarified again by centrifugation, and stored at -80 °C in cryovials prior to analysis. A quality control (QC) sample was generated by pooling equal volumes of all samples immediately before LC-MS analysis.

Chromatography:

Chromatography ID:CH003990
Chromatography Summary:Hydrophilic interaction liquid chromatography (HILIC) was performed at 0.2 ml/min on a ZIC-pHILIC column (2.1 mm × 150 mm, 5 μm particle size, EMD Millipore) at 45 °C. Solvent A was 20 mM ammonium carbonate, 0.1% ammonium hydroxide, pH 9.2, and solvent B was acetonitrile. The gradient was 85% B for 2 min, 85% B to 20% B over 15 min, 20% B to 85% B over 0.1 min, and 85% B for 8.9 min. The autosampler was held at 4 °C. For each analysis, 4 µl of sample was injected.
Instrument Name:Thermo Vanquish
Column Name:SeQuant ZIC- pHILIC (150 x 2.1mm,5um)
Column Temperature:45
Flow Gradient:85% B for 2 min, 85% B to 20% B over 15 min, 20% B to 85% B over 0.1 min, and 85% B for 8.9 min
Flow Rate:0.2 mL/min
Solvent A:100% Water; 20 mM ammonium carbonate, 0.1% ammonium hydroxide, pH 9.2
Solvent B:100% Acetonitrile
Chromatography Type:HILIC

Analysis:

Analysis ID:AN005273
Analysis Type:MS
Chromatography ID:CH003990
Num Factors:7
Num Metabolites:88
Rt Units:Minutes
Units:Peak Area
  
Analysis ID:AN005274
Analysis Type:MS
Chromatography ID:CH003990
Num Factors:7
Num Metabolites:64
Rt Units:Minutes
Units:Peak Area
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