Summary of Study ST003391

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002085. The data can be accessed directly via it's Project DOI: 10.21228/M83R6P This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003391
Study TitleImpact of high-fat diet enriched in cis or trans fatty acids and myriocin on plasma sphingolipids in Ldlr-/- mice
Study SummaryWe analyzed sphingolipids in the plasma of Ldlr-/- mice fed 1) Cis HFD, 2) Cis HFD + Myriocin, 3) Trans HFD, or 4) Trans HFD + Myriocin. We aimed to determine how dietary cis and trans monounsaturated fatty acids impact circulating sphingolipids while pharmacologically inhibiting the initial rate-limiting enzyme of sphingolipid biosynthesis, serine palmitoyltransferase (SPT), via myriocin.
Institute
Salk Institute for Biological Studies
Last NameGengatharan
First NameJivani
Address10010 N Torrey Pines Rd, La Jolla, CA, 92037, USA
Emailjivani14@gmail.com
Phone(858) 453-4100
Submit Date2024-07-31
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2024-08-12
Release Version1
Jivani Gengatharan Jivani Gengatharan
https://dx.doi.org/10.21228/M83R6P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002085
Project DOI:doi: 10.21228/M83R6P
Project Title:Altered sphingolipid biosynthetic flux and lipoprotein trafficking contribute to trans fat-induced atherosclerosis
Project Summary:The goal of the project is to determine the role of sphingolipid metabolism in atherosclerosis induced by dietary trans fat. We analyzed lipid metabolites in Huh7 cells following various fatty acid treatments, with specific focus on cis and trans unsaturated fatty acids. Additionally, we analyzed lipid metabolites in plasma and liver of Ldlr-/- mice fed high-fat diets enriched in cis or trans fatty acids in the presence or absence of myriocin, a pharmacological inhibitor of Serine palmitoyltransferase (SPT), the initial rate-limiting enzyme of sphingolipid biosynthesis.
Institute:Salk Institute for Biological Studies
Last Name:Gengatharan
First Name:Jivani
Address:10010 N Torrey Pines Rd, La Jolla, CA, 92037, USA
Email:jivani14@gmail.com
Phone:(858) 453-4100

Subject:

Subject ID:SU003514
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Group
SA368313Plasma_C8_CisHFD_33Blood plasma Cis HFD
SA368314Plasma_C8_CisHFD_12Blood plasma Cis HFD
SA368315Plasma_C8_CisHFD_35Blood plasma Cis HFD
SA368316Plasma_C8_CisHFD_34Blood plasma Cis HFD
SA368317Plasma_C8_CisHFD_11Blood plasma Cis HFD
SA368318Plasma_C8_CisHFD_32Blood plasma Cis HFD
SA368319Plasma_C8_CisHFD_13Blood plasma Cis HFD
SA368320Plasma_C8_CisHFD_31Blood plasma Cis HFD
SA368321Plasma_C8_CisHFD_14Blood plasma Cis HFD
SA368322Plasma_C8_CisHFD_15Blood plasma Cis HFD
SA368323Plasma_C8_CisHFDMyr_101Blood plasma Cis HFD + Myriocin
SA368324Plasma_C8_CisHFDMyr_102Blood plasma Cis HFD + Myriocin
SA368325Plasma_C8_CisHFDMyr_104Blood plasma Cis HFD + Myriocin
SA368326Plasma_C8_CisHFDMyr_105Blood plasma Cis HFD + Myriocin
SA368327Plasma_C8_CisHFDMyr_141Blood plasma Cis HFD + Myriocin
SA368328Plasma_C8_CisHFDMyr_143Blood plasma Cis HFD + Myriocin
SA368329Plasma_C8_CisHFDMyr_144Blood plasma Cis HFD + Myriocin
SA368330Plasma_C8_CisHFDMyr_145Blood plasma Cis HFD + Myriocin
SA368331Plasma_C8_CisHFDMyr_142Blood plasma Cis HFD + Myriocin
SA368332Plasma_C8_TransHFD_82Blood plasma Trans HFD
SA368333Plasma_C8_TransHFD_85Blood plasma Trans HFD
SA368334Plasma_C8_TransHFD_84Blood plasma Trans HFD
SA368335Plasma_C8_TransHFD_83Blood plasma Trans HFD
SA368336Plasma_C8_TransHFD_45Blood plasma Trans HFD
SA368337Plasma_C8_TransHFD_81Blood plasma Trans HFD
SA368338Plasma_C8_TransHFD_44Blood plasma Trans HFD
SA368339Plasma_C8_TransHFD_43Blood plasma Trans HFD
SA368340Plasma_C8_TransHFD_41Blood plasma Trans HFD
SA368341Plasma_C8_TransHFD_42Blood plasma Trans HFD
SA368342Plasma_C8_TransHFDMyr_61Blood plasma Trans HFD + Myriocin
SA368343Plasma_C8_TransHFDMyr_62Blood plasma Trans HFD + Myriocin
SA368344Plasma_C8_TransHFDMyr_63Blood plasma Trans HFD + Myriocin
SA368345Plasma_C8_TransHFDMyr_64Blood plasma Trans HFD + Myriocin
SA368346Plasma_C8_TransHFDMyr_65Blood plasma Trans HFD + Myriocin
SA368347Plasma_C8_TransHFDMyr_91Blood plasma Trans HFD + Myriocin
SA368348Plasma_C8_TransHFDMyr_92Blood plasma Trans HFD + Myriocin
SA368349Plasma_C8_TransHFDMyr_93Blood plasma Trans HFD + Myriocin
SA368350Plasma_C8_TransHFDMyr_94Blood plasma Trans HFD + Myriocin
SA368351Plasma_C8_TransHFDMyr_95Blood plasma Trans HFD + Myriocin
Showing results 1 to 39 of 39

