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MGP003023

UniProt Annotations

Entry Information
Gene Namesulfotransferase family, cytosolic, 2B, member 1
Protein EntryST2B1_HUMAN
UniProt IDO00204
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; Synonyms=SULT2B1b, B; IsoId=O00204-1; Sequence=Displayed; Name=2; Synonyms=SULT2B1a, A; IsoId=O00204-2; Sequence=VSP_012510;
Biophysicochemical PropertiesKinetic parameters: KM=10.9 uM for DHEA (at 37 degrees Celsius, in the presence of 1mM MgCl2) {ECO:0000269|PubMed:16855051, ECO:0000269|PubMed:21855633}; KM=3.8 uM for DHEA (at 37 degrees Celsius, in the presence of 10mM MgCl2) {ECO:0000269|PubMed:16855051, ECO:0000269|PubMed:21855633}; KM=11.8 uM for pregnenolone (at 37 degrees Celsius, in the presence of 1mM MgCl2) {ECO:0000269|PubMed:16855051, ECO:0000269|PubMed:21855633}; KM=0.6 uM for PAPS (at 37 degrees Celsius, in the presence of 1mM MgCl2) {ECO:0000269|PubMed:16855051, ECO:0000269|PubMed:21855633}; Vmax=1752 pmol/min/mg enzyme toward DHEA (at 37 degrees Celsius, in the presence of 10 mM MgCl2) {ECO:0000269|PubMed:16855051, ECO:0000269|PubMed:21855633}; Temperature dependence: Optimum temperature is 37 degrees Celsius. Retains 70% and 20% of activity when incubated at 42 degrees Celsius for 45 and 120 minutes, respectively. Activity is lost after 200 minutes incubation at 42 degrees Celsius. {ECO:0000269|PubMed:16855051, ECO:0000269|PubMed:21855633};
Catalytic Activity3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-bisphosphate + an alkyl sulfate.
FunctionSulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol. {ECO:0000269|PubMed:12145317, ECO:0000269|PubMed:9799594}.
InteractionQ96Q35:ALS2CR12; NbExp=3; IntAct=EBI-749441, EBI-750451;
SimilarityBelongs to the sulfotransferase 1 family. {ECO:0000305}.
Subcellular LocationCytoplasm. Microsome. Nucleus. Note=Phosphorylation of Ser-348 is required for translocation to the nucleus.
Tissue SpecificityExpressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung. {ECO:0000269|PubMed:9799594}.
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