Summary of Study ST004216
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002658. The data can be accessed directly via it's Project DOI: 10.21228/M82V85 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST004216 |
| Study Title | Paracrine stimulation of brown adipose tissue vascularization by adipocyte O-GlcNAc signaling |
| Study Summary | Brown adipose tissue (BAT) is extensively vascularized, which is essential for its physiological activities. The molecular mechanism that underpins BAT vascularization, however, remains poorly understood. This study presents evidence that acute cold exposure induces an elevation in total protein O-linked β-N-acetylglucosamine modification (O-GlcNAcylation) in BAT. Ablation of O-GlcNAc transferase in brown adipocytes drastically impairs BAT vascularization. Mechanistic studies demonstrate that OGlcNAcylation of specificity protein 1 (SP1) in brown adipocytes enhances its transcriptional activity towards kielin/chordin-like protein (Kcp). Secreted KCP subsequently promotes BAT angiogenesis by paracrine activation of BMP signaling. Furthermore, boosting O-GlcNAc signaling with glucosamine supplementation effectively augments BAT vascularization and thermogenesis. These findings thus uncover a previously unrecognized role of adipocyte O-GlcNAc signaling in the metabolism-driven regulation of BAT vascularization and function. |
| Institute | Shandong University |
| Last Name | Li |
| First Name | Luwen |
| Address | Shandong University Baotuquan Campus, Baotuquan Subdistrict, Lixia District, Jinan City, Shandong Province, China, JiNan, Shandong, 250012, China |
| liluwenchina@outlook.com | |
| Phone | 18766179319 |
| Submit Date | 2025-09-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002658 |
| Project DOI: | doi: 10.21228/M82V85 |
| Project Title: | Paracrine stimulation of brown adipose tissue vascularization by adipocyte O-GlcNAc signaling |
| Project Summary: | Brown adipose tissue (BAT) is extensively vascularized, which is essential for its physiological activities. The molecular mechanism that underpins BAT vascularization, however, remains poorly understood. This study presents evidence that acute cold exposure induces an elevation in total protein O-linked β-N-acetylglucosamine modification (O-GlcNAcylation) in BAT. Ablation of O-GlcNAc transferase in brown adipocytes drastically impairs BAT vascularization. Mechanistic studies demonstrate that O-GlcNAcylation of specificity protein 1 (SP1) in brown adipocytes enhances its transcriptional activity towards kielin/chordin-like protein (Kcp). Secreted KCP subsequently promotes BAT angiogenesis by paracrine activation of BMP signaling. Furthermore, boosting O-GlcNAc signaling with glucosamine supplementation effectively augments BAT vascularization and thermogenesis. These findings thus uncover a previously unrecognized role of adipocyte O-GlcNAc signaling in the metabolism-driven regulation of BAT vascularization and function. |
| Institute: | Shandong University |
| Last Name: | Li |
| First Name: | Luwen |
| Address: | Shandong University Baotuquan Campus, Baotuquan Subdistrict, Lixia District, Jinan City, Shandong Province, China, JiNan, Shandong, 250012, China |
| Email: | liluwenchina@outlook.com |
| Phone: | 18766179319 |
Subject:
| Subject ID: | SU004368 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype | Sample source |
|---|---|---|---|
| SA485755 | 23P10760001 | Wild-type | brown adipose tissue |
| SA485756 | 23P10760002 | Wild-type | brown adipose tissue |
| SA485757 | 23P10760003 | Wild-type | brown adipose tissue |
| SA485758 | 23P10760004 | Wild-type | brown adipose tissue |
| SA485759 | 23P10760005 | Wild-type | brown adipose tissue |
| SA485760 | 23P10760006 | Wild-type | brown adipose tissue |
| SA485761 | 23P10760007 | Wild-type | brown adipose tissue |
| SA485762 | 23P10760008 | Wild-type | brown adipose tissue |
| Showing results 1 to 8 of 8 |
Collection:
| Collection ID: | CO004361 |
| Collection Summary: | The samples were obtained from brown adipose tissue of C57 mice. Brown adipose tissue was harvested from the mice, with surrounding white adipose tissue removed. The tissue was stored in liquid nitrogen for subsequent analysis and testing. |
