Summary of Study ST003364

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002090. The data can be accessed directly via it's Project DOI: 10.21228/M8G24F This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003364
Study TitleDeep lipidomic profiling reveals sex dimorphism of lipid metabolism in fibro-calcific aortic valve disease
Study SummaryIn-depth lipidomics profiling was performed on human tricuspid and bicuspid aortic valve tissue at different pathological stages, including mildly diseased, fibrotic and calcific tissue sections. Untargeted and targeted analysis were employed to enable high identification coverage and accurate quantification of lipids. Chromatographic separation modes (C18 RPC, C30 RPC) was combined with MS analysis using various acquisition modes (full MS, DDA, SRM) to allow for the identification of 1084 lipid molecular species across 28 lipid subclasses, of which 480 lipids were semi-absolutely quantified.
Institute
Technical University Dresden
Last NameFedorova
First NameMaria
AddressTatzberg 47/49, Dresden, Saxony, 01307, Germany
Emailmaria.fedorova@tu-dresden.de
Phone+49 351 796 36617
Submit Date2024-07-18
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-08-15
Release Version1
Maria Fedorova Maria Fedorova
https://dx.doi.org/10.21228/M8G24F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002090
Project DOI:doi: 10.21228/M8G24F
Project Title:Deep lipidomic profiling reveals sex dimorphism of lipid metabolism in fibro-calcific aortic valve disease
Project Summary:In-depth lipidomics profiling was performed on human tricuspid and bicuspid aortic valve tissue at different pathological stages, including mildly diseased, fibrotic and calcific tissue sections. Untargeted and targeted analysis were employed to enable high identification coverage and accurate quantification of lipids. Chromatographic separation modes (C18 RPC, C30 RPC) was combined with MS analysis using various acquisition modes (full MS, DDA, SRM) to allow for the identification of 1084 lipid molecular species across 28 lipid subclasses, of which 480 lipids were semi-absolutely quantified.
Institute:Technical University Dresden
Last Name:Fedorova
First Name:Maria
Address:Tatzberg 47/49, Dresden, Saxony, 01307, Germany
Email:maria.fedorova@tu-dresden.de
Phone:+49 351 796 36617

Subject:

Subject ID:SU003485
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Analysis method Extraction method Morphology Sex Pathology
SA366398A_cal1_1.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366399A_cal1_3.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366400A_cal1_2.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366401A_cal1_2.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366402A_cal1_2.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366403A_cal1_1.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366404A_cal_tQC3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366405A_cal1_1.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366406A_cal1_0.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366407A_cal1_0.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366408A_cal1_0.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366409A_cal_tQC2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366410A_cal_tQC1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366411A_cal1_3.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366412A_cal1_3.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366413A_cal1_5.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366414A_cal1_4.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366415A_cal2_2.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366416A_cal2_6.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366417A_cal2_6.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366418A_cal2_6.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366419A_cal2_5.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366420A_cal2_5.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366421A_cal2_5.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366422A_cal2_4.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366423A_cal2_4.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366424A_cal2_4.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366425A_cal2_3.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366426A_cal2_3.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366427A_cal2_2.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366428A_cal2_2.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366429A_cal1_4.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366430A_cal2_1.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366431A_cal2_1.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366432A_cal2_1.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366433A_cal2_0.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366434A_cal2_0.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366435A_cal2_0.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366436A_cal1_6.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366437A_cal1_6.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366438A_cal1_6.1Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366439A_cal1_5.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366440A_cal1_5.2Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366441A_cal1_4.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366442A_cal2_3.3Aortic valve tissue acidified MeOH/CHCl3 extraction and targeted SRM acidified MeOH/CHCl3 mixed mixed mixed
SA366033A_POST_pool_3.1Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366034A_PRE_pool_3.3Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366035A_POST_pool_3.2Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366036A_POST_pool_2.3Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366037A_POST_pool_2.2Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366038A_POST_pool_2.1Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366039A_POST_pool_1.3Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366040A_POST_pool_1.2Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366041A_POST_pool_1.1Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366042A_PRE_pool_3.2Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366043A_PRE_pool_0.1Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366044A_PRE_pool_3.1Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366045A_PRE_pool_2.3Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366046A_PRE_pool_2.2Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366047A_PRE_pool_2.1Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366048A_PRE_pool_1.3Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366049A_PRE_pool_1.2Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366050A_PRE_pool_1.1Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366051A_PRE_pool_0.3Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366052A_POST_pool_3.3Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366053A_PRE_pool_0.2Aortic valve tissue Assessment of PA and LPA recovery rates acidified MeOH/CHCl3 mixed mixed mixed
SA366012F_PRE_pool_3.3Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366013F_PRE_pool_0.3Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366014F_PRE_pool_1.1Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366015F_PRE_pool_1.2Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366016F_PRE_pool_1.3Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366017F_PRE_pool_2.1Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366018F_PRE_pool_2.3Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366019F_PRE_pool_3.1Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366020F_PRE_pool_3.2Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366021F_POST_pool_1.1Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366022F_PRE_pool_0.1Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366023F_POST_pool_1.2Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366024F_POST_pool_1.3Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366025F_POST_pool_2.1Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366026F_POST_pool_2.2Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366027F_POST_pool_2.3Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366028F_POST_pool_3.1Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366029F_POST_pool_3.2Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366030F_POST_pool_3.3Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366031F_PRE_pool_0.2Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366032F_PRE_pool_2.2Aortic valve tissue Assessment of PA and LPA recovery rates Folch mixed mixed mixed
SA366054F_cal-p_3.1Aortic valve tissue Folch extraction and untargeted MS10 Folch mixed mixed mixed
SA366055F_cal-p_3.2Aortic valve tissue Folch extraction and untargeted MS11 Folch mixed mixed mixed
SA366056F_cal-p_3.3Aortic valve tissue Folch extraction and untargeted MS12 Folch mixed mixed mixed
SA366057F_cal-p_4.1Aortic valve tissue Folch extraction and untargeted MS13 Folch mixed mixed mixed
SA366058F_cal-p_4.2Aortic valve tissue Folch extraction and untargeted MS14 Folch mixed mixed mixed
SA366059F_cal-p_4.3Aortic valve tissue Folch extraction and untargeted MS15 Folch mixed mixed mixed
SA366060F_cal-p_5.1Aortic valve tissue Folch extraction and untargeted MS16 Folch mixed mixed mixed
SA366061F_cal-p_5.2Aortic valve tissue Folch extraction and untargeted MS17 Folch mixed mixed mixed
SA366062F_cal-p_5.3Aortic valve tissue Folch extraction and untargeted MS18 Folch mixed mixed mixed
SA366063F_cal-p_6.1Aortic valve tissue Folch extraction and untargeted MS19 Folch mixed mixed mixed
SA366064F_cal-p_0.1Aortic valve tissue Folch extraction and untargeted MS1 Folch mixed mixed mixed
SA366065F_cal-p_6.2Aortic valve tissue Folch extraction and untargeted MS20 Folch mixed mixed mixed
SA366066F_cal-p_6.3Aortic valve tissue Folch extraction and untargeted MS21 Folch mixed mixed mixed
Showing page 1 of 5     Results:    1  2  3  4  5  Next     Showing results 1 to 100 of 431

