Summary of Study ST003393

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002102. The data can be accessed directly via it's Project DOI: 10.21228/M8X24G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003393
Study TitleFree fatty acid and lysophospholipids analysis of colon in Pla2g10 knockout mice.
Study SummaryGroup X Phospholipase A2 Links Colonic Lipid Homeostasis to Systemic Metabolism via Host-Microbiota Interaction - Free fatty acid and lysophospholipids analysis of colon in Pla2g10 knockout mice.
Institute
University of Tokyo
Last NameSato
First NameHiroyasu
Address7-3-1 Hongo, Bunkyo-ku Tokyo 113-8655, Japan.
Emailsato-hr@m.u-tokyo.ac.jp
Phone81-3-5841-1433
Submit Date2024-07-25
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-08-30
Release Version1
Hiroyasu Sato Hiroyasu Sato
https://dx.doi.org/10.21228/M8X24G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002102
Project DOI:doi: 10.21228/M8X24G
Project Title:Group X Phospholipase A2 Links Colonic Lipid Homeostasis to Systemic Metabolism via Host-Microbiota Interaction
Project Summary:The gut microbiota influences physiological functions of the host, ranging from the maintenance of local gut homeostasis to systemic immunity and metabolism. Secreted phospholipase A2 group X (sPLA2-X) is abundantly expressed in colonic epithelial cells but is barely detectable in metabolic and immune tissues. Despite this distribution, sPLA2-X-deficient (Pla2g10-/-) mice displayed variable obesity-related phenotypes that were abrogated after treatment with antibiotics or cohousing with Pla2g10+/+ mice, suggesting the involvement of gut microbiota. Under housing conditions where Pla2g10-/- mice showed aggravation of diet-induced obesity and insulin resistance, they displayed increased colonic inflammation and epithelial damage, reduced production of polyunsaturated fatty acids (PUFAs) and lysophospholipids, decreased abundance of several Clostridium species, and reduced levels of short-chain fatty acids (SCFAs). These obesity-related phenotypes in Pla2g10-/- mice were reversed by dietary supplementation with omega-3 PUFAs or SCFAs. Thus, colonic sPLA2-X orchestrates omega-3 PUFA-SCFA interplay via modulation of gut microbiota, thereby secondarily affecting systemic metabolism.
Institute:University of Tokyo
Last Name:Sato
First Name:Hiroyasu
Address:Tokyo 113-8655, Japan.
Email:sato-hr@m.u-tokyoac.jp
Phone:81-3-5841-1433

Subject:

