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MGP002023

UniProt Annotations

Entry Information
Gene Namemyosin X
Protein EntryMYO10_HUMAN
UniProt IDQ9HD67
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative promoter usage, Alternative splicing; Named isoforms=3; Name=1; IsoId=Q9HD67-1; Sequence=Displayed; Name=2; IsoId=Q9HD67-2; Sequence=VSP_054976, VSP_054977; Note=No experimental confirmation available.; Name=Headless; IsoId=Q9HD67-3; Sequence=VSP_054975; Note=Produced by alternative promoter usage.;
CautionRepresents an unconventional myosin. This protein should not be confused with the conventional myosin-10 (MYH10). {ECO:0000305}.
DomainInteraction between the motor domain and the tail leads to an inactive, monomeric conformation. Phospholipid binding via the PH domains leads to the formation of the active, dimeric form of the protein and strongly increases actin-dependent ATPase activity and motor activity (By similarity). {ECO:0000250}.
DomainInteracts with membranes containing phosphatidylinositol- 3,4,5-trisphosphate via the PH domains. {ECO:0000250}.
DomainIQ 3 domain mediates high-affinity calcium-dependent binding to CALM3/CLP.
FunctionIsoform Headless: Functions as a dominant-negative regulator of isoform 1, suppressing its filopodia-inducing and axon outgrowth-promoting activities. In hippocampal neurons, it increases VASP retention in spine heads to induce spine formation and spine head expansion (By similarity). {ECO:0000250}.
FunctionMyosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as plus end-directed motor. The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate or integrins, and mediates cargo transport along actin filaments. Regulates cell shape, cell spreading and cell adhesion. Stimulates the formation and elongation of filopodia. May play a role in neurite outgrowth and axon guidance. In hippocampal neurons it induces the formation of dendritic filopodia by trafficking the actin-remodeling protein VASP to the tips of filopodia, where it promotes actin elongation. Plays a role in formation of the podosome belt in osteoclasts. {ECO:0000269|PubMed:16894163, ECO:0000269|PubMed:18570893}.
InteractionP62158:CALM3; NbExp=2; IntAct=EBI-307061, EBI-397435; P27482:CALML3; NbExp=2; IntAct=EBI-307061, EBI-747537; P43146:DCC; NbExp=7; IntAct=EBI-307061, EBI-1222919; P18084:ITGB5; NbExp=2; IntAct=EBI-307061, EBI-1223434;
PtmThe initiator methionine for isoform Headless is removed. {ECO:0000250}.
Sequence CautionSequence=BAA34519.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
SimilarityBelongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. {ECO:0000305}.
SimilarityContains 1 FERM domain. {ECO:0000255|PROSITE- ProRule:PRU00084}.
SimilarityContains 1 myosin motor domain. {ECO:0000305}.
SimilarityContains 1 MyTH4 domain. {ECO:0000255|PROSITE- ProRule:PRU00359}.
SimilarityContains 2 PH domains. {ECO:0000255|PROSITE- ProRule:PRU00145}.
SimilarityContains 3 IQ domains. {ECO:0000255|PROSITE- ProRule:PRU00116}.
Subcellular LocationCytoplasm, cytosol {ECO:0000269|PubMed:10984435}. Cell projection, lamellipodium {ECO:0000269|PubMed:10984435}. Cell projection, ruffle {ECO:0000269|PubMed:10984435}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:10984435}. Cell projection, filopodium tip {ECO:0000269|PubMed:10984435}. Cytoplasm, cell cortex {ECO:0000269|PubMed:10984435}. Cell projection, filopodium membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=May be in an inactive, monomeric conformation in the cytosol. Detected in cytoplasmic punctae and in cell projections. Colocalizes with actin fibers. Undergoes forward and rearward movements within filopodia. Interacts with microtubules.
SubunitMonomer, when in an inactive confomation in the cytosol. Homodimer in its active, membrane-bound conformation. Interacts strongly with CALM3 and weakly with CALM, the CALM3 interaction is essential for function in filopodial extension and motility. Interacts with ECM29. Interacts with NEO1. Interacts with ITGB1 and ITGB3. Interacts with VASP (By similarity). Interacts with DCC and ITGB5; the presence of DCC inhibits ITGB5 binding. Interacts with tubulin; ITGB5 or DCC binding inhibits tubulin binding. {ECO:0000250, ECO:0000269|PubMed:11278607, ECO:0000269|PubMed:20682791, ECO:0000269|PubMed:21321230, ECO:0000269|PubMed:21642953, ECO:0000269|PubMed:23012428}.
Tissue SpecificityUbiquitous. {ECO:0000269|PubMed:10984435}.
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