Summary of Study ST003632

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002245. The data can be accessed directly via it's Project DOI: 10.21228/M8F83T This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003632
Study TitleDysregulated Lipid Metabolism in African American Women Who Experienced Cardiometabolic Complications of Pregnancy
Study SummaryPatients were recruited from Grady Memorial and Emory University Midtown. Patients who agreed to take part in the study consented to blood draw at 8-10 months postpartum. For this study, women who had a diagnosis of gestational diabetes (n = 3), gestational hypertension (n = 5), or preeclampsia (n = 2), were analyzed together as the cardiometabolic complications (CMCPreg) group and those whose pregnancy resulted in a full-term birth without cardiometabolic complications of pregnancy were regarded as the healthy control group (n = 22). Patient metadata and lipidomics data was integrated for analysis. We identified 11 lipids in the sphingolipid, glycerolipid, and cholesteryl ester lipid classes that are dysregulated at 8-10 months postpartum that can potentially be leveraged as biomarkers to prevent future disease diagnoses.
Institute
Emory University
Last NameManer-Smith
First NameKristal
AddressEIMLC/1750 Haygood Dr./Office N256/ Atlanta, GA 30322
Emailkmaners@emory.edu
Phone4047273193
Submit Date2024-11-25
Num Groups2
Total Subjects31
Num Females31
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2025-11-26
Release Version1
Kristal Maner-Smith Kristal Maner-Smith
https://dx.doi.org/10.21228/M8F83T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002245
Project DOI:doi: 10.21228/M8F83T
Project Title:Untargeted Lipidomics of African American women at 8-10 months postpartum
Project Type:Untargeted Lipidomics with DDA
Project Summary:Lipid dysregulation is hallmark of CMCPreg, specifically polyunsaturated fatty acids (PUFA) metabolism and downstream lipid mediators. To investigate whether this lipid dysregulation is prolonged after delivery, we performed untargeted lipidomics on serum collected 8-10 months postpartum from African American women who were diagnosed with cardiometabolic complications of pregnancy and from those with uncomplicated, term deliveries. We identified 11 lipids that were significantly increased in the postpartum serum of African American women who experienced cardiometabolic complications of pregnancy. These species belonged to sphingolipid, glycerolipid, and cholesteryl ester lipid classes and reveal dysregulated activity of glycerophospholipid metabolism, sphingolipid metabolism, sphingolipid signaling pathways, ferroptosis, and others by lipid enrichment pathway analysis. We also observed increases in Resolvin D3, 13 HpODE, AEA, OEA, and LEA suggesting dysregulated endocannabinoid signaling and increased phospholipaseA2 activity. Combined, these findings demonstrate that African American women who experienced cardiometabolic complications of pregnancy may have dysregulated lipid metabolism that extends as long as 8-10 months postpartum.
Institute:Emory University
Last Name:Maner-Smith
First Name:Kristal
Address:1750 Haygood Dr. Office N256 Atlanta, GA 30322
Email:kmaners@emory.edu
Phone:4047273193
Funding Source:NIH
Publications:PMID 38668319

Subject:

Subject ID:SU003762
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female
Human Ethnicity:African American

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA393698G0108_PPControl
SA393699G0301_PPControl
SA393700G0294_PPControl
SA393701G0291_PPControl
SA393702G0277_PPControl
SA393703G0259_PPControl
SA393704G0251_PPControl
SA393705G0170_PPControl
SA393706G0128_PPControl
SA393707E0588_PPControl
SA393708G0122_PPControl
SA393709E0555_PPControl
SA393710E0641_PPControl
SA393711E0711_PPControl
SA393712E0680_PPControl
SA393713E0592_PPControl
SA393714E0650_PPControl
SA393715E0606_PPControl
SA393716E0609_PPControl
SA393717E0648_PPControl
SA393718E0619_PPControl
SA393719E0627_PPControl
SA393720G0105_PPRisk
SA393721G0195_PPRisk
SA393722G0210_PPRisk
SA393723G0204_PPRisk
SA393724G0084_PPRisk
SA393725G0183_PPRisk
SA393726G0138_PPRisk
SA393727G0130_PPRisk
SA393728G0125_PPRisk
SA393729G0124_PPRisk
Showing results 1 to 32 of 32

Collection:

Collection ID:CO003755
Collection Summary:Study participants consented to blood draw at 8-10 months postpartum. Blood was collected, serum prepared, and stored in a -80 freezer until lipidomics was performed.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR003771
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP003769
Sampleprep Summary:Briefly, 50 µL serum was extracted using MtBE and methanol using an automated sample preparation system. Briefly, samples were spiked with 10 uL deuterated analytical standard, Splash Lipidomix (Avanti Polar, Alabaster, AL) and 200 uL methanol containing 50µg/mL BHT. Samples were vortexed, centrifuged at 1000xg for 3 minutes, and filtered using a 0.25 um polytetrafluoroethylene (PFTE) filter. To the supernatant, 250 µL MtBE:methanol (3:1 v/v) was added and the resulting samples were then mixed, centrifuged, and filtered as previously. The recovered extract was then dried under nitrogen gas and subsequently reconsitituted to 200 uL in acetonitrile:isopropanol (1:1 v/v) for LC/MS/MS analysis.

Chromatography:

Chromatography ID:CH004533
Chromatography Summary:Time (minutes) A: 10mM ammonium formate in 60% ACN w/ 0.1% FA B: 10mM ammonium formate in 10:90% ACN:IPA w/ 0.1% FA Time %A %B 0 60 40 0.2 60 40 1.5 40 60 6 30 70 9 15 85 11 0 100 12.5 0 100 13 60 40 15 60 40
Instrument Name:Thermo Vanquish
Column Name:Thermo Accucore C18 (100 x 4.6mm, 2.6um)
Column Temperature:50
Flow Gradient:Linear: Time %A %B; 0 60 40; 0.2 60 40; 1.5 40 60; 6 30 70; 9 15 85; 11 0 100; 12.5 0 100; 13 60 40; 15 60 40
Flow Rate:0.5 mL/min
Solvent A:60% Acetonitrile/40% Water; 10mM Ammonium Formate; 0.1% Formic Acid
Solvent B:90% Isopropanol/10% Acetonitrile; 10mM Ammonium Formate; 0.1% Formic Acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN005965
Analysis Type:MS
Chromatography ID:CH004533
Num Factors:2
Num Metabolites:1866
Units:Normalized Peak Area
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