Summary of Study ST004243

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002675. The data can be accessed directly via it's Project DOI: 10.21228/M8W83V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST004243
Study Title	Glycolysis inhibition functionally reprograms T follicular helper cells and reverses lupus - human Lupus patients
Study Summary	To elucidate cellular metabolic function changes, Tfh cells were purified from the blood of human Systemic lupus erythematosus (SLE) patients versus healthy controls. The metabolomes of Tfh cells were analyzed by LC-MS. The conclusion of this study is that autoimmune Tfh cells exhibit reduced mitochondrial metabolism when compared to healthy controls.
Institute	UT Health San Antonio
Department	Microbiology, Immunology. Molecular Genetics
Last Name	Yong
First Name	Ge
Address	7703 Flyod Curl Dr
Email	geyong725@gmail.com
Phone	210-567-3925
Submit Date	2025-09-25
Num Groups	2
Total Subjects	6
Publications	Glycolysis inhibition functionally reprograms T follicular helper cells and reverses lupus
Raw Data Available	Yes
Raw Data File Type(s)	mzXML
Analysis Type Detail	LC-MS
Release Date	2025-10-02
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR002675
Project DOI:	doi: 10.21228/M8W83V
Project Title:	Glycolysis inhibition functionally reprograms T follicular helper cells and reverses lupus
Project Summary:	This study aimed to understand the impact of 2-deoxy-D-glucose (2DG) in modulating the transcriptional and metabolomic responses of T follicular helper cells (Tfh) in a lupus-prone mouse model (W.Yaa). Thus, we analyzed the metabolomes of Tfh cells purified the spleen of W.Yaa mice treated with 2DG versus untreated controls. Data demonstrated that inhibition of glycolysis via 2DG treatment profoundly reprogrammed the metabolic pathways of splenic Tfh cells. This includes the suppression of the pentose phosphate pathway, essential for autoimmune Tfh cell expansion and the activation of mitochondrial metabolism. The reprogrammed metabolism of Tfh cells was associated with the reduction of autoantibodies and autoimmune phenotype.
Institute:	UT Health San Antonio
Department:	Microbiology, Immunology. Molecular Genetics
Last Name:	Yong
First Name:	Ge
Address:	7703 Flyod Curl Dr
Email:	geyong725@gmail.com
Phone:	210-567-3925
Funding Source:	NIH
Publications:	Glycolysis inhibition functionally reprograms T follicular helper cells and reverses lupus

Subject:

Subject ID:	SU004395
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample source	Group
SA490118	Tfh-HC-1	blood Tfh cells	HC
SA490119	Tfh-HC-2	blood Tfh cells	HC
SA490120	Tfh-HC-3	blood Tfh cells	HC
SA490121	Tfh-SLE-1	blood Tfh cells	SLE
SA490122	Tfh-SLE-2	blood Tfh cells	SLE
SA490123	Tfh-SLE-3	blood Tfh cells	SLE

Showing results 1 to 6 of 6

Showing results 1 to 6 of 6

Collection:

Collection ID:	CO004388
Collection Summary:	Tfh cells were purified from the peripheral blood of SLE patients and healthy controls (HCs) using flow cytometry. 2 groups of Tfh cells (SLE vs. HC) were purified and analyzed.
Sample Type:	Blood Tfh cells

Treatment:

Treatment ID:	TR004404
Treatment Summary:	Peripheral blood was collected from human SLE patients and healthy controls (HCs). Each groups had 3 subjects. Tfh cells were FACS-purified to perform untargeted metabolomics analysis using LC-MS.

Sample Preparation:

Sampleprep ID:	SP004401
Sampleprep Summary:	Global metabolomics profiling was performed on a Thermo Q-Exactive Oribtrap mass spectrometer with Dionex UHPLC and autosampler. Samples were homogenized in 5 mM ammonium acetate at 20 mg/ml. After homogenization, the sample was centrifuged at 20,000 rcf and 100 l was transferred to a new tube. Next, 800 μL of a mixture of acetonitrile, methanol, and acetone (8:1:1) was added followed by centrifugation to precipitate and pellet the proteins. The supernatant was transferred to a clean tube and dried under a gentle stream of nitrogen before reconstitution in 100 μL of 0.1% formic acid in water for metabolomic analysis.

Sampleprep ID:

SP004401

Sampleprep Summary:

Global metabolomics profiling was performed on a Thermo Q-Exactive Oribtrap mass spectrometer with Dionex UHPLC and autosampler. Samples were homogenized in 5 mM ammonium acetate at 20 mg/ml. After homogenization, the sample was centrifuged at 20,000 rcf and 100 l was transferred to a new tube. Next, 800 μL of a mixture of acetonitrile, methanol, and acetone (8:1:1) was added followed by centrifugation to precipitate and pellet the proteins. The supernatant was transferred to a clean tube and dried under a gentle stream of nitrogen before reconstitution in 100 μL of 0.1% formic acid in water for metabolomic analysis.

Chromatography:

Chromatography ID:	CH005363
Chromatography Summary:	Separation was achieved on an ACE 18-pfp 100 x 2.1 mm, 2 µm column with mobile phase A as 0.1% formic acid in water and mobile phase B as acetonitrile. This is a polar embedded stationary phase that provides comprehensive coverage, but does have some limitation is the coverage of very polar species. The flow rate was 350 µL/min with a column temperature of 25°C. 4 µL was injected for negative ions and 2 µL for positive ions.
Instrument Name:	Thermo Dionex
Column Name:	ACE C18-PFP (100 x 2.1 mm, 2 um)
Column Temperature:	25°C
Flow Gradient:	0-3 min: 0% B; 3-13min: 0% B - 80% B; 13-16 min: 80% B; 16-16.5 min: 80% B - 0% B; 16.5-20.5 min: 0% B
Flow Rate:	350 µL/min
Solvent A:	100% Water; 0.1% formic acid
Solvent B:	100% acetonitrile
Chromatography Type:	Reversed phase

Analysis:

Analysis ID:	AN007063
Analysis Type:	MS
Chromatography ID:	CH005363
Num Factors:	2
Num Metabolites:	154
Has Mz:	1
Has Rt:	1
Rt Units:	Minutes
Results File:	ST004243_AN007063_Results.txt
Units:	TIC-normalized intensity

Analysis ID:	AN007064
Analysis Type:	MS
Chromatography ID:	CH005363
Num Factors:	2
Num Metabolites:	175
Has Mz:	1
Has Rt:	1
Rt Units:	Minutes
Results File:	ST004243_AN007064_Results.txt
Units:	TIC-normalized intensity