| Sample Type: | Brown adipose |
Treatment:
| Treatment ID: | TR004377 |
| Treatment Summary: | The mice were exposed to 4°C for cold stimulation over a period of two days, with sufficient food and water provided throughout the experiment. The control group mice were maintained at 23°C, with all other housing conditions consistent with the cold-stimulated group. Brown adipose tissue was collected from all mice after two days. |
Sample Preparation:
| Sampleprep ID: | SP004374 |
| Sampleprep Summary: | Sample Collection & Prep: Mouse brown fat was dissected on ice, cleaned of white fat, blotted dry, snap-frozen in liquid nitrogen, and stored at -80°C. Metabolite Extraction: Tissue was homogenized in a methanol-acetonitrile-water solution, settled, centrifuged, and freeze-dried. The dried metabolites were then dissolved in 50% methanol. Analysis: Metabolites were separated and analyzed using a UPLC system coupled to a high-resolution mass spectrometer. Data Processing: The data was analyzed using Compound Discoverer software with searches against multiple metabolomic databases (BMDB, mzCloud, ChemSpider). |
Combined analysis:
| Analysis ID | AN007014 | AN007015 |
|---|---|---|
| Chromatography ID | CH005324 | CH005325 |
| MS ID | MS006711 | MS006712 |
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters 2777C | Waters 2777C |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1 mm, 1.7 μm) | Waters ACQUITY UPLC CSH C18 (100 x 2.1 mm, 1.7 μm) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH005324 |
| Instrument Name: | Waters 2777C |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1 mm, 1.7 μm) |
| Column Temperature: | 45°C |
| Flow Gradient: | 0 - 1 min: 2% B 1 - 9 min: 2% to 98% B 9 - 12 min: 98% B 12 - 12.1 min: 98% to 2% B 12.1 - 15 min: 2% B |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% Water; 0.1% formic acid |
| Solvent B: | 100% Methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH005325 |
| Instrument Name: | Waters 2777C |
| Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 2.1 mm, 1.7 μm) |
| Column Temperature: | 45°C |
| Flow Gradient: | 0 - 1 min: 2% B 1 - 9 min: 2% to 98% B 9 - 12 min: 98% B 12 - 12.1 min: 98% to 2% B 12.1 - 15 min: 2% B |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% Water; 10 mM ammonium formate |
| Solvent B: | 95% Methanol/5% water; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS006711 |
| Analysis ID: | AN007014 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | ata-dependent acquisition (DDA) of both MS¹ and MS² spectra was performed using a Q Exactive HF mass spectrometer (Thermo Fisher Scientific, USA). The full-scan mass range was set to m/z 70–1050. MS¹ spectra were acquired at a resolution of 120,000 with an AGC target of 3e6 and a maximum injection time (IT) of 100 ms. Based on precursor intensity, the top 3 ions were selected for fragmentation. MS² spectra were acquired at a resolution of 30,000 with an AGC target of 1e5 and a maximum IT of 50 ms. Stepped normalized collision energy (stepped NCE) was set to 20, 40, and 60 eV.Data analysis was conducted using the Compound Discoverer 3.3 software (Thermo Fisher) in conjunction with the BMDB (UW Metabolome Database, BGI Metabolome Database), mzCloud database, and ChemSpider online database |
| Ion Mode: | POSITIVE |
| MS ID: | MS006712 |
| Analysis ID: | AN007015 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | ata-dependent acquisition (DDA) of both MS¹ and MS² spectra was performed using a Q Exactive HF mass spectrometer (Thermo Fisher Scientific, USA). The full-scan mass range was set to m/z 70–1050. MS¹ spectra were acquired at a resolution of 120,000 with an AGC target of 3e6 and a maximum injection time (IT) of 100 ms. Based on precursor intensity, the top 3 ions were selected for fragmentation. MS² spectra were acquired at a resolution of 30,000 with an AGC target of 1e5 and a maximum IT of 50 ms. Stepped normalized collision energy (stepped NCE) was set to 20, 40, and 60 eV.Data analysis was conducted using the Compound Discoverer 3.3 software (Thermo Fisher) in conjunction with the BMDB (UW Metabolome Database, BGI Metabolome Database), mzCloud database, and ChemSpider online database |
| Ion Mode: | NEGATIVE |
