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MGP002585

UniProt Annotations

Entry Information
Gene Namearginyl-tRNA synthetase
Protein EntrySYRC_HUMAN
UniProt IDP54136
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative initiation; Named isoforms=2; Name=Complexed; IsoId=P54136-1; Sequence=Displayed; Name=Monomeric; IsoId=P54136-2; Sequence=VSP_018905;
Biophysicochemical PropertiesKinetic parameters: KM=3.9 uM for arginine (ATP-PPi exchange at 37 degrees Celsius) {ECO:0000269|PubMed:16055448}; KM=3.5 uM for arginine (arginylation at 37 degrees Celsius) {ECO:0000269|PubMed:16055448}; KM=1183 uM for ATP (ATP-PPi exchange at 37 Celsius) {ECO:0000269|PubMed:16055448}; KM=910 uM for ATP (arginylation at 37 Celsius) {ECO:0000269|PubMed:16055448}; KM=0.05 uM for calf liver tRNA-Arg (ATP-PPi exchange at 37 Celsius) {ECO:0000269|PubMed:16055448}; KM=0.41 uM for calf liver tRNA-Arg (arginylation at 37 Celsius) {ECO:0000269|PubMed:16055448};
Catalytic ActivityATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
DomainThe N-terminus (AA 1-72) has two regions predicted to be alpha-helical that might be involved in the multisynthetase complex assembly.
FunctionForms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1. {ECO:0000269|PubMed:17443684}.
InteractionQ9P2J5:LARS; NbExp=3; IntAct=EBI-355482, EBI-356077;
SimilarityBelongs to the class-I aminoacyl-tRNA synthetase family. {ECO:0000305}.
Subcellular LocationCytoplasm {ECO:0000269|PubMed:16055448, ECO:0000269|PubMed:16430231}.
SubunitInteracts (via N-terminus) with AIMP1 (via N-terminus); this stimulates its catalytic activity. Interacts (via N-terminus) with LARS2 (via C-terminus). Monomer; also part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with QARS. {ECO:0000269|PubMed:10358004, ECO:0000269|PubMed:16055448, ECO:0000269|PubMed:17443684, ECO:0000269|PubMed:24656866}.
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