Collection:

Collection ID:CO003478
Collection Summary:CAVD tissues were obtained from 55 patients undergoing aortic valve replacement for severe aortic stenosis. For all human tissue samples used in this study, tissue collection was approved by the local Ethical Committee (Medical Faculty, University Leipzig, registration number 128/19-ek) and all patients gave written informed consent in accordance with the Declaration of Helsinki. All samples showed macroscopic calcification as a sign of progressed FCAVD. Tissue samples were dissected into mildly diseased, fibrotic and calcific pieces on-site and flash frozen in liquid nitrogen. The deep frozen tissue sections were crushed into a thin powder in a percussion mortar in liquid nitrogen and stored at -80°C until further analysis.
Sample Type:Aortic valve tissue
Collection Location:Leipzig, Germany
Storage Conditions:-80?
Storage Vials:Plastic tubes
Additives:none

Treatment:

Treatment ID:TR003494
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP003492
Sampleprep Summary:Aortic valve tissue was spiked with tailored mixtures of internal standards and lipids were extracted either using the Folch method or by acidified MeOH/CHCl3 lipid extraction.
Processing Storage Conditions:4?
Extraction Method:Folch lipid extraction or acidified MeOH/CHCl3 lipid extraction
Extract Storage:-80?
Sample Derivatization:none
Sample Spiking:aortic valve tissue specific internal standard mix

Combined analysis:

Analysis ID AN005510 AN005511 AN005512 AN005513
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish Thermo Vanquish Thermo Vanquish
Column Thermo Accucore C30 (150 x 2.1mm,2.6um) Thermo Accucore C30 (150 x 2.1mm,2.6um) Thermo Accucore C30 (150 x 2.1mm,2.6um) Thermo Accucore C18 (150 x 2.1mm,2.6um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Triple quadrupole
MS instrument name Thermo Exploris 240 Orbitrap Thermo Exploris 240 Orbitrap Thermo Exploris 240 Orbitrap Thermo TSQ Altis Plus
Ion Mode NEGATIVE UNSPECIFIED POSITIVE NEGATIVE
Units min pmol/mg wet tissue weight pmol/mg wet tissue weight Peak area

Chromatography:

Chromatography ID:CH004189
Chromatography Summary:Four liquid chromatography methods were employed to allow optimal lipidome separation to enhance coverage of lipid species identifications and fast quantification. All details on the respective methods are provided in the attached methods document
Methods Filename:FCAVD_methods.pdf
Instrument Name:Thermo Vanquish
Column Name:Thermo Accucore C30 (150 x 2.1mm,2.6um)
Column Temperature:50 °C
Flow Gradient:0-20 min – 10 to 86% B, 20-22 min – 86 to 95% B, 22-26 min – 95% B, 26-26.1 min – 95 to 10% B, followed by 7.9 min re-equilibration at 10% B.
Flow Rate:0.3 mL/min
Solvent A:50% acetonitrile/50% water; 5 mM ammonium formate; 0.1% formic acid
Solvent B:85% isopropanol/10% acetonitrile/5% water; 5 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH004190
Chromatography Summary:Four liquid chromatography methods were employed to allow optimal lipidome separation to enhance coverage of lipid species identifications and fast quantification. All details on the respective methods are provided in the attached methods document
Methods Filename:FCAVD_methods.pdf
Instrument Name:Thermo Vanquish
Column Name:Thermo Accucore C30 (150 x 2.1mm,2.6um)
Column Temperature:50 °C
Flow Gradient:0-20 min – 10 to 80% B, 20-37 min – 80 to 95% B, 37-41 min – 95% to 100% B, 41-49 min – 100% B, 49-49.1 100% to 10% B, followed by 7.9 min re-equilibration at 10%
Flow Rate:0.3 mL/min
Solvent A:50% acetonitrile/50% water; containing 5 mM ammonium formate and 0.1% (v/v) formic acid
Solvent B:85% isopropanol/10% acetonitrile/5% water; 5 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH004191
Chromatography Summary:Four liquid chromatography methods were employed to allow optimal lipidome separation to enhance coverage of lipid species identifications and fast quantification. All details on the respective methods are provided in the attached methods document
Methods Filename:FCAVD_methods.pdf
Instrument Name:Thermo Vanquish
Column Name:Thermo Accucore C30 (150 x 2.1mm,2.6um)
Column Temperature:50 °C
Flow Gradient:0-10 min – 30 to 80% B, 10-27 min – 80 to 95% B, 27-31 min – 95% to 100% B, 31-39 min – 100% B, 39-39.1 100% to 10% B, followed by 7.9 min re-equilibration at 10% B.
Flow Rate:0.3 mL/min
Solvent A:50% acetonitrile/50% water; containing 5 mM ammonium formate and 0.1% (v/v) formic acid
Solvent B:85% isopropanol/10% acetonitrile/5% water; 5 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH004192
Chromatography Summary:Four liquid chromatography methods were employed to allow optimal lipidome separation to enhance coverage of lipid species identifications and fast quantification. All details on the respective methods are provided in the attached methods document
Methods Filename:FCAVD_methods.pdf
Instrument Name:Thermo Vanquish
Column Name:Thermo Accucore C18 (150 x 2.1mm,2.6um)
Column Temperature:50 °C
Flow Gradient:0-7 min – 30 to 85% B, 7-8 min – 85 to 95% B, 8-10 min – 95% B, 10-10.1 min – 95% to 30% B, followed by 4.9 min re-equilibration at 10% B.
Flow Rate:0.3 mL/min
Solvent A:50% acetonitrile/50% water; 5 mM ammonium formate; 0.1% formic acid
Solvent B:85% isopropanol/10% acetonitrile/5% water; 5 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS005235
Analysis ID:AN005510
Instrument Name:Thermo Exploris 240 Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:DDA with with inclusion list for the assessment of PA and LPA recovery rates of Folch and acidified MeOH/CHCl3 lipid extraction in negative ion mode
Ion Mode:NEGATIVE
  
MS ID:MS005236
Analysis ID:AN005511
Instrument Name:Thermo Exploris 240 Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:(1) Untargeted lipidomics for human AV lipids identification in DDA mode (2) Serial dilutions of aortic valve-tailored ISTD mixture in pooled sample, extracted by either Folch or acidified MeOH/CHCl3 lipid extraction, used for seven-point calibration curves generation in MS1 or SRM mode
Ion Mode:UNSPECIFIED
  
MS ID:MS005237
Analysis ID:AN005512
Instrument Name:Thermo Exploris 240 Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Untargeted lipidomics for human AV lipids quantification in MS1 positive ion mode
Ion Mode:POSITIVE
  
MS ID:MS005238
Analysis ID:AN005513
Instrument Name:Thermo TSQ Altis Plus
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:(1) Serial dilutions of aortic valve-tailored ISTD mixture in pooled sample, extracted by either Folch or acidified MeOH/CHCl3 lipid extraction, used for seven-point calibration curves generation in MS1 or SRM mode (2) Targeted lipidomics for human AV lipids quantification in SRM negative ion mode
Ion Mode:NEGATIVE
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