Summary of Study ST003293

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002045. The data can be accessed directly via it's Project DOI: 10.21228/M88N7K This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003293
Study TitleHigh Fat Feeding Alters Circulating Triglyceride Profile by Decreasing SCD Activity and Depleting omega-3 Fatty Acids
Study SummaryThe study was to examine, in control and high-fat-fed rats, the effects of acute feeding (a single meal) on circulating lipid profiles. Acute feeding is expected to impact circulating lipid profiles due to lipids in chylomicron derived from the diet, and the acute feeding response may be altered with a HFD to account for some of the chronic effects of a HFD. The study was to examine the effects of a high fat diet (HFD) on circulating TG profiles in rats and test the hypothesis that a HFD reduces TGs composed of long-chain PUFAs, as previously seen in rats maintained on a HNaLK diet. Thus, we performed lipidomics analysis on plasma samples collected both in the pre- and postprandial states in the control and HFD groups. Lipidomics analysis was combined with general metabolomics analysis, which provides measurements of saturated FFAs and other metabolic indicators such as glycerol (as lipolysis indicator) or 3-hydroxybutyrate (ketosis indicator), not measured in our LC-MS-based lipidomics assay. The results provided novel insights into the differential behaviors of individual TGs and identified specific changes (i.e., decreased stearoyl-CoA desaturase [SCD] activity and depletion of omega-3 FFAs) associated with short-term high-fat feeding.
Institute
University of Southern California
DepartmentKeck School of Medicine USC
Last NameYoun
First NameJang-Hyun
AddressCSC 214, 2250 Alcazar Street, Health Sciences Campus, Los Angeles CA 90033
Emailyoun@usc.edu
Phone5307528129
Submit Date2024-06-26
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-07-25
Release Version1
Jang-Hyun Youn Jang-Hyun Youn
https://dx.doi.org/10.21228/M88N7K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002045
Project DOI:doi: 10.21228/M88N7K
Project Title:High Fat Feeding Alters Circulating Triglyceride Profile by Decreasing SCD Activity and Depleting omega-3 Fatty Acids
Project Summary:The study was to examine, in control and high-fat-fed rats, the effects of acute feeding (a single meal) on circulating lipid profiles. Acute feeding is expected to impact circulating lipid profiles due to lipids in chylomicron derived from the diet, and the acute feeding response may be altered with a HFD to account for some of the chronic effects of a HFD. The study was to examine the effects of a high fat diet (HFD) on circulating TG profiles in rats and test the hypothesis that a HFD reduces TGs composed of long-chain PUFAs, as previously seen in rats maintained on a HNaLK diet. Thus, we performed lipidomics analysis on plasma samples collected both in the pre- and postprandial states in the control and HFD groups. Lipidomics analysis was combined with general metabolomics analysis, which provides measurements of saturated FFAs and other metabolic indicators such as glycerol (as lipolysis indicator) or 3-hydroxybutyrate (ketosis indicator), not measured in our LC-MS-based lipidomics assay. The results provided novel insights into the differential behaviors of individual TGs and identified specific changes (i.e., decreased stearoyl-CoA desaturase [SCD] activity and depletion of omega-3 FFAs) associated with short-term high-fat feeding.
Institute:University of Southern California
Department:Keck School of Medicine USC
Last Name:Youn
First Name:Jang-Hyun
Address:CSC 214, 2250 Alcazar Street, Health Sciences Campus, Los Angeles CA 90033
Email:youn@usc.edu
Phone:5307528129

Subject:

Subject ID:SU003413
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Treatment sub treatment
SA3568151383 PPlasma CtrlDiet AfterM
SA3568161412 PPlasma CtrlDiet AfterM
SA3568171407 PPlasma CtrlDiet AfterM
SA3568181404 PPlasma CtrlDiet AfterM
SA3568191399 PPlasma CtrlDiet AfterM
SA3568201396 PPlasma CtrlDiet AfterM
SA3568211391 PPlasma CtrlDiet AfterM
SA3568221388 PPlasma CtrlDiet AfterM
SA3568231375 PPlasma CtrlDiet AfterM
SA3568241380 PPlasma CtrlDiet AfterM
SA3568251378 PPlasma CtrlDiet BeforeM
SA3568261373 PPlasma CtrlDiet BeforeM
SA3568271410 PPlasma CtrlDiet BeforeM
SA3568281381 PPlasma CtrlDiet BeforeM
SA3568291402 PPlasma CtrlDiet BeforeM
SA3568301397 PPlasma CtrlDiet BeforeM
SA3568311394 PPlasma CtrlDiet BeforeM
SA3568321389 PPlasma CtrlDiet BeforeM
SA3568331386 PPlasma CtrlDiet BeforeM
SA3568341405 PPlasma CtrlDiet BeforeM
SA3568351411 PPlasma HFD AfterM
SA3568361408 PPlasma HFD AfterM
SA3568371403 PPlasma HFD AfterM
SA3568381395 PPlasma HFD AfterM
SA3568391392 PPlasma HFD AfterM
SA3568401387 PPlasma HFD AfterM
SA3568411384 PPlasma HFD AfterM
SA3568421379 PPlasma HFD AfterM
SA3568431400 PPlasma HFD AfterM
SA3568441376 PPlasma HFD AfterM
SA3568451382 PPlasma HFD BeforeM
SA3568461406 PPlasma HFD BeforeM
SA3568471374 PPlasma HFD BeforeM
SA3568481377 PPlasma HFD BeforeM
SA3568491409 PPlasma HFD BeforeM
SA3568501385 PPlasma HFD BeforeM
SA3568511401 PPlasma HFD BeforeM
SA3568521393 PPlasma HFD BeforeM
SA3568531398 PPlasma HFD BeforeM
SA3568541390 PPlasma HFD BeforeM
SA356855PoolQC009Plasma QC1 QC1
SA356856PoolQC007Plasma QC1 QC1
SA356857PoolQC006Plasma QC1 QC1
SA356858PoolQC008Plasma QC1 QC1
SA356859PoolQC004Plasma QC1 QC1
SA356860PoolQC003Plasma QC1 QC1
SA356861PoolQC002Plasma QC1 QC1
SA356862PoolQC001Plasma QC1 QC1
SA356863PoolQC005Plasma QC1 QC1
SA356864MtdBlank001QC2 QC2 QC2
SA356865MtdBlank002QC2 QC2 QC2
SA356866MtdBlank003QC2 QC2 QC2
SA356867MtdBlank004QC2 QC2 QC2
SA356868MtdBlank005QC2 QC2 QC2
SA356869MtdBlank006QC2 QC2 QC2
SA356870MtdBlank007QC2 QC2 QC2
SA356871MtdBlank008QC2 QC2 QC2
SA356872MtdBlank009QC2 QC2 QC2
Showing results 1 to 58 of 58