Collection:

Collection ID:CO003507
Collection Summary:Blood was collected in EDTA-coated tubes (Sarstedt Inc.) and centrifuged at 2000g for 5 min. The plasma supernatant was transferred to a new Eppendorf tube and stored at -80°C until analysis.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR003523
Treatment Summary:Four-five-week-old Ldlr-/- C57BL/6J male and female mice (JAX# 002207) were fed with irradiated 60% high fat diets (HFD) prepared by Dyets for 16 weeks. These diets include Cis Unsaturated HFD (105063GI), Cis Unsaturated HFD with 2.2 mg/kg Myriocin Added (105064GI), Trans Unsaturated HFD (105061GI), and Trans Unsaturated HFD with 2.2 mg/kg Myriocin Added (105061GI). The Trans Unsaturated HFD was designed with 100% Primex, a partially hydrogenated vegetable oil, and the Cis Unsaturated HFD was designed with 34% lard and 66% olive oil.

Sample Preparation:

Sampleprep ID:SP003521
Sampleprep Summary:Plasma (100 µl) was spiked with the following internal standards: 20 pmol sphinganine-d7 (Avanti Polar Lipids, Cat# 860658), deoxysphinganine-d3 (Avanti Polar Lipids, Cat# 860474), 100 pmol d18:0-d7/13:0 dihydroceramide (Avanti Polar Lipids, Cat# 330726), 200 pmol d18:1-d7/15:0 ceramide (Avanti Polar Lipids, Cat# 860681), 100 pmol d18:1-d7/15:0 glucosylceramide (Avanti Polar Lipids, Cat# 330729), 100 pmol d18:1-d7/15:0 lactosylceramide (Avanti Polar Lipids, Cat# 330727), 200 pmol sphingosine-d7 (Avanti Polar Lipids, Cat# 860657), and 200 pmol d18:1/18:1-d9 sphingomyelin (Avanti Polar Lipids, Cat# 791649). 0.5 mL methanol, 0.5 mL H2O, and 1 mL chloroform were added directly. Samples were vortexed for 5 min and centrifuged for 5 min at 4 ˚C at 15,000g. The organic phase was collected and 2 μL of formic acid was added to the remaining polar phase which was re-extracted with 1 mL of chloroform. Combined organic phases were dried under nitrogen. After dried extracts for plasma were resuspended in 100 µl Buffer B, 5 µL of sample was injected.

Combined analysis:

Analysis ID AN005565
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1260 Infinity II
Column Peeke Scientific Spectra C8SR (150 x 3.0 mm, 3μm)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6460 QQQ
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH004229
Instrument Name:Agilent 1260 Infinity II
Column Name:Peeke Scientific Spectra C8SR (150 x 3.0 mm, 3μm)
Column Temperature:40˚C
Flow Gradient:0 min, 82% B; 3 min, 82% B; 4 min, 90% B; 18 min, 99% B; 25 min, 99% B; 27 min, 82% B; 30 min, 82% B
Flow Rate:0.5 mL/min
Solvent A:100% water; 0.2% formic acid; 2 mM ammonium formate
Solvent B:100% methanol; 0.2% formic acid; 1 mM ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS005290
Analysis ID:AN005565
Instrument Name:Agilent 6460 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Sphingolipid species were analyzed by multiple reaction monitoring of the transition from precursor to product ions at associated optimized collision energies, and fragmentor voltages using Agilent Masshunter. The m/z values of the precursor and product ions are provided in the metabolite metadata section.
Ion Mode:POSITIVE
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