Summary of Study ST003519
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002164. The data can be accessed directly via it's Project DOI: 10.21228/M8WR6T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003519 |
| Study Title | Effect of insluin on metabolism of ex vivo ischemia heart |
| Study Summary | Ex vivo hearts were pre-perfused with or without insulin, followed by ischemia induced by the cessation of perfusion. After 25 minutes of ischemia, hearts underwent 30 minutes of reperfusion. Right atrial tissue was then collected for IC-MS analysis. Three groups were included: a control group without ischemia, a group with 25 minutes of ischemia followed by reperfusion, and a group pre-perfused with insulin for 30 minutes prior to ischemia and reperfusion. |
| Institute | Mayo Clinic |
| Last Name | Qiu |
| First Name | Huiliang |
| Address | 5951 E Mayo Blvd, RE 1-408, Zhu Lab, Phoenix, AZ 85054 |
| Qiu.Huiliang@mayo.edu | |
| Phone | 415-629-7041 |
| Submit Date | 2024-10-11 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-11-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002164 |
| Project DOI: | doi: 10.21228/M8WR6T |
| Project Title: | Insulin mitigates acute ischemia induced atrial fibrillation and sinoatrial node dysfunction ex vivo |
| Project Summary: | Acute atrial ischemia is a well-known cause of postoperative atrial fibrillation (POAF). However, the mechanisms through which ischemia contributes to the development of POAF are not well understood. In this study, ex vivo Langendorff perfusion was employed to induce acute atrial ischemia and reperfusion in the heart, resulting in a temporary form of POAF. The likelihood of atrial fibrillation (AF) was evaluated using programmed electrical stimulation and confirmed through open-atrium optical mapping. Compared to the control group without ischemia, 25 minutes of ischemia significantly increased the incidence of AF. The right atrium was found to be more susceptible to AF than the left atrium. Administering insulin for 30 minutes before ischemia and during reperfusion after 25 minutes of ischemia greatly reduced the vulnerability to AF. Additionally, IC-MS was utilized to assess the effect of insulin on the metabolism of the ischemic and reperfused heart. |
| Institute: | Mayo Clinic |
| Last Name: | Zhu |
| First Name: | Wuqiang |
| Address: | 5951 E Mayo Blvd, RE 1-408, Zhu Lab, Phoenix, AZ 85054 |
| Email: | zhu.wuqiang@mayo.edu |
| Phone: | 480-301-9693 |
Subject:
| Subject ID: | SU003648 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | WT |
| Gender: | Male and female |
Factors:
Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Treatment |
|---|---|---|---|
| SA386506 | IS25_A | Right atrial tissue | "Ischemia for 25 mins, followed by reperfusion for 30 mins" |
| SA386507 | IS25_B | Right atrial tissue | "Ischemia for 25 mins, followed by reperfusion for 30 mins" |
| SA386508 | IS25_C | Right atrial tissue | "Ischemia for 25 mins, followed by reperfusion for 30 mins" |
| SA386509 | N1_R | Right atrial tissue | Non-ischemia control |
| SA386510 | N2_R | Right atrial tissue | Non-ischemia control |
| SA386511 | N3_R | Right atrial tissue | Non-ischemia control |
| SA386512 | N4_R | Right atrial tissue | Non-ischemia control |
| SA386513 | IS25_INS_A | Right atrial tissue | Preperfusion of insluin for 30 mins before ischemia and reperfusion |
| SA386514 | IS25_INS_B | Right atrial tissue | Preperfusion of insluin for 30 mins before ischemia and reperfusion |
| SA386515 | IS25_INS_C | Right atrial tissue | Preperfusion of insluin for 30 mins before ischemia and reperfusion |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO003641 |
| Collection Summary: | Rat hearts were isolated for ex vivo Langendorff perfusion. After the treatments were applied, right atrial tissue was collected and frozen at -80°C until IC-MS analysis |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR003657 |
| Treatment Summary: | Ex vivo hearts were pre-perfused with or without insulin, followed by ischemia induced by the cessation of perfusion. After 25 minutes of ischemia, hearts underwent 30 minutes of reperfusion. Right atrial tissue was then collected for IC-MS analysis |
Sample Preparation:
| Sampleprep ID: | SP003655 |
| Sampleprep Summary: | Tissue were snap frozen in liquid nitrogen, then homogenized with Precellys Tissue Homogenizer. Certain volumes of homogenate were used to make sure same amount of tissue were extracted. Metabolites were extracted using ice-cold 80/20 (v/v) methanol/water with 0.1% ammonium hydroxide. Extracts were vortexed vigorously for 2 mins, centrifuged at 17,000 g for 10 min at 4°C, and supernatants were transferred to clean tubes, followed by evaporation to dryness under nitrogen. Dried extracts were reconstituted in 100 μL of 1mM potassium hydroxide (KOH) in deionized water. |
Combined analysis:
| Analysis ID | AN005777 |
|---|---|
| Analysis type | MS |
| Chromatography type | Ion exchange |
| Chromatography system | Thermo Dionex ICS-6000+ |
| Column | Thermo IonPac AS11 (250 x 2 mm, 4 μm) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Ortibrap IQ-X |
| Ion Mode | NEGATIVE |
| Units | Area under the Curve |
Chromatography:
| Chromatography ID: | CH004384 |
| Chromatography Summary: | ion chromatography with high resolution mass spectrometry |
| Instrument Name: | Thermo Dionex ICS-6000+ |
| Column Name: | Thermo IonPac AS11 (250 x 2 mm, 4 μm) |
| Column Temperature: | 35°C |
| Flow Gradient: | 0-5 min, 1% MPB; 5-25 min, 1-35% MPB; 25-39 min, 35-99% MPB; 39-49 min, 99% MPB; 49-50 min, 99-1% MPB |
| Flow Rate: | 360 μL/min |
| Solvent A: | 100% Water |
| Solvent B: | 100% Water; 100 mM KOH (potassium hydroxide) |
| Chromatography Type: | Ion exchange |
MS:
| MS ID: | MS005497 |
| Analysis ID: | AN005777 |
| Instrument Name: | Thermo Ortibrap IQ-X |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data were acquired using a Thermo Orbitrap IQ-X Tribrid Mass Spectrometer under ESI negative ionization. All the raw data files were imported into Thermo Trace Finder 5.1 software for final analysis. |
| Ion Mode: | NEGATIVE |
