Summary of Study ST003394
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002102. The data can be accessed directly via it's Project DOI: 10.21228/M8X24G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003394 |
| Study Title | Fatty acid oxygenated metabolites analysis of colon in Pla2g10 knockout mice. |
| Study Summary | Group X Phospholipase A2 Links Colonic Lipid Homeostasis to Systemic Metabolism via Host-Microbiota Interaction. Analysis of fatty acid oxygenated metabolites in the colons of WT and Pla2g10 knockout mice that were fed either low-fat diet (LFD) or high-fat diet (HFD). |
| Institute | University of Tokyo |
| Last Name | Sato |
| First Name | Hiroyasu |
| Address | 7-3-1 Hongo, Bunkyo-ku Tokyo 113-8655, Japan. |
| sato-hr@m.u-tokyo.ac.jp | |
| Phone | 81-3-5841-1433 |
| Submit Date | 2024-07-31 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-08-30 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002102 |
| Project DOI: | doi: 10.21228/M8X24G |
| Project Title: | Group X Phospholipase A2 Links Colonic Lipid Homeostasis to Systemic Metabolism via Host-Microbiota Interaction |
| Project Summary: | The gut microbiota influences physiological functions of the host, ranging from the maintenance of local gut homeostasis to systemic immunity and metabolism. Secreted phospholipase A2 group X (sPLA2-X) is abundantly expressed in colonic epithelial cells but is barely detectable in metabolic and immune tissues. Despite this distribution, sPLA2-X-deficient (Pla2g10-/-) mice displayed variable obesity-related phenotypes that were abrogated after treatment with antibiotics or cohousing with Pla2g10+/+ mice, suggesting the involvement of gut microbiota. Under housing conditions where Pla2g10-/- mice showed aggravation of diet-induced obesity and insulin resistance, they displayed increased colonic inflammation and epithelial damage, reduced production of polyunsaturated fatty acids (PUFAs) and lysophospholipids, decreased abundance of several Clostridium species, and reduced levels of short-chain fatty acids (SCFAs). These obesity-related phenotypes in Pla2g10-/- mice were reversed by dietary supplementation with omega-3 PUFAs or SCFAs. Thus, colonic sPLA2-X orchestrates omega-3 PUFA-SCFA interplay via modulation of gut microbiota, thereby secondarily affecting systemic metabolism. |
| Institute: | University of Tokyo |
| Last Name: | Sato |
| First Name: | Hiroyasu |
| Address: | Tokyo 113-8655, Japan. |
| Email: | sato-hr@m.u-tokyoac.jp |
| Phone: | 81-3-5841-1433 |
Subject:
| Subject ID: | SU003517 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype | Treatment | Sample source |
|---|---|---|---|---|
| SA368473 | H_7 | Pla2g10+/+ | High fat diet | Colon |
| SA368474 | H_23 | Pla2g10+/+ | High fat diet | Colon |
| SA368475 | H_22 | Pla2g10+/+ | High fat diet | Colon |
| SA368476 | H_21 | Pla2g10+/+ | High fat diet | Colon |
| SA368477 | H_20 | Pla2g10+/+ | High fat diet | Colon |
| SA368478 | H_10 | Pla2g10+/+ | High fat diet | Colon |
| SA368479 | H_9 | Pla2g10+/+ | High fat diet | Colon |
| SA368480 | H_8 | Pla2g10+/+ | High fat diet | Colon |
| SA368489 | H_11 | Pla2g10-/- | High fat diet | Colon |
| SA368490 | H_12 | Pla2g10-/- | High fat diet | Colon |
| SA368491 | H_13 | Pla2g10-/- | High fat diet | Colon |
| SA368492 | H_14 | Pla2g10-/- | High fat diet | Colon |
| SA368493 | H_29 | Pla2g10-/- | High fat diet | Colon |
| SA368494 | H_30 | Pla2g10-/- | High fat diet | Colon |
| SA368495 | H_31 | Pla2g10-/- | High fat diet | Colon |
| SA368496 | H_32 | Pla2g10-/- | High fat diet | Colon |
| SA368481 | L_1 | Pla2g10+/+ | Low fat diet | Colon |
| SA368482 | L_19 | Pla2g10+/+ | Low fat diet | Colon |
| SA368483 | L_3 | Pla2g10+/+ | Low fat diet | Colon |
| SA368484 | L_15 | Pla2g10+/+ | Low fat diet | Colon |
| SA368485 | L_17 | Pla2g10+/+ | Low fat diet | Colon |
| SA368486 | L_18 | Pla2g10+/+ | Low fat diet | Colon |
| SA368487 | L_16 | Pla2g10+/+ | Low fat diet | Colon |
| SA368488 | L_2 | Pla2g10+/+ | Low fat diet | Colon |
| SA368497 | L_4 | Pla2g10-/- | Low fat diet | Colon |
| SA368498 | L_28 | Pla2g10-/- | Low fat diet | Colon |
| SA368499 | L_27 | Pla2g10-/- | Low fat diet | Colon |
| SA368500 | L_6 | Pla2g10-/- | Low fat diet | Colon |
| SA368501 | L_26 | Pla2g10-/- | Low fat diet | Colon |
| SA368502 | L_25 | Pla2g10-/- | Low fat diet | Colon |
| SA368503 | L_5 | Pla2g10-/- | Low fat diet | Colon |
| Showing results 1 to 31 of 31 |
Collection:
| Collection ID: | CO003510 |
| Collection Summary: | All mice groups underwent anesthesia with isoflurane. The mouse colons were excised and rinsed in ice-cold PBS. Colon was collected and was quickly frozen by using liquid nitrogen. Samples were preserved below -80 °C and were sent out for lipidomic analysis. |
| Sample Type: | Colon |
Treatment:
| Treatment ID: | TR003526 |
| Treatment Summary: | Pla2g10+/+ and Pla2g10-/- mice fed a high fat diet (HFD) or low fat diet (LFD) for 24 weeks. |
Sample Preparation:
| Sampleprep ID: | SP003524 |
| Sampleprep Summary: | Tissues were soaked in methanol and then homogenized with bead homogenizer. After overnight incubation at −20°C, water was added to the mixture to give a final methanol concentration of 10% (v/v). The samples in 10% methanol were applied to Oasis HLB cartridges (Waters), washed with 10 mL of hexane, eluted with 3 mL of methyl formate, dried under N2 gas, and dissolved in 60% methanol. |
Combined analysis:
| Analysis ID | AN005568 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu Nexera X2 |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 4500 Qtrap |
| Ion Mode | NEGATIVE |
| Units | Peak area/mg of wet tissue |
Chromatography:
| Chromatography ID: | CH004232 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40°C |
| Flow Gradient: | 0–2 min 90% B; 2–10 min 90-30% B; 10-24 min 30-27% B; 24-24.01 min 27-1% B; 24.01–27 min 1% B; 27–27.01 min 1-90% B; 27.01–32 min 90% B; 32-35 min 90-1% B; 35-39.97 min 1% B; 39.97-39.98 min 1-90% B; 39.98-42 min 90% B; 42-45 min 90% B |
| Flow Rate: | 0.3 ml/min |
| Solvent A: | 100% water; 10mM ammonium acetate |
| Solvent B: | 80% acetonitrile/20% methanol; 10mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS005293 |
| Analysis ID: | AN005568 |
| Instrument Name: | ABI Sciex 4500 Qtrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