Collection:

Collection ID:CO003406
Collection Summary:blood samples were collected through cardiac puncture. Blood samples were rapidly spun, and plasmas were isolated and stored at -80°C until analysis
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR003422
Treatment Summary:control low fat diet (LFD) before meal, control low fat diet after meal, high fat diet (HFD) before meal, high fat diet after meal

Sample Preparation:

Sampleprep ID:SP003420
Sampleprep Summary:SOP blood-lipidomics-02272019, Matyash extraction and the organic layer was dried, reconstituted, and injected
Sampleprep Protocol Filename:SOP_blood-lipidomics-02272019.pdf

Combined analysis:

Analysis ID AN005392 AN005393
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Agilent 1290
Column Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak height Peak height

Chromatography:

Chromatography ID:CH004088
Instrument Name:Agilent 1290
Column Name:Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um)
Column Temperature:65
Flow Gradient:0 min 15% B, 0–0.75 min 30% B, 0.75-0.98 min 48% B, 0.98-4.00 min 82% B, 4.00-4.13 min 99% B, 4.13-4.50 min 99% B, 4.50-4.58 min 15% B, 4.58-5.50 min 15% B
Flow Rate:0.8 mL/min
Solvent A:60% acetonitrile/40% water; 10 mM Ammonium formate; 0.1% Formic acid
Solvent B:90% Isopropyl alcohol/10% acetonitrile; 10 mM Ammonium Formate; 0.1% Formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH004089
Instrument Name:Agilent 1290
Column Name:Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um)
Column Temperature:65
Flow Gradient:0 min 15% B, 0–0.75 min 30% B, 0.75-0.98 min 48% B, 0.98-4.00 min 82% B, 4.00-4.13 min 99% B, 4.13-4.50 min 99% B, 4.50-4.58 min 15% B, 4.58-5.50 min 15% B
Flow Rate:0.8 mL/min
Solvent A:60% acetonitrile/40% water; 10 mM Ammonium Acetate
Solvent B:90% Isopropyl alcohol/10% acetonitrile; 10 mM Ammonium Acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS005119
Analysis ID:AN005392
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:The quadrupole/time-of-flight (QTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 65–1700. Equipped with a dual JetStream Source. modes producing both unique and complementary spectra. Instrument parameters are as follows (positive mode) Gas Temp 320°C, Gas Flow 8 l/min, Nebulizer 35 psi, Sheath Gas 350°C, Sheath Gas Flow 11, Capillary Voltage 3500 V, Nozzle Voltage 1000V, Fragmentor 175V, Skimmer 65V. Data (both profile and centroid) are collected at a rate of 5 scans per second. In negative ion mode, Gas Temp 360°C, Gas Flow 9 l/min, Fragmentor 120V, with the other parameters identical to positive ion mode. A reference solution generating ions of 121.050 and 922.007 m/z in positive mode and 119.036 and 966.0007 m/z in negative mode, and these are used for continuous mass correction.
Ion Mode:POSITIVE
  
MS ID:MS005120
Analysis ID:AN005393
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:The quadrupole/time-of-flight (QTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 65–1700. Equipped with a dual JetStream Source. modes producing both unique and complementary spectra. Instrument parameters are as follows (positive mode) Gas Temp 320°C, Gas Flow 8 l/min, Nebulizer 35 psi, Sheath Gas 350°C, Sheath Gas Flow 11, Capillary Voltage 3500 V, Nozzle Voltage 1000V, Fragmentor 175V, Skimmer 65V. Data (both profile and centroid) are collected at a rate of 5 scans per second. In negative ion mode, Gas Temp 360°C, Gas Flow 9 l/min, Fragmentor 120V, with the other parameters identical to positive ion mode. A reference solution generating ions of 121.050 and 922.007 m/z in positive mode and 119.036 and 966.0007 m/z in negative mode, and these are used for continuous mass correction.
Ion Mode:NEGATIVE
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