Summary of Study ST004125

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002593. The data can be accessed directly via it's Project DOI: 10.21228/M8GG2T This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST004125
Study TitleMetabolite profiling in the glycerol kinase (GK), GPD1 and GAPDH genes into the reconstituted ChREBPα-MLX HEK293T system alongside green fluorescent protein (GFP).
Study SummaryTo distinguish between potential ChREBP-activating ligands, we introduced glycerol kinase (GK), GPD1 and GAPDH genes into the reconstituted ChREBP MLX HEK293T system alongside green fluorescent protein (GFP).Relative quantification of 137 metabolites showed that in these cells, GPD1 strongly depressed levels of GA3P and DHAP, while elevating G3P.
Institute
University of Chicago
Last NameShah
First NameHardik
Address900 E 57th street, Chicago IL 60637. USA.
Emailhardikshah@uchicago.edu
Phone7738348830
Submit Date2025-08-13
Num Groups2
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2025-08-22
Release Version1
Hardik Shah Hardik Shah
https://dx.doi.org/10.21228/M8GG2T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002593
Project DOI:doi: 10.21228/M8GG2T
Project Title:Glycerol-3-phosphate activates ChREBP, FGF21 transcription and lipogenesis 2 in Citrin Deficiency
Project Summary:Citrin Deficiency (CD) is caused by inactivation of SLC25A13, a mitochondrial membrane protein required to move electrons from cytosolic NADH to the mitochondrial matrix in hepatocytes. People with CD do not like sweets. We discovered that SLC25A13 loss causes accumulation of glycerol-3-phosphate (G3P), which activates carbohydrate response element binding protein (ChREBP) to transcribe FGF21, which acts in the brain to restrain intake of sweets and alcohol, and to transcribe key genes driving lipogenesis. Mouse and human data establish G3P-ChREBP as a new mechanistic component of the Randle Cycle that contributes to metabolic dysfunction-associated steatotic liver disease (MASLD) and forms part of a system that communicates metabolic states from liver to brain in a manner that alters food and alcohol choices. The data provide a framework for understanding FGF21 induction in varied conditions, suggest ways to develop FGF21-inducing drugs, and drug candidates for lean MASLD and support of urea cycle function in CD.
Institute:University of Chicago
Laboratory:UCCC-Metabolomics Platform
Last Name:Shah
First Name:Hardik
Address:900 E 57th street, Chicago IL 60637. USA.
Email:hardikshah@uchicago.edu
Phone:7738348830

Subject:

Subject ID:SU004274
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Genotype
SA476485GAPDH_11HEK293T GAPDH
SA476486GAPDH_10HEK293T GAPDH
SA476487GAPDH_12HEK293T GAPDH
SA476494GPD1_07HEK293T Genotype
SA476495GPD1_08HEK293T Genotype
SA476496GPD1_09HEK293T Genotype
SA476488GFP_01HEK293T GFP
SA476489GFP_02HEK293T GFP
SA476490GFP_03HEK293T GFP
SA476491GK_04HEK293T GK
SA476492GK_05HEK293T GK
SA476493GK_06HEK293T GK
Showing results 1 to 12 of 12

Collection:

Collection ID:CO004267
Collection Summary:48 hours after transfection with the specified plasmid, cells were washed with room-temperature PBS, immediately quenched with dry-ice-cold 80% methanol
Sample Type:Human embryonic kidney cells
Storage Conditions:-80℃

Treatment:

Treatment ID:TR004283
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP004280
Sampleprep Summary:Cells were washed with room-temperature PBS, immediately quenched with dry-ice-cold 80% methanol.Samples were centrifuged at 20,000 g for 20 minutes at 4°C, the supernatant was dried down on a Genvevac EZ-2 4.0 elite evaporator, and the samples were resuspended in 100 µL of 60/40 acetonitrile-water.

Chromatography:

Chromatography ID:CH005194
Chromatography Summary:The chromatography separation was performed using Thermo Scientific Vanquish Horizon UHPLC system and iHILIC-(P) Classic (2.1 x 150 mm, 5 µm; part # 160.152.0520; HILICON AB) column. The mobile phase A(MPA) was 20 mM ammonium bicarbonate at pH 9.6, adjusted by ammonium hydroxide addition and MPB was acetonitrile. The column temperature, injection volume, and the flow rate were 40°C, 2 µL, and 0.2 mL/minute, respectively. The chromatographic gradient was 0 minutes: 85% B, 0.5 minutes: 85% B, 18 minutes: 20% B, 20 minutes: 20% B, 20.5 minutes: 85% B and 28 minutes: 85% B.
Instrument Name:Thermo Vanquish
Column Name:HILICON iHILIC-(P) Classic (150 x 2.1 mm, 5 µm)
Column Temperature:40°C
Flow Gradient:The chromatographic gradient was 0 minutes: 85% B, 0.5 minutes: 85% B, 18 minutes: 20% B, 20 minutes: 20% B, 20.5 minutes: 85% B and 28 minutes: 85% B
Flow Rate:0.2 mL/min
Solvent A:100% Water; 20 mM ammonium bicarbonate; ammonium hydroxide 0.2%
Solvent B:100% Acetonitrile
Chromatography Type:HILIC

Analysis:

Analysis ID:AN006838
Analysis Type:MS
Chromatography ID:CH005194
Num Factors:4
Num Metabolites:137
Units:A.U.
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