Subject ID:SU003516
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Female

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Treatment Co-housing Sample source
SA368412HW_ 18Pla2g10+/+ High fat diet co-housed Colon
SA368413HW_139Pla2g10+/+ High fat diet co-housed Colon
SA368414HW_131Pla2g10+/+ High fat diet co-housed Colon
SA368415HW_128Pla2g10+/+ High fat diet co-housed Colon
SA368416HW_127Pla2g10+/+ High fat diet co-housed Colon
SA368417HW_ 23Pla2g10+/+ High fat diet co-housed Colon
SA368418HW_ 22Pla2g10+/+ High fat diet co-housed Colon
SA368454HK_ 20Pla2g10-/- High fat diet co-housed Colon
SA368455HK_ 138Pla2g10-/- High fat diet co-housed Colon
SA368456HK_ 134Pla2g10-/- High fat diet co-housed Colon
SA368457HK_ 133Pla2g10-/- High fat diet co-housed Colon
SA368458HK_ 132Pla2g10-/- High fat diet co-housed Colon
SA368459HK_ 23Pla2g10-/- High fat diet co-housed Colon
SA368460HK_ 22Pla2g10-/- High fat diet co-housed Colon
SA368397H_9Pla2g10+/+ High fat diet - Colon
SA368398HCt_1Pla2g10+/+ High fat diet - Colon
SA368399H_23Pla2g10+/+ High fat diet - Colon
SA368400H_22Pla2g10+/+ High fat diet - Colon
SA368401H_21Pla2g10+/+ High fat diet - Colon
SA368402H_20Pla2g10+/+ High fat diet - Colon
SA368403H_10Pla2g10+/+ High fat diet - Colon
SA368404HCt_2Pla2g10+/+ High fat diet - Colon
SA368405H_8Pla2g10+/+ High fat diet - Colon
SA368406H_7Pla2g10+/+ High fat diet - Colon
SA368407HCt_3Pla2g10+/+ High fat diet - Colon
SA368408HCt_13Pla2g10+/+ High fat diet - Colon
SA368409HCt_14Pla2g10+/+ High fat diet - Colon
SA368410HCt_15Pla2g10+/+ High fat diet - Colon
SA368411HCt_16Pla2g10+/+ High fat diet - Colon
SA368439HCt_9Pla2g10-/- High fat diet - Colon
SA368440HCt_23Pla2g10-/- High fat diet - Colon
SA368441HCt_21Pla2g10-/- High fat diet - Colon
SA368442HCt_22Pla2g10-/- High fat diet - Colon
SA368443HCt_24Pla2g10-/- High fat diet - Colon
SA368444HCt_25Pla2g10-/- High fat diet - Colon
SA368445HCt_7Pla2g10-/- High fat diet - Colon
SA368446H_32Pla2g10-/- High fat diet - Colon
SA368447H_12Pla2g10-/- High fat diet - Colon
SA368448H_31Pla2g10-/- High fat diet - Colon
SA368449H_30Pla2g10-/- High fat diet - Colon
SA368450H_11Pla2g10-/- High fat diet - Colon
SA368451H_29Pla2g10-/- High fat diet - Colon
SA368452H_14Pla2g10-/- High fat diet - Colon
SA368453H_13Pla2g10-/- High fat diet - Colon
SA368390HS_20Pla2g10+/+ High fat diet+SCFA - Colon
SA368391HS_19Pla2g10+/+ High fat diet+SCFA - Colon
SA368392HS_18Pla2g10+/+ High fat diet+SCFA - Colon
SA368393HS_17Pla2g10+/+ High fat diet+SCFA - Colon
SA368394HS_6Pla2g10+/+ High fat diet+SCFA - Colon
SA368395HS_5Pla2g10+/+ High fat diet+SCFA - Colon
SA368396HS_4Pla2g10+/+ High fat diet+SCFA - Colon
SA368432HS_27Pla2g10-/- High fat diet+SCFA - Colon
SA368433HS_26Pla2g10-/- High fat diet+SCFA - Colon
SA368434HS_28Pla2g10-/- High fat diet+SCFA - Colon
SA368435HS_10Pla2g10-/- High fat diet+SCFA - Colon
SA368436HS_29Pla2g10-/- High fat diet+SCFA - Colon
SA368437HS_11Pla2g10-/- High fat diet+SCFA - Colon
SA368438HS_12Pla2g10-/- High fat diet+SCFA - Colon
SA368427LW_ 141Pla2g10+/+ Low fat diet co-housed Colon
SA368428LW_114Pla2g10+/+ Low fat diet co-housed Colon
SA368429LW_140Pla2g10+/+ Low fat diet co-housed Colon
SA368430LW_138Pla2g10+/+ Low fat diet co-housed Colon
SA368431LW_119Pla2g10+/+ Low fat diet co-housed Colon
SA368468LK_146Pla2g10-/- Low fat diet co-housed Colon
SA368469LK_ 143Pla2g10-/- Low fat diet co-housed Colon
SA368470LK_122Pla2g10-/- Low fat diet co-housed Colon
SA368471LK_114Pla2g10-/- Low fat diet co-housed Colon
SA368472LK_ 112Pla2g10-/- Low fat diet co-housed Colon
SA368419L_1Pla2g10+/+ Low fat diet - Colon
SA368420L_19Pla2g10+/+ Low fat diet - Colon
SA368421L_17Pla2g10+/+ Low fat diet - Colon
SA368422L_16Pla2g10+/+ Low fat diet - Colon
SA368423L_15Pla2g10+/+ Low fat diet - Colon
SA368424L_3Pla2g10+/+ Low fat diet - Colon
SA368425L_2Pla2g10+/+ Low fat diet - Colon
SA368426L_18Pla2g10+/+ Low fat diet - Colon
SA368461L_26Pla2g10-/- Low fat diet - Colon
SA368462L_25Pla2g10-/- Low fat diet - Colon
SA368463L_4Pla2g10-/- Low fat diet - Colon
SA368464L_5Pla2g10-/- Low fat diet - Colon
SA368465L_28Pla2g10-/- Low fat diet - Colon
SA368466L_6Pla2g10-/- Low fat diet - Colon
SA368467L_27Pla2g10-/- Low fat diet - Colon
Showing results 1 to 83 of 83

Collection:

Collection ID:CO003509
Collection Summary:All mice groups underwent anesthesia with isoflurane. The mouse colons were excised and rinsed in ice-cold PBS. Colon was collected and was quickly frozen by using liquid nitrogen. Samples were preserved below -80 °C and were sent out for lipidomic analysis.
Sample Type:Colon

Treatment:

Treatment ID:TR003525
Treatment Summary:Pla2g10+/+ and Pla2g10-/- mice were subjected to high-fat diet (HFD) or low-fat diet (LFD) for 24 weeks. Additionally, co-housed mice were given same diets. Furthermore, for mice on HFD, the effects of adding short-chain fatty acids (SCFAs) to drinking water were compared with those without SCFAs.

Sample Preparation:

Sampleprep ID:SP003523
Sampleprep Summary:Tissues were soaked in methanol and homogenized with bead homogenizer (Bertin Instruments). Lipids were extracted from the homogenates by the method of Bligh and Dyer

Combined analysis:

Analysis ID AN005567
Analysis type MS
Chromatography type Reversed phase
Chromatography system Shimadzu Nexera X2
Column Phenomenex Kinetex C18 (50 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 4000 QTrap
Ion Mode NEGATIVE
Units Peak area/mg of wet tissue

Chromatography:

Chromatography ID:CH004231
Instrument Name:Shimadzu Nexera X2
Column Name:Phenomenex Kinetex C18 (50 x 2.1mm,1.7um)
Column Temperature:50
Flow Gradient:0–3 min 0% B; 3–6 min 0–60% B; 6–17 min 60–62.2% B; 17–17.01 min 62.2–69.8% B; 17.01–20 min 69.8–100% B; 20–23 min 100% B; 23–23.10 min 100–0% B; 23.10–26 min 0% B
Flow Rate:0.2 mL/min
Solvent A:33.3% acetonitrile/33.3% methanol/33.3% water; 5μM phosphoric acid; 1mM ammonium formate
Solvent B:100% isopropanol; 5μM phosphoric acid; 1mM ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS005292
Analysis ID:AN005567
Instrument Name:ABI Sciex 4000 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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