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MGP000526

UniProt Annotations

Entry Information
Gene Namecentromere protein E, 312kDa
Protein EntryCENPE_HUMAN
UniProt IDQ02224
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q02224-1; Sequence=Displayed; Name=2; IsoId=Q02224-2; Sequence=VSP_028822; Name=3; IsoId=Q02224-3; Sequence=VSP_028820, VSP_028821;
DomainThe protein is composed of a N-terminal kinesin-motor domain involved in the chromosome movements, a long coil-coiled region involved in the homodimerization and an inhibitory C-tail involved in autoinhibition of the N-terminal catalytic part. {ECO:0000250}.
FunctionEssential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. {ECO:0000269|PubMed:17535814, ECO:0000269|PubMed:7889940}.
InteractionO60566:BUB1B; NbExp=4; IntAct=EBI-1375040, EBI-1001438; Q9BZD4:NUF2; NbExp=9; IntAct=EBI-1375040, EBI-724102;
PtmSumoylated with SUMO2 and SUMO3. The sumoylation mediates the association to the kinetochore. {ECO:0000269|PubMed:18374647}.
PtmThe C-terminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor (By similarity). {ECO:0000250}.
Sequence CautionSequence=BAE06078.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
SimilarityBelongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. {ECO:0000255|PROSITE- ProRule:PRU00283}.
SimilarityContains 1 kinesin motor domain. {ECO:0000255|PROSITE- ProRule:PRU00283}.
Subcellular LocationChromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.
SubunitMonomer. Interacts with CENPF, SEPT7, KIF18A, BUB1B kinase and PRC1. Interacts with NUF2; this interaction determines kinetochore localization. Interacts with SKAP; this interaction greatly favors SKAP binding to microtubules. {ECO:0000269|PubMed:15236970, ECO:0000269|PubMed:15297875, ECO:0000269|PubMed:17535814, ECO:0000269|PubMed:18460473, ECO:0000269|PubMed:19625775, ECO:0000269|PubMed:22110139, ECO:0000269|PubMed:9763420}.
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